*2.2. Analytical and Spectroscopic Techniques*

1H and 13C and spectra were recorded on Varian Unity VXR-300 (Varian Inc., Palo Alto, CA) and Bruker AV400 (Bruker, Karlsruhe, Germany) instruments. Chemical shifts (δ, ppm) were measured relative to residual 1H and 13C resonances for CDCl3 used as solvent. ESI-TOF analysis was carried out in an Agilent 6210 TOF LC/MS mass spectrometer (Agilent, Santa Clara, CA, USA).

UV-vis spectra were recorded using an Eppendorf Biospectrophotometer (Eppendorf, Hamburg, Germany). Measurements were done at 25 ◦C using 1 mm thick quartz cells. Fluorescence spectra were recorded using a CLARIOstar microplate reader (BMG LABTECH, Ortenberg, Germany).

Hydrodynamic diameter of the supramolecular aggregates obtained was determined in plastic disposable microvolume cells using a Zetasizer Nano ZS particle analyzer (Malvern Instruments, Manchester, UK), equipped with NBS. The measurements were made at 25 ◦C. Zeta potential values were measured in plastic disposable cells DTS 1061 using Malvern Instruments Nanosizer ZS particle analyser. 10 mM of Na phosphate buffer was used to prepare solutions.

Transmission electron microscopy (TEM) images were obtained using a Veleta digital camera (EM SIS, Muenster, Germany) mounted on a JEM 1400 transmission electron microscope (JEOL, Tokyo, Japan) at the accelerating voltage of 80 kV. Samples were stained with 0.1% uranyl acetate.
