*2.1. Materials*

Dipalmitoylphosphatidylcholine (DPPC), 1,2-distearoyl-*sn*-glycero-3-phosphocholine (DSPC), lysophosphatidylcholine (LysoPC), 1,2-distearoyl-*sn*-glycero-3-phosphorylethanolamine (DSPE), 1,2-distearoyl-*sn*-glycero-3-phosphoethanolamine-*N*-[amino(polyethylene glycol)-2000] (DSPE-PEG), 1,2-distearoyl-*sn*-glycero-3-phosphoglycerol (DSPG), and the 1,2-dioleoyl-*sn*-glycero-3-phosphoethanolamine -*N*-(Cyanine 5) lipid dye were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Citratephosphate-dextrose (CPD)-anticoagulated human plasma frozen within 24 h was bought from the Finnish Red Cross Blood Service (Helsinki, Finland), which is provided as an anonymized blood product for research use only that constitutes non-identifiable human material under the Declaration of Helsinki. Vitreous humour for proteomics experiments was isolated from porcine eyes supplied by HKScan

Finland Oyj (Forssa, Finland) and homogenized as described previously [29,31]. Triton X-100 was from MP Biomedicals (Santa Ana, CA, USA). Hyaluronic acid (HA; 8–15 kDa) and all other chemicals were bought from Sigma-Aldrich (St. Louis, MO, USA). Buffer and calcein solutions were made in advance of the liposome preparations. The buffer contained 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 140 mM sodium chloride (NaCl) in purified water, and the calcein solution for release studies had 60 mM calcein and 29 mM NaCl in purified water, both adjusted to pH 7.4 with sodium hydroxide (NaOH). Microwell dishes were from MatTek Corporation (Ashland, MA, USA).
