**7. Conclusions**

The iSGECs generated in our study represent a preliminary model system for the development of therapies targeting salivary gland dysfunction. We have demonstrated that iSGEC-nSS2 cells derived from a sicca female patient retained the ability to form spheroids with differentiated cell types at late passages (p-80) and exhibited a significant proliferation capacity (>100 passages) when cultured as a monolayer. Overall, iSGECs could be used as an alternative to currently available cell culture models in salivary gland research.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2077-0383/9/12/3820/s1, Supplementary Table S1: RT-PCR primers; Supplementary Table S2: Antibodies and dilutions used in ICC and Western blots; Supplementary Figure S1: Semi-qRT-PCR analysis of SV40Lt expression in late-passaged iSGECs; Supplementary Figure S2A: Phase contrast images of iSGEC-nSS2 p-80 single-cell colonies; Supplementary Figure S2B–G: Phase contrast images of iSGEC-pSS1 and iSGEC-nSS1 spheroids; Supplementary Figure S3: expression of characterization markers in iSGEC-nSS2 cells by ICC.

**Author Contributions:** Experimentation, B.D.N., A.G.; conceptualization, B.D.N., J.-L.C.M., F.B.M., M.T.B.; manuscript preparation B.D.N., J.-L.C.M., F.B.M.; data analysis B.D.N., J.-L.C.M., F.B.M. All authors read and approved the final submitted version of this manuscript.

**Funding:** N/A. This work was supported by Atrium Health-Carolinas Healthcare Research Fund.

**Acknowledgments:** Salivary gland cell lines HSG and HSY were a generous gift from Bruce Baum (NIDCR, NIH). HMC-3A cells were a generous gift from Jacques E. Nör (University of Michigan School of Dentistry, Ann Arbor, MI, USA). We would like to thank our clinical research staff, Jenene Noll and Cathleen Peterson, for their help with patient recruitment, the collection of clinical data, and the collection and transport of LSG biopsy samples to the lab.

**Conflicts of Interest:** The authors declare no conflict of interest.
