*2.1. Leishmania Culture Conditions*

*Leishmania donovani* strain 1S [29] promastigotes and derived mutants were cultured at 25 ◦C in M199+ medium [30] with the respective antibiotics: puromycin (25 µg/mL, AppliChem, Darmstadt, Germany), blasticidin (5 µg/mL), G418 (50 µg/mL) and hygromycin B (50 µg/mL, all Carl Roth, Karlsruhe, Germany). Cells were passaged every 3–4 days.
