*4.5. Library Preparation and Sequencing*

The construction of the libraries and RNA-seq were performed by the Omicshare Biotechnology Corporation (Guangzhou, China). The mRNA was enriched with Oligo (dT) beads, then broken into short fragments and reverse transcribed into cDNA as templates. First and second-strand cDNA were then synthesized. The cDNA fragments were purified with a QiaQuick PCR extraction kit (Qiagen, Valencia, CA, USA) and end repaired. Poly (A) was added and ligated to Illumina sequencing adapters. They were then sequenced using the Illumina HiSeqTM 4000 (Illumina Inc., Centre Drive, San Diego, CA, USA).
