*3.1. Preparation and Characterization of Liposomes*

Modifications to our previously reported method [33] were used to prepare and characterize the PEGylated liposomes containing the potent microtubule-destabilizing agent 5,7-dibromo-*N*- (*p*-hydroxymethylbenzyl)isatin (*N*-AI; see Supplementary Information Figure S1 for chemical structure), surface functionalized ± PAI-2. The particle diameter, polydispersity index (PDI), peak intensity, and zeta potential for all liposome preparations are summarized in Table 1.

**Table 1.** Characterization of empty and *N*-AI PEGylated liposomes. Empty (EMP) liposomes, *N*-AI-loaded (*N*-AI) liposomes, empty PAI-2-functionalized (EMP PAI-2) liposomes, and *N*-AI-loaded PAI-2-functionalized (*N*-AI PAI-2) liposomes were prepared by the thin-film hydration method and analyzed by dynamic light scattering. Values are means ± s.d. (*n* = 3).

