**4. Conclusions**

In this study, 50 different strains of *Staphylococcus* spp. isolated from human semen were tested for susceptibility against 14 different essential oils alongside determination of their chemical composition. The antimicrobial resistance of the tested isolates was evaluated as well. The antimicrobial resistance of *Staphylococcus* spp. against chloramphenicol and tobramycin was found, while all isolates were sensitive to tetracycline and tigecycline. *C. luzonicum* (Blume) A. Gray exhibited a strong inhibitory effect; *A. balsamifera* L., *C. camphora* var. *linaloolifera* Y. Fuita, *L. cubeba* (Lour.) Pers., and *P. cabli* (Blanco) Benth. possessed a moderately inhibitory effect; and *B. carterii* Birdw., *C. camphora* (L.) J. Presl., *C. aurantium* L., *G. procumbens* L., *M. leucadendron* L., *M. ericifolia* Smith., *C. limon* (L.) Osbeck, *S. album* L., and *V. zizanoides* (L.) Roberty revealed no inhibitory activity on *Staphylococcus* spp. isolated from human ejaculates. As such, we may sugges<sup>t</sup> the use of the selected essential oils against *Staphylococcus* spp. contamination of human semen samples.

**Author Contributions:** Conceptualization, M.K. and E.T.; data curation, M.K., M.T., J.Ž., J.Š., P.Ł.K., and N.V.; investigation, M.K., M.T., J.Ž., T.S., and D.G.; methodology, M.K.; supervision, M.K.; writing—original draft, M.K., M.T., E.T., J.Š., J.Ž., T.S., D.G., P.Ł.K., and N.V. All authors have carefully revised and approved the final version of the manuscript.

**Funding:** This work was supported by grants APVV SK-BY-RD-19-0014 and BRFBR № X20SLKG-003 "The formulation of novel compositions and properties study of the polysaccharide-based edible films and coatings with antimicrobial and antioxidant plant additives".

**Acknowledgments:** The paper was supported by the Slovak Research and Development Agency project no. APVV-15-0544.

**Conflicts of Interest:** The authors declare no conflict of interest.
