*2.3. Sample Loading*

The bottom of the microplates were coated with glue and tightly fixed into the magnetic base (Figure 1a,b). For the sitting-drop vapor-diffusion experiment with plate A, the reservoir solution in the reservoir wells frost at cryogenic temperature and affect the protein wells, therefore, in our experiment, plate A was directly mounted on the goniometer head for data collection at room temperature. During the rotation of plate A, it is difficult for the reservoir solution and the crystallization drops to flow out due to atmospheric pressure and liquid tension in the actual test. For the hanging-drop vapor-diffusion experiment with plate B, we removed plate B from the incubation chambers (Figure 1c,d), and then directly added 0.2 μL of glycerol to each large protein well and added 0.1 μL of glycerol to each small protein well. Finally, the side away from the centerline was sealed with a Kapton membrane, and then plate B was mounted on the goniometer head for data collection at cryogenic temperature.
