*2.3. In vitro Drug Release Study*

1 mL of FZD10-anti/5-FU/LPs was introduced into dialysis tubing (cut-off 3.5 kDa, Spectrapore) and dialyzed against 50 mL of PBS (10 mM and pH=7.4, outer medium). The dialysis was conducted at 37 ◦C in a water bath shaker. At defined time intervals, over 48 h, 100 µL of outer medium were collected, removed and replaced with fresh PBS. Each collected aliquot was lyophilized and solubilized in methanol; the drug concentration was determined by measuring the UV-Vis absorbance at 265 nm. The calibration curve described in the previous paragraph was used for the quantitative evaluation of the released drug. Measurements were conducted three times per sample.
