*4.2. Titratable Acidity*

Cotyledons were harvested in the morning and evening, and frozen (−80 ◦C) until assayed. Leaf samples were weighed, ground in glass-distilled water, and titrated with 0.01 N KOH to a pH 7 endpoint as described by Guralnick et al. [20]. Results were expressed as μeq gFW<sup>−</sup>1.
