*2.8. Drug Encapsulation*

A drop of water solution of amphiphilic dendron (10 μL, 10 mM) and a drop of water solution (10 μL, 10 mM) of a doxorubicin hydrochloride (DOX), sodium methotrexate (MTX) or 5-fluorouracil (5FU) were rapidly mixed in 1 mL of deionized water; an UV-vis spectrum of the sample **[As-mixed]** was recorded. The solution was sonicated for 30 min and then incubated for 2 days at room temperature; an UV-vis spectrum of the sample **[After treatment]** was recorded. Non-encapsulated chemodrug was removed by dialysis against deionized water (4 × 200 mL, 6 h); an UV-vis spectrum of the sample **[After dialysis]** was recorded. The degree of chemodrug encapsulation was estimated from UV-vis spectra as a ratio of a chemodrug absorption at λmax before and after dialysis. Drug loading capacity was estimated as a ratio of the weight of encapsulated drug to the total weight of nanoparticles neglecting the weight of the dendron washed off during the dialysis. The real loading capacity values are thus higher than estimated.

The UV-vis spectra of chemodrugs at different stages of encapsulation into dendrimersomes are shown in the Figure 1. DLS and zeta potential distribution profiles of drug-loaded dendrimersomes were recorded.

**Figure 1.** Structures of the chemodrugs used in the work: doxorubicin (DOX), methotrexate (MTX) and 5-fluorouracil (5FU) (**top**). UV-vis spectra of chemodrugs upon encapsulation into dendrimersomes (**bottom**).
