2.4.2. Preparation of Super-SLH

This method was adapted from the previously established methods [29,30]. FEN was weighed into glass vials with 0.5 g of lipid to achieve concentrations equivalent to 80, 200, 400, and 600% Seq at room temperature. The glass vials were placed in the oven at 60 ◦C for approximately 10–30 min with intermittent shaking to dissolve the drug. Half a gram of silica microparticles (either FS or MPS) was added into the glass vial and immediately physically mixed to allow the mixture to form a white free flowing powder and allowed to cool. A ratio of 1:1 *w*/*w* silica: lipid was maintained throughout the study. Formulations were freshly prepared and were tested within 24 h.
