*2.4. Incorporation of the Magnetoliposomes in Hydrogels*

All the used hydrogels were prepared for a final concentration of 0.4 wt% (4 mg/mL). Hereby, 1.2 mg of each compound was added to 150 μL of an aqueous solution 2 *v*/*v*% NaOH 1M and dissolved through agitation. After the compound dissolution, the hydrogel solution was taken out of the water bath and mixed with 150 μL of the prepared magnetoliposomes solution. To each mixture, 0.4 wt% of glucono-δ-lactone (GdL) was added under agitation, which led to a final pH of ~6–7. The mixture

was deployed in a fluorescence microcuvette and left cooling at room temperature, until the hydrogel was formed.

The curcumin release from hydrogels and magnetolipogels (300 μL) loaded with 0.05 mM curcumin was also assessed. The gels containing curcumin were prepared and left stabilizing overnight in Amicon® Ultra-0.5 mL centrifugal filters (MilliporeSigma, St. Louis, MO, USA) with 0.1 μm pore size. Then, pH = 7.0 buffer (800 μL) was added, and the filter tube was immersed and left standing at room temperature. Aliquots were taken after 7 h and fluorescence was measured to determine the concentration. The assays were performed in triplicate.
