*4.4. Enzymatic Liposome Dialysis*

Liposome-supported enzymatic peritoneal dialysis (LSEPD) is the use of enzymeloaded liposomes (E-liposomes) to increase the functionality of PD. This technique was investigated in a rodent ethanol intoxication model [25]. Liposomes loaded with alcohol oxidase and catalase increased ethanol metabolism when compared with the free enzymes in dialysate, as evidenced by any increase in ethanol's primary metabolite, acetaldehyde, in both plasma and dialysate. E-liposomes also displayed less systemic exposure and organ distribution than the free enzymes in dialysate. Although LSEPD did not show significantly lower systemic ethanol levels in these rodent models [26], this work demonstrates that the peritoneal cavity could be utilised as an epicentre for these chemical reactions. Future work may focus on using this technique to increase the systemic clearance of target molecules, such as toxic alcohols.
