*2.4. Vaccine Preparation and Treatment Protocol*

One milligram of each peptide was emulsified in incomplete Freund's adjuvant (Montanide ISA-51VG; SEPPIC, Paris, France) and administered subcutaneously close to an axillary or inguinal lymph node, eight times weekly. Patients demonstrating no clinical or radiological progression without adverse events had the option of continuing to receive vaccinations at 2-week intervals, for up to 8 months after the initial vaccination.
