*4.5. Transcriptome Analysis*

4.5.1. Library Construction and Sequencing, Illumina Read Processing, and Assembly and Annotation of Unigenes

According to the manufacturer's protocol for the TRIzol® Reagent (Invitrogen™, ThermoFisher Scientific, USA), approximately 100 nymphs and adults that had either been continuously selected with the LC25 dose of sulfoxaflor for six generations (SF-Sel) or not (Sus-Lab) were used for total RNA extraction. cDNA library construction and sequencing, Illumina read processing, assembly and bioinformatics analysis, and the annotation of unigenes, including protein functional annotation, pathway annotation, COG/KOG functional annotation and Gene Ontology (GO) annotation, etc., were performed as described by Wang et al. [46].
