*4.10. Data Analysis*

Fungal biomass estimation and quantitative analyses of acetamiprid were conducted in four repetitions, analyses of dtxs in six repetitions. Measurements of propidium iodide fluorescence were carried out in four repetitions. The mortality test of larvae of *T. molitor* was conducted in triplicates. Variabilities of samples were given as standard deviations (±SD). The one-way analysis of variance (ANOVA) and the posthoc Tukey test were used for investigations of statistical significance, using the concentrations of acetamiprid as a factor on transformed data. Scores at *p* < 0.05 were classified as significant. Statistica 13.1 (StatSoft, Tulsa, OK, USA) was used to analyze the data. The qualitative data of dtxs were submitted to PCA in orthogonal rotation and normalization using total area sums and Pareto scaling (Marker View Software 1.2.1., AB Sciex, Framingham, MA, USA).
