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## *3.1. Instrumentation*

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#### **3. Materials and Methods**

#### *3.1. Instrumentation*

The home-made ZF setup (Figure 3) consisted of the following parts: a Minipuls3 peristaltic pump (Gilson), a micro-electrically actuated 10-port valve (Valco), a RF-551 flow-through spectrofluorimetric detector (Shimadzu); PTFE tubing was used for the connections of the flow configuration (0.5 or 0.7 mm i.d.), and Tygon tubing was used in the peristaltic pump. Control of the ZF system was performed through a LabVIEW (National Instruments) based program also developed in house.

The HPLC-PCD setup comprised the following parts (Figure S1 in supplementary material): a AS3000 autosampler (Thermo Scientific), a LC-9A binary pump (Shimadzu, Japan), a RF-551 spectrofluorimetric detector (λex/λem = 340/455 nm) (Shimadzu, Japan), an EliteTM vacuum degasser (Alltech, U.S.), a 150 × 4.6 mm i.d. reversed-phase column (Prevail, Alltech). A peristaltic pump was employed for the propulsion of the PCD reagents (Gilson Minipuls3). PTFE tubing (0.5 mm i.d.) was used for the construction of the reaction coil (RC) and all necessary PCD connections.

Data acquisition including peak highs (ZF method) and areas (HPLC-PCD method) was carried out through the Clarity ® *M*software (version 4.0.3, DataApex, Czech Republic). *olecules* **ŘŖŗş**ǰȱ*24*ǰȱ¡ȱȱȱȱ Şȱȱŗŗȱ

**ȱřǯȱ** ȱȱȱ ȱȱȱȱ ȱ ȱ ¢ȱȱ ȱ ȱ ȱȱ ȱ ȱ ȱDzȱȱ ƽȱȱǰȱ ȱ ƽȱ ȱǰȱȱƽȱǰȱȱƽȱȱǰȱȱƽȱȱǰȱȱƽȱȱǻ Ǽǰȱ ȱƽȱ ǰȱȱƽȱ*o*Ȭ¢ǰȱȱƽȱ *N*Ȭ¢¢ǯȱǯȱ **Figure 3.** Graphical depiction of the Zone Fluidics manifold and the analytical sequence steps for the determination of the adamantane derivatives; PP = peristaltic pump, HC = holding coil, S = sample, FL = fluorimetric detector, RC = reaction coil, C = carrier (water), W = waste, OPA = *o*-phthalaldehyde, NAC = *N*-acetylcysteine.
