*2.5. Cy3 Labeling*

The rAAV vectors were labeled using a Cy3 Ab Labeling Kit according to the manufacturer's recommendations by mixing rAAV (1 mL) in sodium carbonate/sodium bicarbonate buffer (pH 9.3) for 30 min at room temperature, followed by labeling with Cy3 and dialysis against 20 mM HEPES (pH 7.5)/150 mL NaCl [25].
