*2.4. Identification of Di*ff*erentially Expressed Genes and KEGG Enrichment Analysis of DEGs*

A total of 674 DEGs were detected in pairwise comparison with 424 downregulated and 250 upregulated genes (Non-spot-VS-Spot) (Figure 2). A total of 543 DEGs were mapped to all 106 pathways. Notably, the Flavonoid biosynthesis (ko00941, 12 DEGs of 105 gene with *Q-*value = 0.0005279107) and Phenylalanine metabolism (ko00360, 8 DEGs of 132 gene with *Q-*value <sup>=</sup> 1.900591 <sup>×</sup> <sup>10</sup>−1) were most significantly enriched in top20 pathways (Figure 3, Table S4).

**Figure 2.** Volcano plot of differentially expressed genes (DEGs). X axis: log2 transformed fold change; Y axis: −log10 transformed significance; Red points: upregulated DEGs; Blue points: downregulated DEGs. Gray points: non-DEGs.
