**5. Conclusions**

The high expressions of VEGF-A and VEGFR1 were observed in NF-PitNETs with CS invasion. Immunosuppressive microenvironments including TAMs and immune checkpoint molecules, which are induced by VEGF-A, were also associated with NF-PitNETs with CS invasion.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2077-0383/8/5/695/s1, Figure S1: Positive controls for each antibody.

**Author Contributions:** The following statements should be used "conceptualization, M.S., R.T. and M.T.; methodology, M.S., H.T., T.M., K.K., Y.M., and R.T.; software, M.S., and K.K.; validation, R.T., M.T., and K.Y.; formal analysis, M.S., K.K., and R.T.; investigation, M.S., H.T., T.M., M.T., K.K., Y.M., and R.T.; data curation, M.S., K.K., and R.T.; writing—original draft preparation, M.S., H.T., M.T., and R.T.; writing—review and editing, K.K., M.T., and K.Y.; visualization, M.S., and R.T.; supervision, K.Y.; funding acquisition, M.T.

**Funding:** This work was supported in part by grants from the Japan Society for the Promotion of Science (JSPS) (17H04306 and 18K19622 and to MT).

**Conflicts of Interest:** The authors declare no conflicts of interest.
