*2.6. CMC Measurements*

Association of dendron molecules into supramolecular constructions was studied by fluorescence spectroscopy using pyrene as a marker [32]. Aliquots of pyrene solution in acetone (5 μL, 20 μM) were dispensed in 200 μL glass vials and the solvent was removed under vacuum. Then, solutions of the amphiphilic dendron (100 μL, 0.3 to 100 μM) in 10 mM sodium phosphate buffer, pH 7.0 (PB) or 10 mM phosphate-buffered saline, pH 7.0–7.4 (PBS) were added. Samples were sonicated for 30 min and then incubated for 2 days at room temperature. After the incubation, the samples were transferred into the wells of 96-well black microplate COSTAR 96 half-area, and pyrene fluorescence spectra were recorded in the range 360–500 nm upon excitation at 345 ± 5 nm. The ratio of fluorescence intensities I373/I383 was plotted against <sup>−</sup>lgC, fitted with Boltzmann sigmoidal curve (r<sup>2</sup> <sup>&</sup>gt; 0.95), and the <sup>−</sup>lg(CMC) values were estimated from the fitting data.
