*2.2. Electrotransfection of Leishmania Parasites*

Electrotransfection and selection was performed as described [31]. Clonal parasite populations were obtained by limiting dilution in 96-well plates with an initial inoculum of 0.5 parasites/well in a final volume of 200 µL M199+ medium supplemented with the respective antibiotics and 1× penicillin/streptomycin (Sigma Aldrich, Munich, Germany).
