*2.5. Quantification of Metastasis and Statistical Analysis*

The metastasis affection in the lungs was quantified using ImageJ software. Upon lung extraction, the lungs were photographed, and lesions were quantified automatically using the ImageJ software. Values were compared using a Student's two-tailed *t* test statistical analysis. The significance was established for (\*) *p* = 0.001. The total number of events and the confidence levels obtained in the experiment (*n*) are all indicated in the figure captions. Results are expressed as mean values with their corresponding standard errors.
