2.5.3. Transmission Electron Microscopy (TEM)

Transmission electron microscopy (TEM) was used to investigate the shape and surface morphology of the nanoemulsion and droplets in aqueous dispersions. Briefly, a drop of the aqueous nanoemulsion dispersion was placed onto a 3.05 mm copper grid fitted with a carbon film (FORMVAR/Carbon support 300 mesh) procured from TAAB Laboratories Equipment, Ltd., Alderson, Berks, RG7 8NA, UK. Excess liquid was removed using Whatman® 110 hydrophilic filter paper (Whatman® International, Ltd., Maidstone, UK) and the sample allowed to dry at room temperature (22 ◦C) for 24 h. The sample was visualized using a Zeiss® Libra Model 120 TEM (Zeiss, GmbH, Germany) operating at an accelerating voltage of 80 kV.
