3.2.2. Grenache

The yeas<sup>t</sup> population identified in Grenache are shown in Figure 2B. The initial Grenache must was composed of 70% *H. uvarum*, 20% *S. cerevisiae*, and 10% of *M. pulcherrima*, *Pichia (P.) membranaefaciens*, *Aureobasidium (A.) pullulans* and *Williopsis (W.) anomalous (day 0)*. In control (C) samples analysed three, four and seven days after the first inoculation of *S. cerevisiae*, all yeas<sup>t</sup> isolates belonged to this species. Samples of Grenache inoculated with *M. pulcherrima* (M) were composed of 53% *M. pulcherrima*, 30% *S. cerevisiae* and 21% of *H. uvarum* and *Starmerella* (*St*.) *bacillaris* at day 3. One day later (day 4), 87% was *S. cerevisiae* and 13% *M. pulcherrima* and a week after the first inoculation (day 7), the entire yeas<sup>t</sup> community was identified as *S. cerevisiae*. Grenache inoculated with *L. thermotolerans* and *T. delbrueckii*

(*L*&*T*) were composed of 27% *H. uvarum*, 33% *S. cerevisiae*, 23% *T. delbrueckii* and 17% *L. thermotolerans* at day 3. The other two checks of implantation (days 4 and 7) showed that 100% of the yeas<sup>t</sup> community was *S. cerevisiae*. In the case of the Grenache variety (Figure 3B), the control sample either ended AF in six days and the others in seven days. The kinetics of the AF control sample were quicker than the AF of the samples early inoculated with the non-*Saccharomyces* yeasts.
