*2.10. Cryogenic Transmission Electron Microscopy*

The structures formed upon the dispersion of the SEDDS 1:100 (*w*/*w*) in FaSSIF (Biorelevant, London, UK) at 37 ◦C were observed by cryogenic transmission electron microscopy (cryo-TEM). Individual samples (3.5 µL) were loaded on freshly glow-discharged TEM grids (Quantifoil, Cu, 200 mesh, R2/1) inside the Thermo Scientific Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA) and plunged frozen into liquid ethane. The Vitrobot chamber was kept at 4 ◦C and 100% relative humidity during the entire process. Each sample was incubated for 30 s on the grid and blotted for 5 s prior to vitrification. The grids were subsequently mounted into the Autogrid cartridge and loaded into the Thermo Scientific Talos Arctica transmission electron microscope (Thermo Fisher Scientific, Waltham, MA, USA). The microscope was operated at 200 kV, and cryo-TEM data was collected on the Thermo Scientific Falcon 3EC direct electron detector operating in charge integration mode. The micrographs were acquired using SerialEM software at magnifications corresponding to the calibrated pixel sizes of 22.16 Å/px and 3.15 Å/px, respectively. Each micrograph collected at 3.15 Å/px comprised an overall dose of 10 e/Å<sup>2</sup> .
