4.3.4. H2O2 Content Determination

Shoot samples were extracted in a cold acetone for H2O2 content determination. One milliliter of extract was mixed with 1 mL of 0.1% titanium dioxide in 20% sulfuric acid and centrifuged at 8000 rpm for 15 min. The supernatant was then used to measure the absorbance at 415 nm. H2O2 content was calculated using a standard curve plotted in a range of 0.5–5 mM H2O2 and was expressed as mg g−<sup>1</sup> FW.
