*2.4. Prediction of RNA Editing Sites*

The online PREP-Mt (predictive RNA editors for plants) suite of servers (http://prep.unl.edu/) was used to predict the possible RNA editing sites in the PCGs of *P. vulgaris* and the other four leguminous mitogenomes (*G. max*, *L. japonicus*, *V. radiata*, and *M. pinnata*). In order to predict more true RNA editing sites, the cut-off for prediction score was set as C = 0.2, which has been proven to be a slight optimum [46]. A low cut-off value will predict more true edit sites but will also increase the probability of misidentifying an unedited site as an edited one.
