**2. Material and Methods**

5-fluorouracil (5-FU, MW = 130.077 g/mol) was purchased from Sigma-Aldrich (Milan, Italy) and used as stock solution in ethanol (380 mM). The Liposomes Kit, composed of cholesterol (Chol, 9 µmol/package), L-α-Phosphatidylcholine (egg yolk, 63 µmol/package) and stearylamine (18 µmol/package), N-(3-dimethylamino-propyl)-N'-ethylcarbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide (sulfo-NHS) and phosphotungstic acid (99.995%) were purchased from Sigma-Aldrich (Milan, Italy). 1,2-stearoyl-*sn*-glycero-3-phosphoethanolamine-N- [carboxy(poly(ethylene glycol)-2000)] (DSPE-PEG2000-COOH) and 1,2-distearoyl-*sn*-glycero-3phosphoethanolamine-N-[methoxy(polyethylene glycerol)-2000] (DSPE-PEG2000) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Fetal bovine serum, penicillin/streptomycin and glutamine were purchased from Thermo-Fisher Scientific (Waltham, MA, USA). Polyclonal antibody against FZD10 was purchased from ABCAM (Cambridge, UK). Mouse monoclonal anti-human vimentin and rabbit polyclonal anti-human phospho-Paxillin were purchased from Cell Signaling Technology (Beverly, MA, USA) Goat anti-Mouse IgG (H + L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 and goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 were purchased from Thermo Fisher Scientific (Waltham, MA, USA) Prolong gold antifade reagent containing the nuclear staining 40 ,6-diamidino-2-phenylindole dichloride (DAPI) was purchased from Invitrogen (Carlsbad, CA, USA). CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Promega (Madison, WI, USA). All aqueous solutions were prepared using water obtained from a Milli-Q Gradient A-10 system (Millipore, 18.2 MΩcm, organic carbon content ≥ 4 µg/L). All other solvents and reagents were of analytical grade.
