*4.5. Statistical Analysis*

Homoscedasticity of data was assessed by the Levene's test. After invalidation of this assumption for parametric analysis, we used generalized linear models (GLM) through a Poisson regression with a log link function, considering dilution, light/dark treatment, organ, and time (duration) of the experiment (in the case of the *Daphnia* assay) as explanatory variables. Analysis of variance based on deviance was carried out to assess the effect of independent variables on DNA damage and immobilization of *Daphnia*. Complementary comparisons were done with Student's *t*-test. All analyses were done using R [40]. Generalized linear models were computed with the package *glm2*.

**Author Contributions:** Conceptualization, M.D. and P.M.C.; laboratory methodology, M.D., A.C.S., A.A.-A.; data analysis, M.D., A.C.S., A.A.-A., A.J.P., P.M.C., writing—original draft preparation, M.D., P.M.C.; writing—review and editing, M.D., A.A.-A., A.J.P., P.M.C.; supervision, A.J.P., P.M.C., project administration, P.M.C. All authors have read and agreed to the published version of the manuscript.

**Funding:** The Portuguese Foundation for Science and Technology (FCT) funded projects GreenTech (PTDC/MAR BIO/01132014) and WormALL (PTDC/BTA-BTA/28650/2017). UCIBIO and LAQV are financed by national funds from FCT, references UID/Multi/04378/2020 and UID/QUI/50006/2020, respectively. The authors also acknowledge DPGM (the Portuguese General Directorate for Marine Policy) for funding the MARVEN project (ref. FA\_05\_2017\_007), which includes the fellowship attributed to M.D. A.A.-A. is grateful for the post-doctoral fellowship from Fundación Alfonso Martín Escudero.

**Acknowledgments:** The authors acknowledge A. Rodrigo, C. Martins, and C. Gonçalves (the SeaTox Lab at UCIBIO) for the important assistance during the work.

**Conflicts of Interest:** The authors declare no conflict of interest.
