2.7.4. Holder and Stabilisation of the Tissue Samples

For measurements of the small tissue samples with the fresh tissue weights about 15–60 mg or even less in case of the aorta (about 10 mg), a method for their stabilisation in the straw holder was derived. Defrosted tissues were cut with cylindrical-shaped instrument with a diameter of 5.5 mm (3.5 mm for kidney) and was mounted on pre-weighted, 18 cm long copper wire with 0.2 mm diameter (Figure 8). The aorta was not cut, it was mounted by a wire embedded via the lumen of the artery. The sample was then vacuum dried for 1 h. During drying the sample shrunk and adhered to the wire. Diameter of the dried sample was then ~4.5 mm (~3 mm for kidney) and the sample attached to the wire was weighted, so we obtained the dry weight of the sample. The Cu wire itself is diamagnetic, long enough again to have negligible output signal to a magnetic moment of the sample.

**Figure 8.** Schematics of the sample configuration in holder with copper wire and a plastic straw.
