2.5.4. Drug Load Determination

FEN content was determined by solvent extraction. Approximately 10 mg of formulation was weighed into a glass vial, followed by the addition of 10 mL of methanol. Vials were sonicated for 45 min to extract FEN. Aliquots were centrifuged (44,800 rcf for 20 min) to sediment undissolved solids. Then, the supernatants were appropriately diluted and analyzed using HPLC.
