*2.5. Fecundity Assay*

An identical number of unfed male and female (20 nos. each) dengue mosquitoes were used for fecundity experiments. They were introduced into cages for matting measuring (30 × 30 × 30 cm) at different sub-lethal dosages of My-It (1 × 101, 1 × 102, 1 × 10<sup>3</sup> and 1 × 10<sup>4</sup> conidia/mL). After receiving a blood meal, the eggs were collected daily using the small plastic containers containing water as an ovitrap in the cages. Three number of eggs laid in the ovitraps by the female was calculated.

The mean number of eggs laid in the ovitraps by the female (fecundity) was calculated by the number of the eggs laid in the ovitraps divided by the number of females. Adult death during the procedure was also measured.
