*2.4. Saccharification Conditions*

Three different combinations of enzyme preparations were tested, as presented in Table 3. Substrate concentration (in dry basis) was set at 100 g/L, in sodium acetate buffer (1 M, pH 4.5), and the reaction volume was 20 mL. The enzyme dosage for B1 host, B1-XylA and B1 host + B1-XylA was 10 mg protein/g dry substrate. In all cases, F10 β-glucosidase preparation was added to the reaction mixture in the dosage 40 U of pNPG /g of dry substrate. Hydrolysis flasks were incubated at 45 ◦C, 250 rpm and samples were taken after 3 h, 24 h and 48 h. Experiments were conducted in duplicates, with repetition.


### **Table 3.** Enzyme combinations.
