2.7.2. Trans-Corneal Permeation

Ex vivo permeation experiments were performed utilizing the vertical Franz diffusion cell using an isolated goat corneal membrane obtained from a local abattoir (Ahmedabad, India) held between the donor and receptor cell [31]. Testing were done using either optimized formulation (CL10) or control (0.25% drug solution equivalent to 2.5 mg of clarithromycin/mL). The receptor compartment was filled with simulated tear fluid (pH 7.4) with 1% Tween 80 (to maintain sink conditions), and temperature was set at 37 ± 0.5 ◦C. Suitable volumes of the samples were taken at specific time intervals and similar volumes of fluids at the same temperature. The samples withdrawn from the receptor compartment were appropriately diluted and estimated for clarithromycin by HPLC. The flux was determined as described in the literature [32].
