*2.9. Data Analysis*

Data from the mortality experiments were analyzed by analysis of variance (ANOVA) of arcsine, logarithmic, and square root transformed percentages, and data were expressed as the means of three replicates. Significant differences between treatment groups were analyzed by Tukey's multiple range test (significance at *p* < 0.05) using the Minitab®17 program. For enzyme activity, Microcal Software (Sigma plot 11) was used. The lethal concentrations required to kill 50% (LC50) of larvae in 24 h were calculated by Probit analysis with a dependability interval of 95% using the Minitab®17 program.

Mosquito longevity was analyzed using a log-rank χ2 test of equality over strata (PROC LIFE Table) along with Formula (1) (Allison [28], 1995) with Minitab® 17 statistical software package (Minitab, State College, PA, USA).

$$\text{Growth index} = \frac{\text{Percent survival of mosquito}}{\text{Duration of lane/adult larvae/adult NPV/adult}} \tag{1}$$
