*2.1. Chemicals, Substrates and Enzymes*

*Escherichia coli* BL21 (DE3) was used for the expression of *fae5* and *fae6* genes harboured in pET28 plasmids. The cells were cultured in Luria-Bertani (LB) medium containing 50 µg/mL kanamycin at 37 ◦C. The induction was conducted by following the method described previously [20]. Enzymes were purified using 5 mL Ni-NTA Superflow Cartridges (QIAGEN®), purchased from QIAGEN (Germany), as per the manufacturer's instructions. Xylanase (Xyn11) from *Thermomyces lanuginosus* and ethyl ferulate (EFA) were purchased from Sigma Aldrich (South Africa). Insoluble wheat arabinoxylan (WAX) was purchased from Megazyme™ (Ireland).
