*3.1. Absence of NHERF1 Results in Extensive Changes in Kidney BBM Protein Expression*

BBM were prepared from the kidney cortex of WT and NHERF1 KO mice, followed by comparative proteomic analysis. Pathway analysis of differentially expressed proteins revealed significant changes in proteins associated with mitochondrial function as well as protein components of signaling pathways, actin cytoskeleton, cell survival, and oxidative phosphorylation (Supplementary Materials Figure S1, Table S1). Based on these findings, we proceeded to determine if metabolic pathways of WT and NHERF1 KO mouse kidneys differed. Under normal conditions, gluconeogenesis is a signature metabolic pathway for kidney proximal tubules with little glycolysis [35]. Therefore, we examined enzymes of the pathways involved in carbohydrate metabolism.
