*2.8. Determination of Non-Enzymatic Antioxidants in Di*ff*erent Plant Parts*

Ascorbic acid (ASA) content in different plant parts was determined following Mukherjee and Choudhuri [56] after extraction in TCA. The flavonoid contents in different plant parts were determined following the methods ascribed by Karadeniz et al. [57]. However, the total tocopherol content in different plant parts was assayed following the method of Backer et al. [58]. The contents of ASA, flavonoids, and tocopherol were measured quantitively using the standard curves prepared with known concentration of analytical grade ASA, rutin, and α-toc, respectively, obtained from Sigma-Aldrich Chemie GmbH - Schnelldorf, Germany.

#### *2.9. Determination Mineral Nutrients*
