2.8.2. Preparation of Simulated Intestinal Lipolysis Media

FaSSIF was prepared by dissolving 2.240 g FaSSIF/FeSSIF/FaSSGF powder in 1L pH 6.5 ± 0.01 buffer (0.42 g NaOH, 3.4 g NaH2PO4, 6.2 g NaCl, 1L MilliQ). NaOH (1 M) and HCl (1 M) were used to adjust the pH of the intestinal buffer. Following preparation and before use, intestinal lipolysis media was left standing for 2 h to allow equilibrium of buffer excipients and micelles formation. On the day of the experimental procedure, pancreatin extracts were prepared by stirring 2 g of pancreatin powder in 10 mL of intestinal lipolysis buffer (pH 6.5) for 15 min, followed by centrifugation (2268 rcf, 20 min, 4 ◦C). The supernatant was collected in a glass vial and kept refrigerated.
