*3.1. Crystal Growth in Microplates*

A typical result of crystal growth in microplate A with sitting-drop vapor-diffusion crystallization is shown in Figure 2a. Crystals can be clearly viewed from our beamline on-axis-video (OAV) system in Figure 2b. Conversely, crystal growth in microplate B with the hanging-drop vapor-diffusion method can also be achieved (Figure 2c,d). Using different desiccant in the reservoir well, we can grow different crystals in the microplates, which allows us to directly screen the crystallization conditions by the beamline video system. A typical result of the crystal growth under different crystallization conditions is shown in Figure 2e,f, which shows that crystallization conditions can be initially screened with microplate A and B under the microscope system (Figure 2e,f). Moreover, the suspension points or wells would be screened with grid scanning for further confirmation.

**Figure 2.** (**a**) Crystallization experiments, viewed under low magnification of the microscope, with plate A, the Kapton membrane is fixed on both sides of plate A to support crystallization experiments. (**b**) The growth of lysozyme microcrystals, viewed under high magnification of an on-axis-video system in a protein well of plate A, the length of microcrystal is less than 20 μm. (**c**) Crystallization experiments, viewed under low magnification of the microscope, with plate B, the Kapton membrane is fixed on the side where the centerline is of plate B to support crystallization experiments. (**d**) The growth of lysozyme microcrystals, viewed under high magnification of the on-axis-video system in a protein well of plate B. (**e**) The image of crystal growth under different crystallization conditions with plate A. (**f**) The image of crystal growth under different crystallization conditions with plate B.
