2.5.6. In Vitro Release

A 300-µL aliquot of a saturated EFV nanoemulsion was placed into a size-00 hard gelatin capsule for in vitro EFV release studies. The dissolution time of the capsules (*n* = 6) in dissolution medium at 37 ± 1 ◦C was approximately 8 ± 1.03 min. In vitro dissolution studies were conducted using USP apparatus II with a Hanson Vision® G2 Elite 8 dissolution bath fitted with a Vision AutoPlus and DissoScan™ auto sampler (Teledyne Hanson Research, Chatsworth, LA, USA) operated at a rotation speed of 50 rpm in 900 mL 0.1-M hydrochloric acid pH 1.2 with 1% *w*/*v* SLS at a temperature maintained at 37 ± 1 ◦C. A 10-mL sample was withdrawn at 15, 30, 60, 120, 390 and 720 min and filtered under vacuum with a Model A-2S Eyela aspirator degasser (Rikakikai Co., Ltd., Tokyo, Japan) through a 0.45-µm HVLP Durapore® membrane filter (Millipore® Corporation, Bedford, MA, USA) and the EFV released (*n* = 3) determined using the HPLC method described in 2.1. Sink conditions were maintained by replacement of fresh dissolution medium after removal of each sample.
