*2.10. Light-Activated Release*

Photothermal calcein release was triggered using a compact single-mode laser module ML6700 (Modulight Inc., Tampere, Finland). Then, 500 µL of liposomes were incubated in HEPES buffer (37 ◦C), porcine vitreous (35 ◦C), or human plasma (37 ◦C) samples in Eppendorf ThermoMixer C (Eppendorf AG). After a sufficient incubation period, the sample was exposed to 800 nm laser for 5 or 15 s with the intensity of 3.2 W/cm<sup>2</sup> , based on our previous studies [18,21]. During the light exposure, the control tube was shielded from the laser. Right after the laser irradiation, the sample and the control were immediately cooled down in ice. The calcein fluorescence was measured with Varioskan LUX as described earlier and the release was calculated using Equation (3).
