**5. Conclusions**

The effects of different enzyme combinations were demonstrated in the saccharification of sugarcane bagasse, soybean husks and oil palm empty fruit bunches submitted to mild acid and alkaline pretreatments. Sugarcane bagasse was efficiently saccharified after alkaline pretreatment, while soybean husks were efficiently saccharified regardless of the pretreatment method. Palm empty fruit bunches also showed similar responses of enzymatic saccharification independently of the pretreatment strategy; however, with much lower sugar yields. The best enzyme choice for sugarcane bagasse was B1 host, representing a cellulase/xylanase complex of *P. verruculosum*; for soybean husks, the combination of B1 host (80%) and B1-XylA (20%), this last one obtained through the recombinant expression of *P. canescens* xylanase A in the *P. verruculosum* B1 host strain, gave the best results; for palm empty fruit bunches, the B1 host preparation was the most efficient.

**Author Contributions:** Conceptualization, C.R.S. and A.P.S.; methodology, D.O.O., I.N.Z. and O.A.S.; validation, S.G.K.; formal analysis, D.O.O. and I.N.Z.; investigation, S.G.K. and M.V.S.; data curation, D.O.O., M.V.S. and O.A.S.; writing—original draft preparation, S.G.K.; writing—review and editing, A.M.R. and C.R.S.; supervision, A.P.S.; project administration, A.M.R. and C.R.S. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance code 88887.472495/2019-00, in the frame of the project Biotechnology Processes for the Production of Advanced Biofuels (BioADD), CAPES-PRINT UFPR, and in part by the Russian Foundation for Basic Research (RFBR No. 18-54-80027). The APC was funded by the Russian Foundation for Basic Research (RFBR No. 18-54-80027).

**Acknowledgments:** Authors are thankful to Julio César de Carvalho, Luciana Porto de Souza Vandenberghe, Adenise Lorenci Woiciechowski and Luiz Alberto Júnior Letti for administrative and technical support, and to the companies Biopalma Vale S.A., Usina Santa Terezinha and Imcopa for providing the substrates.

**Conflicts of Interest:** The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
