3.5.3. Elastase Release

Neutrophils (6 × 10<sup>5</sup> cells·mL−1) incubated in HBSS with MeO-Suc-Ala-Ala-Pro-Val-*p*- nitroanilide (100 μM) and Ca2+ (1 mM) at 37 ◦C were treated with DMSO or the tested compound for 5 min. Neutrophils were challenged by fMLF (100 nM)/CB (0.5 <sup>μ</sup>g·mL−1) for 10 min. The change in elastase release was spectrophotometrically measured at 405 nm (U-3010, Hitachi, Tokyo, Japan) [32].
