*2.11. Bioluminescence Imaging (BLI)*

BLI experiments were performed with an IVIS 100 imaging system (Perkin Elmer, Massachusetts, United States). The mice were injected intra-venously (i.v.) with D-luciferin (126 mg/kg body weight, Promega) dissolved in PBS (15 mg/kg). Afterwards, they were placed in the IVIS 100 imaging system and one image frame per second was acquired until a signal intensity plateau was reached. The following settings were used: 1 s exposure time, FOV of 10 cm, binning of 4, and an f/stop of 8. For in vivo quantification of fLuc reporter gene activity, the data were analyzed as photon flux per second (p/s) from a 1 cm<sup>2</sup> circular ROI located on the tumor using the Living Image software (version 2.50.1, Perkin Elmer). The signal intensity values at different time points (day 0, 1, 5, 8 and 15) were presented relative to the BLI signal of the same mouse at day 0.
