*4.8. Proliferation Assay*

Here, 90,000 NSCs were plated onto matrigel-coated 13 mm coverslips in P24 multiwell plates with NEM medium. At 48 h, 10 μM EdU was added to the media and incubated for one hour. After that, cells were washed with PBS, fixed with 4% PFA and permeabilized with 0.025% Triton in PBS. The Click-iT EdU Kit (Invitrogen, Carlsbad, CA, USA, C10338) was used for the detection of EdU, following the manufacturer´s instructions. Hoechst 33342 was added to stain nuclei. Quantification was performed manually at 40× in a randomized and blinded manner (*n* = t5).
