*4.4. Calculation of Ks and Ka Values of the* PLP\_deC Genes

The protein sequences of the gene pairs were first aligned using Clustal X 2.0, and then the multiple sequence alignments of proteins and the corresponding cDNA sequences were converted to codon alignments using PAL2NAL (http://www.bork.embl.de/pal2nal/) [40]. Finally, the resulting codon alignment was used to calculate Ks and Ka using DnaSP 5.0 (http://www.ub.edu/dnasp/) [41].
