*2.5. Synergy Studies*

In order to study the synergistic interactions on agricultural residues, the FAE and Xyn11 combinations were first tested on WAX for the release of FA and XOS. Enzyme loadings for all single enzymes and all enzyme combinations were kept at a total protein loading of 4 mg protein per g of WAX and at 8 mg per g of CC. For combination experiments, an enzyme mixture consisting of Xyn11 and FAE in a 66:33% protein ratio was used. The enzymatic hydrolysis was performed at a substrate loading of 0.5% (*w*/*v*) for WAX and 1% (*w*/*v*) for CC in 50 mM phosphate buffer (pH 7.4) in a total volume of 400 µL. The reaction mixture was incubated at 40◦C with agitation at 25 rpm for 24 h and terminated by boiling at 100 ◦C for 5 min. Hydrolysis controls included reactions without the addition of enzyme or substrate. All the experiments were performed in triplicate. The amount of FA and *p-*CA released was determined using the HPLC method described in Section 2.4, while reducing sugars were measured using the DNS method and XOS were quantified using the HPLC-RID method described in Section 2.7.
