2.5.3. CAT Activity

The (haemo)lysate was diluted 500-fold (100-fold for PLT) with 50 mM phosphoric buffer. Absorbance measurements of the study sample ((haemo)lysate and 30 mM H2O<sup>2</sup> solution; Sigma-Aldrich, St. Louis, MO, USA) were performed within 30 s at a wavelength of 240 nm (at 30 ◦C). CAT activity was determined from a calibration curve, which was obtained as a result of assays of several solutions with a known catalase activity pattern (Oxis Research, Portland, OR, USA).
