*2.1. Preparation of Manganese Ferrite Nanoparticles*

Manganese ferrite nanoparticles were synthesized by the co-precipitation method, as described in previous works [8,9,12]. Briefly, a mixture of 500 μL of MnSO4·H2O 0.5 M aqueous solution and 500 μL of FeCl3·6H2O 1 M was prepared and added, drop by drop, to a 4 mL NaOH 3.4 M aqueous solution at 90 ◦C, with constant magnetic stirring. After 2 h, nanoparticles purification was carried out by repeated centrifugations, dispersion in deionized water, and drying at 100 ◦C. The stability of nanoparticles dispersions in PBS medium (pH = 7.0) (with the same nanoparticle concentration used in the preparation of magnetoliposomes) was evaluated by following the UV/Visible absorption for one hour.
