3.2.1. Tempranillo

Results of the yeas<sup>t</sup> population found in Tempranillo are shown in Figure 2A. The initial indigenous yeas<sup>t</sup> population of Tempranillo must (day 0) was composed of 50% *Hanseniaspora* (*H*.) *uvarum*, 31% *S. cerevisiae* and the 19% remaining *T. delbrueckii* and *Cryptococcus* (*Cr*.) *laurenti*. The control sample of Tempranillo (C) before the second *S. cerevisiae* inoculation (day 3), was 30% *S. cerevisiae* and 70% *M. pulcherrima*. One day later (day 4), it was 77% *S. cerevisiae* and 23% *M. pulcherrima* and after a week (day 7) it was 100% *S. cerevisiae*. For samples early inoculated with *M. pulcherrima* (M), at day 3, the yeas<sup>t</sup> community was 60% *M. pulcherrima* and 30% *S. cerevisiae*, 7% *H. uvarum* and 3% *L. thermotolerans*. One day later (day 4), the yeas<sup>t</sup> community was composed of 70% *S. cerevisiae* and 30% *M. pulcherrima.* Eventually, after a week (day 7), all the identified yeasts were *S. cerevisiae*. In the case of Tempranillo grapes initially inoculated with *L. thermotolerans* and *T. delbrueckii* (*L*&*T)*, three days after their inoculation (day 3, the yeas<sup>t</sup> community was 87% *T. delbrueckii* and 13% *L. thermotolerans*. One day after *S. cerevisiae* was inoculated (day 4), it reached 40% of the yeas<sup>t</sup> community and 47% *T. delbrueckii* and 13% *L. thermotolerans* was found. Finally, a week after the first inoculation (day 7), all identified yeasts were *S. cerevisiae.*

**Figure 2.** Percentage of yeas<sup>t</sup> species in (**A**)Tempranillo, (**B**) Grenache and (**C**) Graciano, initial must (day 0), and control (C) samples and samples early inoculated with *M. pulcherrima* (M) and with a mix of *L. thermotolerans* and *T. delbrueckii* (*L*&*T*) during days 3, 4 and 7.

Considering the AF completed when the Brix degree had values between five and seven, the control AF of Tempranillo was completed in six days and the other two (M and *L*&*T*) lasted a day longer (Figure 3A).

**Figure 3.** Brix degree measurement during alcoholic fermentation of control (C) samples and samples early inoculated with *M. pulcherrima* (M) and with a mix of *L. thermotolerans* and *T. delbrueckii* (*L*&*T*); (**A**)Tempranillo, (**B**) Grenache and (**C**) Graciano.
