**5. Conclusions**

The essential oil of *A. rosaeodora* showed activity against all the strains tested, with a lower minimum inhibitory concentration being observed for *S. aureus*. An efficient antioxidant activity of the essential oil was evidenced by the ABTS radical discoloration technique, fully inhibiting the radical in relatively low concentrations. These results point to an important potential for use as an antimicrobial and antioxidant agent. The antitrypanosomal activity of *A. rosaeodora* essential oil and linalool were observed at high concentrations against epimatigote forms, and even higher against intracellular amastigotes of *T. cruzi*. Both *A. rosaeodora* essential oil and linalool reduced nitrite levels in unstimulated cells revealing a potential effect in NO production. These data revealed the pharmacological potential of the *A. rosaeodora* essential oil and linalool, which encourage further studies.

**Author Contributions:** Conceptualization, A.M.T., J.V.S.-S., A.N.M. and F.A.-S.; methodology, A.M.T., J.V.S.-S., J.M.P.F. and S.C.M.; formal analysis, A.M.T., J.V.S.-S., V.E.M.F., S.C.M. and F.A.-S.; resources, K.d.S.C., A.L.A.-S., A.N.M., V.E.M.F. and F.A.-S.; data curation, A.M.T., J.V.S.-S., A.N.M., S.C.M., V.E.M.F. and F.A.-S.; writing—original draft preparation, A.M.T., J.V.S.-S. and F.A.-S.; writing—review and editing, A.M.T., J.V.S.-S., K.d.S.C., A.L.A.-S., A.N.M., V.E.M.F., S.C.M. and F.A.-S.; visualization, J.M.P.F., V.E.M.F. and F.A.-S.; supervision, A.N.M., V.E.M.F. and F.A.-S.; project administration, K.d.S.C., A.L.A.-S., A.N.M., V.E.M.F. and F.A.-S.; funding acquisition, K.d.S.C., A.L.A.-S., A.N.M., V.E.M.F. and F.A.-S. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the Coordination for the Improvement of Higher Education Personnel (Coordenação de Aper feiçoamento de Pessoal de Nível Superior do Brazil—CAPES) [grant number Finance Code 001]; and the Carlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro (Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro— FAPERJ) [grant number E-26/010.001759/2019]. The APC was funded by Oswaldo Cruz Institute (Instituto Oswaldo Cruz—IOC). Fernando Almeida-Souza is postdoctoral researcher fellow of CAPES [grant number 88887.363006/2019-00]. Dra. Ana Lucia Abreu-Silva is research productivity fellow of National Scientific and Technological Development Council (Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq) [grant number 309885/2017-5].

**Institutional Review Board Statement:** The experiments with animals were conducted in accordance with the guidelines for experimental procedures of the Conselho Nacional de Controle de Experimentação Animal (the National Council for the Control of Animal Experimentation) (CON-CEA) and approved by the Comissão de Ética no Uso de Animais (the Animal Research Ethics Committee) of the Instituto Oswaldo Cruz (CEUA-IOC/FIOCRUZ), License n<sup>º</sup> L018/2018.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** All datasets presented in this study are included in the article.

**Conflicts of Interest:** The authors declare no conflict of interest.
