*2.7. Pig Skin Permeation*

Pig skin was obtained from a local abattoir (Barcelona, Spain) at the moment of sacrifice. Full thickness skin pieces were defatted (with a scalpel) and frozen at −20 ◦C until use. On the day of the experiment, skin pieces were mounted on Franz cells with an effective diffusion area of 0.196 cm<sup>2</sup> and around 12 mL of receptor volume capacity (PBS at 32 ◦C and stirred at 500 rpm). TEWL measurements were performed, to check the integrity of the skin samples [12] (TEWL Vapometer SWL4549, Delfin Technologies Ltd., Kuopio, Finland). A solution dose of 76 mg of each of the formulations were given in infinite dose in non-occluded conditions.
