*2.5. Statistics*

Statistical analysis was performed using IBM SPSS Statistics 25 for all 4Pi microscopy image analysis. Statistical analysis for the acoustical characterization was performed using MATLAB. A Shapiro–Wilk test was used to assess the distribution of the data. For data that were normally distributed, a regular *t*-test was used to analyze the differences between groups. For all other data, the Mann−Whitney *U* test was used to test the difference between groups. Differences between groups were only tested for *N* > 2. Pearson's correlation tests were performed to assess the correlation between parameters.
