**E**ff**ect of** *Candida intermedia* **LAMAP1790 Antimicrobial Peptides against Wine-Spoilage Yeasts** *Brettanomyces bruxellensis* **and** *Pichia guilliermondii*

#### **Rubén Peña, Jeni**ff**er Vílches, Camila G.-Poblete and María Angélica Ganga \***

Laboratorio de Biotecnología y Microbiología Aplicada, Departamento de Ciencia y Tecnología de los Alimentos, Universidad de Santiago de Chile, Santiago 917020, Chile; ruben.pena@usach.cl (R.P.); jeniffernatalia221@gmail.com(J.V.);camila.gonzalezpo@usach.cl(C.G.-P.)

 **\*** Correspondence:angelica.ganga@usach.cl;Tel.:+56-2-27184509

Received: 25 May 2020; Accepted: 19 June 2020; Published: 1 July 2020

**Abstract:** Wine spoilage yeasts are one of the main issues in the winemaking industry, and the control of the *Brettanomyces* and *Pichia* genus is an important goal to reduce economic loses from undesired aromatic profiles. Previous studies have demonstrated that *Candida intermedia* LAMAP1790 produces antimicrobial peptides of molecular mass under 10 kDa with fungicide activity against *Brettanomyces bruxellensis*, without affecting the yeas<sup>t</sup> *Saccharomyces cerevisiae*. So far, it has not been determined whether these peptides show biocontroller effect in this yeas<sup>t</sup> or other spoilage yeasts, such as *Pichia guilliermondii*. In this work, we determined that the exposure of *B. bruxellensis* to the low-mass peptides contained in the culture supernatant of *C. intermedia* LAMAP1790 produces a continuous rise of reactive oxygen species (ROS) in this yeast, without presenting a significant effect on membrane damage. These observations can give an approach to the antifungal mechanism. In addition, we described a fungicide activity of these peptides fraction against two strains of *P. guilliermondii* in a laboratory medium. However, carrying out assays on synthetic must, peptides must show an effect on the growth of *B. bruxellensis*. Moreover, these results can be considered as a start to develop new strategies for the biocontrol of spoilage yeast.

**Keywords:** antimicrobial peptides; *Brettanomyces bruxellensis*; *Candida intermedia*; *Pichia guilliermondii*; reactive oxygen species
