*4.6. Signal Pathway Phosphorylation Antibody Array Screening*

Cell lysates obtained from RAW264.7 cells treated with LPS (200 ng/mL) and with or without coelonin (2.5 μg/mL) were applied to a Phospho Explorer antibody Array PEX100, which was designed and manufactured by Full Moon Biosystems, Inc. (Sunnyvale, CA, USA). The microarray contains 1318 antibodies [52], each of which has two replicates that are printed on a coated glass microscope slide, along with multiple positive and negative controls. The antibody microarray experiment was performed according to the manufacturer's protocol. The protein phosphorylation level was measured as a ratio of the phospho and unphospho values.
