*2.12. Cellular Accumulation Assay*

For cellular accumulation assay, 4 h post-transfection cells were washed with PBS to eliminate fluorescently labeled unbound oligonucleotides. Next 24 h, cells were cultivated in full medium. Then, the cells were washed with PBS, trypsinized and suspended in PBS. The accumulation of oligomers was evaluated using NovoCyte (ACEA Biosciences, San Diego, CA, USA) flow cytometer. More than 5000 cells from each sample were analyzed using NovoExpress software (ACEA Biosciences).
