*4.4. Part 2: Optimization of Instrumental Parameters by BBD*

The effects of instrumental parameters (emulsification speed, sonication amplitude, and number of HPH cycles) on dependent responses were evaluated by means of a BBD. A value of *R* <sup>2</sup> = 1 was obtained when 2-way interactions (linear quadratic) model was used (Supplementary Data, Section 3.2.1, Table S10 and Section 3.2.2, Table S11). Additionally, all variables were statistically significant (*p* = 0.001). Thus, the predicted and observed values were equal. The results are presented in 3-D response surface plots (Figures 2 and 3).

4.4.1. Effects of Emulsification Speed and HPH Cycles on Particles Size (Z-Ave, D50, and D90), PDI, ZP, and EE
