**5. Conclusions**

To sum up, most of the oenological di fferentiations due to inoculation strategies were observed in Tempranillo wines while in Graciano and Grenache changes due to di fferent inoculation strategies were scarce in many cases. Nevertheless, wine elaborated with di fferent wine varieties were perfectly identified considering the grape variety. This would be indicating that one of the most important previous step in obtaining not homogenous wines is the winemaking of di fferent grape varieties. Non-*Saccharomyces* early inoculated had been isolated from Tempranillo musts, so that a preadaptation to these grape variety properties might be expected. Therefore, changes in Tempranillo wines might be linked to the implantation or establishment rates of the inoculated yeasts. This research indicated, for the first time, that early inoculation with non-*Saccharomyces* should be carefully adjusted to the properties and features of a specific grape variety in order to increase the heterogeneity of the final product.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2311-5637/6/1/3/s1, One Figure S1 and one spreadsheet S1 has been included in this submission. The Figure S1 aims to clarify the sampling with a schematic representation and the spreadsheet S1 contains every data employed in statistical analysis.

**Author Contributions:** R.E.-V. was in charge of the methodology and the original draft preparation. P.G. was also responsible of the methodology. I.L.-A. was part of the research equipment. R.L. was a researcher of the project and also collaborated in the review and editing of the draft. P.S. researched and was in charge of resources. A.R.G. was responsible for the finding acquisition and for the project administration, and developed the formal analysis, resources and reviewed and edited the draft and eventually L.G.-A. submitted the manuscripts after reviewing and editing the draft. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Acknowledgments:** This work was supported by the Instituto Nacional de Investigaciones Agrarias.

**Conflicts of Interest:** The authors declare not conflict of interest.
