*2.2. HPLC Assay for Fenofibrate Quantification*

Fenofibrate was analyzed using a Shimadzu high-performance liquid chromatograph (HPLC) system (Kyoto, Japan) equipped with a Phenomenex Kinetex® 2.6 <sup>µ</sup>m PS C18 column (250 <sup>×</sup> 4.6 mm) maintained at 40 ◦C. The mobile phase contained 90% methanol and 10% water and was eluted at a flow rate of 0.7 mL/min. The effluents were analyzed by a UV detector at 288 nm, and the retention time for FEN was approximately 5.8 min. The concentration of FEN in each sample was determined using calibration curves generated over the range of 0.05–10 µg/mL with R<sup>2</sup> values > 0.999.
