*4.3. Lifespan and Heat Stress Assay*

Synchronized wild type nematodes were observed with a stereo microscope and scored for their survival by gently touching them with a platinum wire. The animals were counted daily from the first day of adulthood until all died. Contaminated plates as well as vanished nematodes were censored. The heat stress test was performed according to the lifespan protocol, however, nematodes were stressed by heat (37 ◦C) for 3 h at the third day of adulthood and counting of dead and alive worms started 1 day after stress exposure.
