*3.5. Generation of EXXR and CXG Sequence Logo*

CYP139 P450 family EXXR and CXG sequence logos were generated following the method described elsewhere [25,28,29]. Briefly, CYP139 P450 sequences were aligned using ClustalW multiple alignments using MEGA7 [76]. After sequence alignment the EXXR and CXG region amino acids (4 and 10 amino acids, respectively), were selected and entered in the WebLogo program (http://weblogo.berkeley.edu/logo.cgi). As a selection parameter, the image format was selected as PNG (bitmap) at 300 dpi resolution. The percentage predominance of amino acids at particular positions is calculated considering the total number of amino acids as 100%. The generated EXXR and CXG logos were used for analysis and compared to the different P450 family EXXR and CXG logos that have been published and are available to the public [25,28,29].
