*3.7. Targeted Protein Profiling of AQP1 and HSP70 in K133WT and K133AS-R Leishmania Parasites by Western Blotting*

β

Western blot analysis revealed that the expression of AQP1 was 1.6-fold higher in K133AS-R parasites whereas that of HSP70 was 5.46-fold lower in K133AS-R parasites as compared to K133WT parasites (Figure 7).

α μ α **Figure 7.** Expression analysis of AQP1 and HSP70 by Western blotting. Western blot analysis for the expression of AQP1 and HSP70 and α tubulin (endogenous control) protein was performed using 100 µgpromastigote cell lysates of K133WT and K133AS-R parasites. Proteins separated on a 12% SDS–PAGE gel, transferred to nitrocellulose membranes that were probed with anti-AQP1, anti-HSP70, or anti-α tubulin antibody followed by horseradish peroxidase (HRP)-conjugated antibody and developed using enhanced chemiluminescence (ECL).
