2.5.1. Zeta Potential

The ZP was determined using a Nano-ZS Malvern Zetasizer (Malvern Instruments, Worchester, UK). A folded capillary was used for zeta-potential determinations with the instrument set in the Laser Doppler Anemometry (LDA) mode. The sample was prepared by dispersing 500-µL aliquots of the saturated nanoemulsion in 50 mL HPLC grade water and placed into a folded capillary cell. All measurements (*n* = 3) were performed at an applied field strength of 20 V/cm and the Helmholtz-Smoluchowski equation used to calculate the ZP of each sample, in situ.
