*4.9. Immunofluorescence*

Cells were fixed with 4% PFA, permeabilized and blocked with 0.3% Triton X-100 and 3% Donkey Serum in TBS. Samples were incubated with the following primary antibodies overnight at 4 ◦C: MAP2 (Sigma, St. Louis, MO, USA, M1406,1:250); GFAP (Dako, Carpinteria, CA, USA, Z0334, 1:500); GAD65/67 (Abcam, Branford, CT, USA, ab11070, 1:500); KGA (Abcam, Branford, CT, USA, ab156876, 1:200); TUJ1 (Sigma, St. Louis, MO, USA, T8660, 1:400); MAP2 Chicken (Abcam, Branford, CT, USA, ab5392, 1:10000). Alexa Fluor Dye secondary antibodies were applied (1:500) at room temperature for 30 min. Images were acquired with a spectral confocal microscope LSM710 (Zeiss, Matesalka, Hungary).
