**5. Conclusions**

*C. citratus* EO, citral, and geraniol were capable of inhibiting *E. coli* O157:H7 biofilm formation, decreasing cell adhesion and glucans production on stainless steel surfaces. This inhibitory effect could be related to an uncompetitive inhibition of glucosyltransferase activity caused by the presence of citral and geraniol. These results sugges<sup>t</sup> a possible inhibition mechanism of terpenes on biofilm formation of *E. coli* O157:H7.

**Author Contributions:** Conceptualization, I.V.-A., G.A.G.-A., M.A.M.-T. and J.F.A.-Z.; Data curation, L.A.O.-R.; Funding acquisition, J.F.A.-Z.; Investigation, L.A.O.-R.; Methodology, L.A.O.-R.and M.M.G.-P.; Resources, J.F.A.-Z.; Supervision, J.F.A.-Z.; Writing—original draft, L.A.O.-R. and M.M.G.-P.; Writing—review & editing, I.V.-A., G.A.G.-A., M.A.M.-T. and J.F.A.-Z. All authors have read and agreed to the published version of the manuscript. **Funding:** The authors are thankful for the financial support of the Mexican Council for Science and Technology CONACYT (Project number 60224). Ortega-Ramirez and Gutierrez-Pacheco acknowledge CONACYT for the received Doctoral Fellowships.

**Conflicts of Interest:** The authors declare no conflict of interest.
