*4.6. Statistical Analysis*

All experiments were independently conducted at least two times. The Online Application for Survival Analysis (OASIS 2; https://sbi.postech.ac.kr/oasis2/, accessed on 8 April 2020) [125] was used for comparing survival differences between two conditions. Fluorescence intensities as well as swim behaviour parameters were calculated as mean ± SEM, and statistical significance was calculated by a two-tailed t-test using GraphPad (https://www.graphpad.com/quickcalcs/, accessed on 8 April 2020). Chi-square test was used to compare the number of worms with intact and degenerated neurons in the UA44 strain.
