*4.9. Data Analysis*

The relative normalized expression of the upregulated P450 genes in the Sus-Lab and SF-Sel strains, the efficacy of *CYP6FD1* and *CYP4FD2* knockdown in 3rd-instar nymphs of the SF-Sel strain by RNAi, and the mortality of larvae injected with dsRNA with or without the LC50 concentration of sulfoxaflor were compared using analysis of variance (ANOVA) followed by Duncan's test for multiple comparisons (*p* < 0.05) with the SPSS version 17.0 software package (IBM).

**Supplementary Materials:** The following are available online at http://www.mdpi.com/1422-0067/20/18/4573/s1, (Transcriptome data) can be found at https://dataview.ncbi.nlm.nih.gov/object/PRJNA560503.

**Author Contributions:** Conceived and designed the experiments: X.-G.W. Performed the experiments; X.-G.W., Y.-W.R. and Xiang Xin. Analyzed the data: C.-W.G., Y.-M.Z. Contributed reagents/materials/analysis tools: L.-T.S. and Xiang Xu; drafted and revised the manuscript: X.-G.W., Y.-W.R. and C.-W.G. All authors approved the final version of the article, including the authorship list.

**Funding:** This research was financially supported by the National Key R&D Program of China (2018YFD0200300).

**Conflicts of Interest:** The authors declare no conflict of interest.
