*2.2. Biocatalyst*

Amine transaminase from *Ruegeria pomeroyi*(as ECS-ATA08) was obtained as whole cell lyophilizate (Enzymicals AG (Greifswald, Mecklenburg-Vorpommern, Germany)) and was used as received during this study. The activity of 364 U/g was determined using the conversion of 2.5 mmol/L 1-phenylethylamine and 2.5 mmol/L pyruvate to acetophenone and alanine in 50 mM phosphate buffer pH 8.0 with 0.025 mmol PLP and 0.25 %(v/v) DMSO at 25 ◦C. One unit of enzyme activity is defined as the formation of 1 mmol acetophenone per minute, which was observed spectrophotometrically at 245 nm with ε(acetophenone) = 11.852 L/(mol·cm).
