*4.7. Statistical Methods*

Statistical analysis was conducted on GraphPad Prism 8.0 using two-way ANOVA (Holm-Sidak) for biochemical assays and two-way ANOVA (Dunnett's) for tensile properties, with an α value of 0.05. All sample groups were tested against each other for biochemical assays, and tensile properties were compared against the rat bladder as a control.
