2.7.2. Estimation of the Activities of Superoxide Dismutase, Peroxidase, and Catalase in Different Plant Parts

Activity of superoxide dismutase (SOD) was estimated using the method of Giannopolitis and Ries [54]. The method works based on the principle of photochemical reduction inhibition of nitroblue tetrazolium (NBT), which was used, and absorbance was read at 560 nm using an UV-visible spectrophotometer. However, the method of Chance and Maehly [55] was followed to measure the peroxidase (POD) and catalase (CAT) activities.
