*2.7. Genotyping*

In order to determine the genotype of the Halothane (ryanodine receptor 1, *RYR1*) and Rendement Napole (protein kinase AMP-activated non-catalytic subunit gamma 3, *PRKAG3*), major genes that influence the reactivity of pigs to stress and pork quality, genomic DNA was isolated from blood collected in tubes containing EDTA as anticoagulant. Genotyping of the c.599G > A single nucleotide polymorphism (SNP) *PRKAG3* gene and the g.1843C > T SNP of the *RYR1* gene were done by PCR-RFLP analyses [26,27].
