Disk Diffusion Method

Sterile filter paper discs of 5 mm in diameter were soaked with 100 µL of the FS-NTF and ethanol (99.9%) as test and positive control samples, respectively. The soaked paper disks were placed on inoculated Sabouraud dextrose agar plates. The inoculated plates were incubated at 24 ± 2 ◦C for 48 h and then examined for their respective zones of inhibition.
