*2.6. Cell Culture*

The cell lines were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U mL−<sup>1</sup> penicillin, 100 μg mL−<sup>1</sup> streptomycin, and 2.5 μg mL−<sup>1</sup> amphotericin B. Cells were incubated in a humidified atmosphere of 5% CO2 at 37 °C. Sub confluent cells were trypsinized with trypsin-EDTA (0.25% trypsin, 1 mM EDTA) when monolayers reached 70% confluence.
