**4. Conclusions**

In conclusion, the treatment with β-ZEL alone presented the highest cytotoxicological potency compared to treatments with the other mycotoxins assayed ( α-ZEL and BEA). The main type of interaction detected between mycotoxins for all combinations assayed was synergism. The potential interaction e ffects between combinations in this study are di fficult to explain since α-ZEL + BEA for binary and α-ZEL + β-ZEL + BEA for tertiary combination were found more in favor of synergic e ffect respect to CI value, compared with other combinations, which could be related to the concentration range studied, ratio in each mixture, exposure time assayed and cell line studied. Moreover, among all mycotoxins assayed, α-ZEL appeared to remain in the culture medium at the highest concentration and was less abile to ge<sup>t</sup> into SH-SY5Y cells compared to BEA and β-ZEL. In combinations, such e ffect observed for BEA reaching the highest in α-ZEL + BEA.

#### **5. Materials and Methods**
