**4. Conclusions**

The antifungal supernatant obtained from de culture media of *C. intermedia* LAMAP1790 produces a continuous rise of oxygen reactive species (ROS) in *B. bruxellensis*, without trigger a significant effect on its membrane damage. This effect was totally avoided when the supernatant was treated with a proteolytic enzyme, proving that low mass peptides contained in this fraction are responsible for this effect. Herewith, *C. intermedia* L1790 showed antimicrobial effect on *B. bruxellensis* LAMAP2480, *Pichia guilliermondii* LAMAP3202, and LAMAP3203 when laboratory medium was used; however, similar effect was not observed when synthetic must was used. Therefore, it is necessary to identify the peptides with antifungal activity produced by *C. intermedia* LAMAP1790 and study how some enological factors (pH, ethanol, sugars, etc.) may affect their antifungal capacity. This will allow us to determine its possible technological application in the control of yeas<sup>t</sup> contaminants in the wine industry.

**Author Contributions:** Conceptualization: R.P. and M.A.G.; Methodology: R.P. and M.A.G.; Formal analysis: R.P. and J.V.; Data analysis: R.P., J.V., and C.G.-P.; Supervision: M.A.G.; Writing-Original Draft: R.P., C.G.-P. and M.A.G.; Project Administration: M.A.G. All authors have read and agreed to the published version of the manuscript.

**Funding:** Rubén Peña was funded by the Comisión Nacional de Investigación Científica y Tecnológica CONICYT-PCHA/DoctoradoNacional/2013-21130439 Doctoral Fellowship. Camila G-Poblete was funded by Facultad Tecnológica Fellowship from Universidad de Santiago de Chile.

**Conflicts of Interest:** The authors hereby declare they do not have any conflict of interest associated to this work.
