**4. Conclusions**

Comparative analysis of P450s in bacterial species is gaining momentum and the availability of a large number of bacterial genome sequences is fueling this process. This study is an attempt to perform a comprehensive comparative analysis of P450s and to identify the P450s involved in secondary metabolite synthesis in *Bacillus* species. Future work involves understanding the role of different *Bacillus* P450s, identified in this study, in the synthesis of various secondary metabolites.

**Supplementary Materials:** Supplementary materials can be found at http://www.mdpi.com/1422-0067/19/11/ 3623/s1.

**Author Contributions:** K.S. conceived and designed the experiments; all the authors were involved in performing the experiments, analysis of the data and writing of the manuscript. All the authors reviewed and approved the manuscript.

**Funding:** Khajamohiddin Syed expresses sincere gratitude to the University of Zululand Research Committee for funding (Grant No. C686) and to the National Research Foundation (NRF), South Africa for a research grant (Grant No. 114159). Puleng Rosinah Syed thanks NRF, South Africa for DST-NRF Innovation Master's Scholarship (Grant No. 114575). Abidemi Paul Kappo is grateful to South African Medical Research Council (SAMRC) for research grant (Grant No. PC57009).

**Acknowledgments:** The authors want to thank Barbara Bradley, Pretoria, South Africa for English language editing.

**Conflicts of Interest:** The authors declare no conflict of interest. The founding sponsors had no role in the design of the study, the collection, analysis, or interpretation of data, the writing of the manuscript or the decision to publish the results.
