*3.3. Phylogenetic Tree Construction of Cyanobacterial Species P450s*

The phylogenetic tree of cyanobacterial species P450s was built using the methodology described elsewhere [54–56]. Firstly, the alignment of cyanobacterial species P450s protein sequences was performed by the MAFFT v6.864 program available at the Trex web server [75] (http://www.trex.uqam. ca/index.php?action=mafft). Then, the alignments were automatically subjected to tree inferring and optimisation by the Trex web server [76]. Briefly, the server inferred the trees with different algorithms, including maximum likelihood, maximum parsimony, neighbor joining, in the library, and searched out the best phylogenetic tree in the least-squares sense. Finally, the best-inferred tree was envisioned and coloured using iTOL [77] (https://itol.embl.de/).
