*2.1. Culture of Salivary Gland Cell Lines (SGCLs) and HeLa Cells*

Salivary gland cell lines, HSG and HSY, and HeLa cells were cultured in DMEM supplemented with 10% FBS (VWR, Radnor, PA, USA) (37 ◦C; 5% CO2). A253 cells were cultured according to the provider's (ATCC, Manassas, VA, USA) recommendations [20]. HMC-3A cells were cultured according to the providers' recommendations [21]. Salivary gland cell lines (SGCLs) (HSG, HSY, A253, and HMC-3A) and HeLa cells were grown in T-75 tissue culture-treated flasks (Corning Life Sciences, Tewksbury, MA, USA) until 80–90% confluency. For experiments, SGCLs and HeLa cells were re-plated using 0.25% trypsin +0.053 mM EDTA (Corning Life Sciences, Tewksbury, MA, USA).
