*2.3. Larvicidal Bioassay*

Larvicidal bioassays were adapted based on the methodology of the World Health Organization [4] with slight modifications. The second, third, and fourth instar larvae were transferred into 250 mL sterile plastic containers covering 25 mL of dosage treatments with different discriminate concentration of My-It (1 × 102, 1 × 104, 1 × 106, and 1 × 10<sup>8</sup> conidia/mL) with the blend of 24 mL de-chlorinated sterile water, along with 1 mL of mycotoxin dosage, and kept at 27 ◦C. This procedure was replicated three times and one control was kept for each replication, i.e., 20 larvae were used without any chemicals. The mortality of the larvae was documented 24 h post-treatments. The percentage of mortality was deliberate and mortality corrections, wherever required, were analyzed using Abbott's formula [23]. To determine population growth, water was treated with My-It 1 × 10<sup>3</sup> and newly emerged larvae were controlled. Each treatment was replicated five times. Percentage of mortality was recorded daily until death.

#### *2.4. Oviposition Deterrence Index*

Sub-lethal dosages of My-It (1 × 101, 1 × 102, 1 × 103, and 1 × 10<sup>4</sup> conidia/mL) were mixed thoroughly with 200 mL of rearing food in 300 mL glass jars to obtain the desired dosage for the experiments. The gravid females (20 nos.) were alienated equally between treated and control containers. Throughout the experiments, the female groups were kept isolated for 48 h in mosquito cages (25 × 25 × 30 cm). After counting eggs, the oviposition deterrence index (ODI) was calculated using the formula adapted from Hwang et al. [24].
