*2.10. Morphological Characterization by Transmission Electron Microscopy (TEM)*

For TEM investigation, liposomal formulations deposition was made onto 400 mesh amorphous carbon-coated Cu grids. For each sample, 5 µL of aqueous dispersion of LPs were dropped on the grid and the solvent was left to evaporate. Then, the grid was placed in contact with the top surface of a drop composed of an aqueous phosphotungstic acid solution (2%, *v*/*v*) for 30 s. Finally, ultrapure free water was used to wash the grid. TEM measurements were carried out using a Jeol JEM-1011 microscope, working at an accelerating voltage of 100 kV and fitted with an Olympus Quemesa Camera (11 Mpx).
