*3.5. Cell Viability*

Viable cells in proliferation and di fferentiation assays were quantified at the di fferent timepoints using a CellTiter 96 ® AQueous One Solution Cell Proliferation Assay (MTS, Promega, Madison, WI, USA). For that, the samples were incubated in an MTS:GM (1:5) solution for 3 h at 37 ◦C, under 95% humidified air and 5% CO2 conditions, and the absorbance at 490 nm was recorded with a microplate reader (Biotech Synergy HT, Winooski, VT, USA,). The quantitative results of proliferative cells will be presented as mean ± standard deviation (SD) and the results of the cells di fferentiation will be presented as mean ± standard error of mean (SEM), both of quadruplicated samples.
