**4. Conclusions**

In recent years, there has been a growing interest in collecting X-ray diffraction data directly from the crystallization plates to eliminate the crystal harvesting step. The use of the microplates and the grid scanning at SSRF enables efficient data collection and crystallization condition screening. Hundreds of crystals can be mounted on the goniometer head simultaneously by microplates, which bypasses the tedious step of mounting single crystals by a user or robot, and microcrystals can be efficiently aligned to the beam position for data collection. Additionally, the experiments can be conducted at room temperature and cryogenic temperature. Furthermore, this method is particularly attractive for fragile or optically invisible microcrystals. Our microplates are suitable for data collection for solving structures of multiple crystals at room temperature or cryogenic temperature.

**Author Contributions:** Conceptualization, Z.W.; Data curation, M.L. and H.W.; Funding acquisition, J.H.; Methodology, M.L. and L.Y.; Project administration, Q.W. and J.H.; Resources, B.S., H.Z., F.Y., Q.W. and J.H.; Software, Z.W.; Supervision, L.Y., B.S., H.Z., F.Y. and Q.W.; Validation, M.L.; Visualization, H.W.; Writing—original draft, M.L.; Writing—review and editing, Z.W. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by The National Key Research and Development Program of China (Grant No. 2017YFA0504901).

**Acknowledgments:** The authors thank the teams at the BL18U1 beamline of the Shanghai Synchrotron Radiation Facility for the beamtime allocated to this project and for their help in experiments.

**Conflicts of Interest:** The authors declare no conflict of interest.
