2.4.4. Evaluation of Lipid Vesicle Flexibility

The flexibility of the different lipid vesicles was estimated indirectly by extrusion through a 100 nm membrane in cold. For this, 500 µL of each formulation (*n* = 3) was extruded 9 times at room temperature. The final collected volume was also recorded and the relative decrease ratio in particle size was calculated [54].
