Effect of Se-NPs on Oxidative Enzymes of *Vicia faba* under Pot Conditions

*Vicia faba* showed variation in relative mobility and density polypeptide bands as pathogenicity indicators and or treatment with Se-NPs, where healthy control (treatment 1) gave three isozyme bands with a low density of isozymes but soaking infected (treatment 4) and soaking *+* foliar spray Se-NPs (treatment 6) gave the same number of bands, three isozymes with moderate density. While infected control (treatment 2), as well as Se-NPs as a foliar spray on infected (treatment 8), gave three isozymes bands with a high density of isozymes, as shown in Figure 7, these results demonstrated that infected control recorded high activity as a high density of bands. Our results are similar to those of [90], who reported that the minimum activities of peroxidase enzymes were observed in healthy control. In this regard, Hasanuzzaman and Fujita [91] found that spraying with selenium increased the activity of many enzymes. In addition, [92] reported that nano selenium acts as a promoter and/or stressor, enhancing the antioxidant defense systems of plants, which leads to the improvement of plant tolerance under sandy soil conditions.

**Figure 7.** Effect of Se-NPs on peroxidase isozymes of *Vicia faba* under pot conditions 1: Control (H.). 2: Control (Inf.). 3: Soaking Nano (H.). 4: Soaking Nano (Inf.). 5: Soaking + Spray Nano (H.). 6: Soaking + Spray Nano (Inf.). 7: Spray Nano (H.). 8: Spray Nano (Inf.).



**Figure 8.** Effect of Se-NPs on Polyphenol oxidase isozymes of *Vicia faba* under pot conditions Control (H.). 2: Control (Inf.). 3: Soaking Nano (H.). 4: Soaking Nano (Inf.). 5: Soaking + Spray Nano (H.). 6: Soaking + Spray Nano (Inf.). 7: Spray Nano (H.). 8: Spray Nano (Inf.).
