*2.1. Complete Chloroplast Genomes of Three Macrosolen Species*

The length of the three studied chloroplast genomes ranged from 129,570 bp (*M. cochinchinensis*) to 126,621 bp (*M. tricolor*) with a typical quadripartite structure consisting of a pair of IRs (24,703–25,445 bp) separated by the LSC (70,692–73,052 bp) and the SSC (5320–5724 bp) regions (Figure 2). The three chloroplast genomes were found to highly conserved in GC content, gene content and gene order (Table 1 and Table S1). All three species comprised 113 genes, including 68 protein-coding genes, 35 tRNAs, eight rRNAs and two pseudogenes (*rps12* and *ycf2*). A total of 17 genes were found to be repeated genes, and 79 were found to be unique genes in the chloroplast genomes. Three genes (*clpP, ycf3 and rps12*) contained two introns, whereas 10 genes (*atpF*, *rpoC1*, *rpl2*, *rpl16*, *petB*, *petD*, *trnA-UGC*, *trnI-GAU*, *trnK-UUU* and *trnL-UAA*) had only one intron (Table 2 and Table S2).

**Table 1.** Length of chloroplast genome of three *Macrosolen* species and their base composition.


**Figure 2.** Gene map of the complete chloroplast genome of three *Macrosolen* species. Genes outside the large ring circle are transcribed in a counter-clockwise direction, and genes inside the circle are transcribed clockwise. The same color represents the same category of genes. Deep grey in the inner circle represents GC content, and lighter grey represents A/T content.


**Table 2.** Gene list of chloroplast genome of three *Macrosolen* species.

\* One or two asterisks following genes indicate one or two contained introns, respectively. (×2) indicates that the number of the repeat unit is two. The numbers in parenthesis at the line of 'Number' indicate the total number of repeated genes.
