*4.5. Swim Behaviour Assay*

Wells with a depth of 0.5 mm and Ø 10 mm were created with two self-adhesive marking films for microscope slides and filled with M9 buffer. 5 to 10 nematodes were transferred per well, covered by a cover slip, and recorded for 60 s with a connected camera. Each video was converted into single frames which were analysed with the CeleST software as described by Restif et al. [47] and Ibáñez-Ventoso et al. [48]. The wave initiation rate, the body wave number and the activity index were evaluated as representative parameters of locomotive behaviour.
