*2.4. Statistical Analysis*

Experimental data were subjected to one-way analysis of variance (ANOVA) and the differences between means were measured using Tukey's method. The values are given as means ± SD (standard deviations). Levels of significance were considered at *p* ≤ 0.05 unless otherwise stated, and the (L.S.D) at 5% level of probability using Co-state software [52].
