*3.6. DNA Binding and Degradation*

Separation of genomic DNA of fungi treated with Cu-Chit/NC gel by agarose electrophoresis is broken and DNA band are faint for selected samples, while no serious harm has occurred for untreated DNA. On the other side, the fungi treated with 180 ppm of nanocomposites a slightly less intense band can be observed compared to the untreated sample (Figure 10). The genotoxic effects for fungal mycelium were investigated after treatment with Cu-Chit/NCs gel DNA degradations was separated especially at high concentrations of Cu-Chit /NCs gel.

**Figure 10.** Agarose gel electrophoretic pattern of the fungal genomic DNA treated with 180 ppm of Cu-Chit/NCs gel, (**A**) Lanes 1–3: DNA for untreated *A. flavus* isolates, Lane 1: *A. flavus* (high producer (HP) isolate), Lane 2: *A. flavus* (intermediate producer (IP) isolate), Lane 3: *A. flavus* (low producer (LP) isolate). (**B**) Lanes T1, T2, and T3, three *A. flavus* isolates DNA treated with Cu-Chit/NCs gel, showed total damage to fragmented DNA bands.
