**4. Materials and Methods**

#### *4.1. Materials*

The human CYP2C8 gene cloned into the AmpR pAr5 (modified pCWOri+) plasmid was a gift from Dr. Eric Johnson. PCR reagents were purchased from New England Biolabs (Ipswich, MA, USA). Molecular biology enzymes and *E. coli* DH5α were purchased from Invitrogen (Waltham, MA, USA). Plasmid DNA was purified using a Qiagen Gel Extraction kit. Ampicillin (Amp), arabinose, chloramphenicol (Chlr), isopropyl β-D-1- thiogalactopyranoside (IPTG), and Ni-NTA resin were purchased from Gold Biotechnology (St. Louis, MO, USA). δ-Aminolevulinic acid (δ-ALA) was purchased from Frontier Scientific (Emeryville, CA, USA). 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Carbamazepine and paclitaxel were purchased from Cayman Chemicals (Ann Arbor, MI, USA). NADPH was purchased from P212121 Store.
