*2.3. Green Synthesis of MgO-NPs*

#### 2.3.1. Preparation of Fungal Biomass Filtrate

Three disks (0.8 cm in diameter) of the old culture of fungal strain E3 were inoculated into 100 mL of malt extract broth (MAB) media and incubated for 5 days at 30 ± 2 ◦C under 150 rpm shaking condition. At the end of the incubation period, the inoculated MAB was centrifuged and fungal biomass was collected. About 10.0 g of fungal biomass was resuspended in 100 mL dis. H2O for 48 h. at 30 ± 2 ◦C and 150 rpm shaking condition. The previous mixture was centrifuged at 10,000 rpm for 5 minutes and the supernatant (fungal biomass filtrate) was collected and used for green synthesis of MgO-NPs.
