*2.4. Estimation of the Minimum Number of Replication Origins (MO)*

To estimate the MO needed to replicate an entire chromosome within the S-phase duration, we developed a mathematical inequation [8]. This formula uses as argument the S-phase duration (S) (which can be estimated by the CeCyD website), the size of the chromosome in question (N), and the replication rate (v). The lower bound MO for the number of origins required to replicate an entire chromosome is given by:

$$
\zeta\_{mo} \ge \lceil \frac{N}{2, v, S} \rceil. \tag{3}
$$

Of note, if the right-hand side of this inequation results in a fraction of a unit, then the next higher integer unit must be taken as the result of the inequation, which is represented by the ceiling function (⌈ ⌉).

For each organism analyzed, we used as parameters for the formula up-to-date data available in the TriTrypDB database (www.tritrypdb.org), NCBI database (www.ncbi.nlm.nih.gov), and data reported in the studies related [3,8,11–13,23–26] (see Table 1 for more details).
