*3.5. DHAH-NLCs Exhibited A Neuroprotective E*ff*ect*

After selecting the dose for generating a cell death of 50% after 24 h of incubation with 6-OHDA, 25 µM, we tried to demonstrate the neuroprotective effect of our DHAH-NLCs. The incubation of the dopaminergic neuron culture with DHAH-NLCs before and after 6-OHDA addition resulted in a neuroprotective effect in comparison to the neurotoxin itself (Figure 2C). The treatment with Mygliol-NLCs did not increase cell viability, with remaining living cell values similar to those obtained with just the neurotoxin incubation. The values for 50 µM Mygliol NLCs (38.35 ± 33.45), 25 µM Mygliol NLCs (49.49 ± 23.15) and 12.5 Mygliol NLCs (54.13 ± 19.11) were similar, without any neuroprotective effect. In contrast, for those cells treated with DHAH-NLCs, the values for the remaining living cells were similar to the control set as 100%. There were no statistical differences between the tested doses with the following values: 50 µM DHAH-NLCs (84.22 ± 10.58, \*\* *p* < 0.01), 25 µM DHAH-NLCs (82.70 ± 18.96, \*\* *p* < 0.01) and 12.5 µM DHAH-NLCs (79.92 ± 17.06, \* *p* < 0.05). The images taken with fluorescence microscopy showed the difference in living cells, comparing the ones treated with DHAH-NLCs with those treated Mygliol-NLCs (Figure 2D).
