*2.2. Analytical Methods for Compositional Characterization of Samples, Inoculum and Mixture Introduced in the Reactor*

One of the sub-objectives of this research is to characterize the substrates in depth to know, draw conclusions and assist in the determination of the development of the process. For this purpose, the CrM substrate and the inoculum are characterized. In addition, the same compositional study is also performed to the mixture of substrate + inoculum introduced into the reactor, before the digestion test and after completion of the BMP test. In this way it can be compared and determined what changes in the composition has caused anaerobic digestion, such as how much organic matter has been degraded, whether the mixture has developed accumulation of (Volatile Fatty Acid) VFA, or how much volatile solids have been degraded.

In particular, the following compositional determinations according to the standardized methods were carried out (Table 1).
