*4.4. Other Analyses*

On the basis of the study of Zhou et al. [40], we analyzed the complete chloroplast genome of three *Macrosolen* species, including genome structure analyses (genome length, gene content and GC content), codon usage analyses, RNA editing site prediction, and repeat sequences analyses. The distribution of codon usage was investigated using the CodonW software (University of Texas, Houston, TX, USA) with the RSCU ratio [32]. Potential RNA editing sites were predicted using the Predictive RNA Editor for Plants (PREP-Cp, Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA) suite online program [44] with a cutoff value of 0.8. Simple sequence repeats were detected using the MISA software (Pgrc.ipk-gatersleben.de/misa/) [45]. Repeat sequences were identified by REPuter (University of Bielefeld, Bielefeld, Germany) [46].

**Supplementary Materials:** Supplementary materials can be found at http://www.mdpi.com/1422-0067/20/22/ 5812/s1.

**Author Contributions:** Conceptualization, H.Y. and Y.L.; methodology, L.N., L.W. and J.Z.; formal analyses, L.N., Y.C. and J.Z.; resources, Y.L. and Y.W.; data curation, L.N., Y.C., Z.X. and X.L.; writing—original draft preparation, L.N. and H.Y.; writing—review and editing, L.N. and H.Y.; funding acquisition, H.Y. and Y.L.

**Funding:** This research was funded by Major Scientific and Technological Special Project for "Significant New Drugs Creation" (No. 2018ZX09711001-008-007), Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences (CIFMS) (No. 2016-I2M-3-016) and Guangxi Natural Science Foundation (No. 2013GXNSFAA019120).

**Conflicts of Interest:** The authors declare no conflict of interest.
