**5. Conclusions**

In summary, the gene expression data obtained in this study reported the transcriptional changes in *P. rubens* genes influenced by the vapors of EOs. The study provides important findings regarding the understanding of the impact of EOs to fungal cells. The effects of EOs create a cascade of events that affect the metabolism and, consequently, the functions and structure of the fungal cell. It is evident that all the metabolic pathways, especially for vital functions such as polysaccharide and carbohydrate metabolisms, fatty acid metabolism, nucleotide and nucleoside metabolisms or the regulation of the production of SM, are deeply influenced by the antifungal properties of these natural compounds.

Each EO has different characteristics, and our study evidenced how some of them have more efficient antifungal activities and some others possess only a growth-inhibiting effect. Therefore, their application depends on the result that we wish to obtain, to kill or to mitigate. As natural extracts, they should be safe and can replace some more dangerous synthetic chemicals in order to disinfect indoor contaminated air. We have achieved precious experiences in the development and optimization of the microarray analysis, so it would be worth utilizing it in order to evaluate the antifungal properties of EO vapors against other indoor airborne fungi and compare the new data with this one.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/2079-6382/9/6/343/s1: Figure S1. Hierarchical clustering of differentially expressed genes, illustrated by the heatmap. Table S1. List of regulated genes. Table S2: List of genes and their corresponding metabolic pathways.

**Author Contributions:** Conceptualization, A.Š. and D.P.; methodology, Z.K., M.B., A.Š., A.P. and D.P.; software, A.Š. and G.B.; formal analysis, Z.K., A.Š. and D.P.; investigation, Z.K. and A.Š.; resources, D.P.; data curation, A.Š. and G.B.; writing—original draft preparation, Z.K., A.Š., G.B. and D.P.; writing—review and editing, Z.K., A.Š. and D.P.; visualization, A.Š. and G.B.; supervision, M.B., A.Š. and D.P.; project administration, D.P. and funding acquisition, M.B., A.P. and D.P. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by VEGA projects 2/0061/17 (Innovative disinfection strategies: the essential oils effect on microflora and materials of cultural heritage objects) and 2/0059/19 (Combination of nanoparticles and essential oils for mitigating the biodeterioration on various types of building materials).

**Conflicts of Interest:** The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses or interpretation of data; in the writing of the manuscript or in the decision to publish the results.
