*2.5. Immunofluorescence*

Primary cells were seeded at a density of 150 <sup>×</sup> <sup>10</sup><sup>3</sup> cells/well in 24-well culture plates with glass coverslips precoated with poly-l-lysine. At the time of interest, cells were fixed with 3.7% paraformaldehyde (PFA) for 10 min and, then, washed three times in PBS. Then, they were blocked and permeabilized with 1% (*w*/*v*) of BSA solution and 0.1% (*v*/*v*) Triton X-100 in PBS for 1 h at room temperature. After rinsing, they were incubated in rabbit polyclonal antibody Iba-1 (1:1000), mouse monoclonal anti-GFAP (1:2000) or rabbit polyclonal antibody TH (1:1000), respectively, with 1% (*w*/*v*) BSA and 0.1% Triton X-100 in PBS with agitation on at 4 ◦C. The following day, cells were incubated with the secondary antibody: anti-rabbit Alexa Fluor IgG 488 (1:1000) or anti-goat Alexa Fluor IgG 555 (1:1000), respectively, in PBS with 1% BSA and 0.1% Triton X-100 for 2 h at room temperature. After three rinsing steps, the fixed cells were incubated in DAPI (1:10,000) in PBS for 10 min. Then, they were washed twice with PBS and mounted with FluoromountTM.
