*2.8. Ocular Compatibility*

The biocompatibility of the optimized formulation (CL10) was performed in six albino rabbits (2–3 kg). The experiments were performed in accordance with the guidelines stated by the Committee for the Purpose of Control and Supervision of Experiments on Animals (approval number IP/PCEU/FAC/21/029, dated; 9 June 2017). Eye irritation score was measured according to the guidelines based on the Draize technique [34]. Single topical instillation of 50 µL of product (CL10) was applied on the left eye of each rabbit, whereas the same volume of physiological saline (reference) was applied on the right eye. The sterile formulation was tested two times a day for 21 days. The cornea, iris, and conjunctiva of rabbits were frequently observed during the study period. Similarly, signs of sensitivity reactions, particularly redness, swelling, cloudiness, edema, hemorrhage, discharge, and blindness, were checked [35].
