**5. Conclusions**

The present results indicated that sequential cultures of native non-*Saccharomyces* (*W. anomalus* 21A-5C, *M. guilliermondii* CLI 1217, and *M. pulcherrima* CLI 68 and CLI 460) with *S. cerevisiae* CLI 889 can be used as a strategy to reduce the ethanol levels in wines, whilst keeping the wine typicity of the area. These combinations could have a positive impact on glycerol content and the volatile profile of these wines, showing *W. anomalus* 21A-5C and *M. guilliermondii* CLI 1217 combinations with *S. cerevisiae* being mostly related to fruity and floral aroma compounds when compared with *M. pulcherrima* usage. However, further optimization will be required to control the acetic acid production in all sequential fermentations. Future work will focus on fermentations at a pilot scale through using the selected strains and having a second inoculation at different times, which will allow for the evaluation of the sensorial profile of the resulting wines.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2311-5637/6/2/60/s1, Table S1. Volatile acidity (as g/<sup>L</sup> of acetic acid), reducing sugars (glucose + fructose, g/L), and dry weight (mg) of wines after Section I (pure culture of non-*Saccharomyces* strains and the control under aerobic conditions).

**Author Contributions:** Investigation, M.G. and T.A.; methodology, M.G. and T.A.; formal analysis, M.G., B.E.-Z., J.M.C., and T.A.; writing—original draft preparation, M.G. and T.A.; writing—review and editing, M.G., B.E.-Z., J.M.C., and T.A. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was financially supported by the project RM2010-00009-C03-01 funded by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA).

**Conflicts of Interest:** The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; and in the decision to publish the results.
