*2.7. Oligonucleotide Array*

Global mRNA expression profiling of K133WT and K133AS-R *L. donovani* was carried out usingsingle color microarray-based gene expression profiling. A high-density *Leishmania* multispecies 60-mer oligonucleotide array slide [8 × 15 K format] was used for the microarray experiment. The slide represented the entire genome of *L. infantum* and *L. major*. The microarray chip printed by Agilent Technologies (Santa Clara, CA, USA), contained a total of 9233 *Leishmania*-specific genes, including 540 control probes as described earlier [24,55,56].
