*4.4. Sequence Analysis of PCR Products*

The amplified ITS regions of PCR samples were sequenced by Tsingke, Beijing, China. Sequence data were subjected to BLAST analysis on the NCBI (national center for biotechnology information) web tool to validate the characteristics of the amplified sequences along with isolates. All the sequences have been deposited in the GenBank under accession numbers i.e., *Colletotrichum tropicicola* ZF01: MN826680; *Fusarium keratoplasticum* ZF02: MN832906; *Fusarium oxysporum* ZF03: MN826681; *Fusarium solani* ZF04: MN826682; and *Trichoderma longibrachiatum* ZF05: MN826683. MEGA 7.0 software was used for phylogeny tree study.
