*4.1. Keratose-Coated Balloons*

The KOS-coated balloons were prepared as previously described [44,45]. Briefly, paclitaxel (LC Laboratories, Woburn, MA, USA) was prepared by dissolving paclitaxel in absolute ethanol followed by sonication at a final concentration of 40 mg/mL. Keratose (KeraNetics LLC, Winston-Salem, NC, USA) solution was prepared by dissolving lyophilized keratose in iohexol (GE Healthcare, Little Chalfont, UK) at a 6% weight-to-volume ratio in. An in-house air spray coating method was used to deposit keratose and paclitaxel in a layered approach on uncoated angioplasty balloons (Abbott Vascular, Abbott Park, IL, USA) [45]. Coated balloons were then sterilized by UV irradiation.
