*Article* **Biologically Active Metabolites from the Marine Sediment-Derived Fungus** *Aspergillus flocculosus*

**Anton N. Yurchenko 1,\*, Phan Thi Hoai Trinh 2, Elena V. Girich (Ivanets) 1, Olga F. Smetanina 1, Anton B. Rasin 1, Roman S. Popov 1, Sergey A. Dyshlovoy 1,3,4,5, Gunhild von Amsberg 4,5, Ekaterina S. Menchinskaya 1, Tran Thi Thanh Van 2 and Shamil Sh. Afiyatullov 1**


Received: 10 September 2019; Accepted: 9 October 2019; Published: 11 October 2019

**Abstract:** Four new compounds were isolated from the Vietnamese marine sediment-derived fungus *Aspergillus flocculosus*, one aspyrone-related polyketide aspilactonol G (**2**), one meroterpenoid 12-epi-aspertetranone D (**4**), two drimane derivatives (**7**,**9**), together with five known metabolites (**1**,**3**,**5**,**6**,**8**,**10**). The structures of compounds **1**–**10** were established by NMR and MS techniques. The absolute stereoconfigurations of compounds **1** and **2** were determined by a modified Mosher's method. The absolute configurations of compounds **4** and **7** were established by a combination of analysis of ROESY data and coupling constants as well as biogenetic considerations. Compounds **7** and **8** exhibited cytotoxic activity toward human prostate cancer 22Rv1, human breast cancer MCF-7, and murine neuroblastoma Neuro-2a cells.

**Keywords:** marine-derived fungi; secondary metabolites; polyketides; drimanes; meroterpenoids; cytotoxicity
