2.7.1. Effect of AgNPs on Mycelium Growth

The inhibitory effect of AgNPs at four concentrations (10, 20, 30 and 40 µg/mL) on mycelium growth of *M. oryzae* strain Gry was determined using an agar medium test as described by [9], with slight modification. In brief, a disk (10-mm in diameter) of 7-day-old mycelium was inoculated in the centre of the petri dishes (9 cm in diameter), containing the blend of Potato Dextrose Agar (PDA) medium (pH 7.0), at several concentrations of AgNPs. The PDA plates were used without AgNPs as a control. The diameter of the fungus colony was measured after 7 d of incubation at 27 ◦C and then the inhibition of mycelium growth was calculated.
