*4.7. Statistical Analysis*

Statistical analysis of data was carried out using IBM SPSS version 25 statistical software package (SPSS, Chicago, IL, USA). For comparison of categorical data, the Pearson chi-square and Fisher exact tests were evaluated in order to assess whether Enn B1 occurrence in several subgroups (age, gender, cereal consumption) were significantly di fferent. A non-parametric Kruskal–Wallis test was used to evaluate significant di fferences in Enn B1 metabolites concentrations. A confidence level of 95 % was assumed for examining data, whereas a *p*-value below 0.05 was considered as significant.

**Author Contributions:** Conceptualization, Y.R.-C. and A.R.; methodology, L.I. and A.N.; software, A.G. and G.G.; validation, L.I. and Y.R.-C.; formal analysis, L.I., A.N. and Y.R.-C.; data curation, A.G. and G.G.; writing—original draft preparation, A.N.; writing-review and editing, Y.R.-C.; supervision, Y.R.-C and A.R. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Acknowledgments:** Authors also acknowledge the technical support of Chiara Piemonte.

**Conflicts of Interest:** The authors declare no conflict of interest.
