*4.3. DNA Methylation Analysis*

For detection of DNA methylation, 500 ng DNA were subjected to bisulfite sequencing and hybridized to Illumina MethylationEPIC beadchip at the Technion Biomedical Core Facilities, Rappaport Faculty of Medicine, Haifa, Israel. Raw data underwent an analysis pipeline using Minfi [53] and BumpHunter [54] (Bioconductor R packages) for quality control and statistical analyses. PreprocessNoob was used for background correction through dye-bias normalization. DNAmTL and all age estimators were obtained using the online tool developed by Lu et al. (https://dnamage.genetics.ucla.edu/home) [37].
