*2.11. Liposome Stability in Vitreous and Plasma*

The storage stability of the PEG- and HA-coated liposomes was studied for one week in porcine vitreous and human plasma (25% liposome solution/75% biological fluid) samples. The mixtures were stored in the ThermoMixer C (Eppendorf AG) under 300 rpm shaking in tubes at 35 ◦C for the vitreous and 37 ◦C for the plasma samples, respectively. Samples were collected at time points of 0, 1, 3, 6, 48, 96, and 168 h, and their calcein leakage, ICG stability, and liposome size were determined as described above. Light-activated release was performed at the 3 h time point for vitreous (35 ◦C) and plasma (37 ◦C) samples, as well as at the 24 h time point for the vitreous samples.
