*3.8. Statistical Analysis*

Statistical analysis was performed using Statistica™ StatSoft software, version 13.5.0.17 (TIBCO® Software Inc). Results were statistically analyzed by ANOVA and a 2-way interactions model, with a 95% confidence level being considered statistically significant when the value of *p* was less than 0.05. Pareto chart, contour, and 3-D response surface plots were used to select the best formulation variables and the ideal conditions of each instrumental parameter to achieve the desired CQA values.
