*2.2. Genomic DNA Isolation from Parasite Culture*

Genomic DNA (gDNA) from K133WT and K133AS-R promastigotes was isolated using a Wizard Genomic DNA purification kit (Promega, Madison, WI, USA) following the manufacturer's instructions. Quantification of the DNA was performed by optical density measurements in a Nanodrop and QubitFlex® 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). The quality of the gDNA was checked on 1% agarose gel for the single intact band.
