*2.6. Phylogenetic Analyses*

To analyze the phylogenetic relationships of *Macrosolen* in Santalales, we constructed phylogenetic trees using 58 common protein-coding genes of 16 species and *matK* genes of 15 species by the MP and ML methods with a bootstrap of 1000 repetitions. The MP and ML trees were the same whether they were constructed by either common protein-coding genes or *matK* genes (Figure 8). All nodes in all the phylogenetic trees received a >50% bootstrap value. All four phylogenetic trees showed that the three *Macrosolen* species are sister taxa with respect to *S. jasminodora* (Olacaceae). *M. cochinchinensis*, *M. tricolor* and *M. bibracteolatus* were gathered into one branch with a well-supported bootstrap value (100%). The three species within the genus *Viscum* grouped with *Osyris alba* (Santalaceae) and all

Santalales species were clustered within a lineage distinct from the outgroup. As shown in Figure 8, the trees constructed by common protein-coding genes also received a higher bootstrap value than the trees constructed by the *matK* genes.

**Figure 8.** Phylogenetic trees constructed with the *matK* genes of 15 species by using the maximum parsimony (MP) (**A**) and maximum likelihood (ML) (**B**) methods. Phylogenetic trees constructed with 58 common protein-coding genes of 16 species using the MP (**C**) and ML (**D**) methods. Numbers at nodes are bootstrap values.
