*2.4. RNA Extraction and cDNA Synthesis*

RNA was extracted from iSGECs, SGCLs, and Hela cells in 6-well plates using the RNAeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol. RNA was quantified using a Nanodrop 1000 (Thermo Fisher Scientific, Waltham, MA, USA). A total of 500 ng RNA from each sample was used per cDNA synthesis reaction. cDNA synthesis was carried out using the SmartScribe reverse transcription kit (Takara Bio, Mountain View, CA, USA) with random hexamers (20 μg) (Promega, Madison, WI, USA) and dNTPs (10 mM final) (Promega, Madison, WI, USA) according to the manufacturer's protocol. After synthesis, the cDNA was diluted to a final volume of 200 μL.
