*2.1. Mosquito Culture*

The *Ae. aegypti* larval culture was maintained at the insect toxicology laboratory in St. Peters Institute of Higher Education and Research, Avadi, Chennai from 2019, without disclosure to any prior chemicals, and it was preserved at 26 ± 2 ◦C at 75–80% relative humidity (RH) under a photoperiod of 14L: 10D. Brewer's yeast, ChooStix Biskies-branded dog biscuits, and algae collected from pools

#### *J. Fungi* **2020**, *6*, 196

in a ratio of 3:2 were fed as a diet to the dengue larvae. The first-generation larvae were used for conducting experiments.

## *2.2. Isaria Tenuipes*

Isolation and maintenance of an *Isaria tenuipes* fungal strain were adapted from our previous research (Vasantha-Srinivasan et al. [15], originally obtained from MTCC (Institute of Microbial Technology (IMT), CSIR, Chandigarh, India). The culture was preserved in a potato dextrose agar (PDA) medium for 14 days at 27 ◦C. The obtained conidia from the suspension media were prepared using 0.1% Tween 80 diluted using double sterilized distilled water and the conidia were well spun for 20 min to avoid any clumpiness. The number of conidia was counted and we determined their active dosage using fluorescent microscopy (Optika Fluoroscence series B-600TiFL, Italy) at 10× using a Neubauer hemocytometer chamber. Several concentrations were prepared of 1 × 102, 1 × 104, 1 × 106, and 1 × 10<sup>8</sup> conidia/mL through dilution into double distilled water.
