*4.4. Leaf Anatomy*

Cotyledons were harvested and cut into sections. They were fixed in 1.5% glutaraldehyde in sodium cacadylate buffer followed by fixation in osmium. Sections were dehydrated in a series to 80% ethanol. The leaf material for transverse cross-section was fixed with fluoroacetic acid and then embedded in JB4 plastic resin (2-hydroxyethyl methacrylate). Sections were cut with a microtome and stained with 1% aniline B.
