*2.11. Quantitative PCR (qRT-PCR)*

The results indicated that the relative normalized expressions of *CYP6FD1* and *CYP4FD2* in the SF-Sel strain were 5.22- and 2.99-fold higher, respectively, than in the Sus-Lab strain, which were significantly higher than those of *CYP4FD1* and *CYP6FD2* (*p* < 0.05). The relative normalized expression of the *NlE78sf* transcription factor was highly significantly increased by 1.91-fold (*p* < 0.01), while the transcription factors *C2H2ZF1* and *C2H2ZF3* showed 1.53-and 1.30-fold increases, respectively, compared with the Sus-Lab strain (*p* < 0.05) (Figure 6).

**Figure 6.** The relative expression of DEGs in Sus-Lab/SF-Sel strains. Each RT-qPCR reaction for each sample was performed in three technical replicates. Asterisks indicate significant differences of up-regulated expression gene in SF-Sel strains compared to the Sus-lab strain (Student's *t*-test, \*\* *p* < 0.01 and \* *p* < 0.05).
