*2.12. Binding*/*Degradation of Genomic Fungal DNA*

To check DNA quality, 10 microliters of *A. flavus* DNA were treated with Cu-Chit/NCs hydrogel (180 ppm) for 2 h at 37 ◦C. The DNA amplicon treated NCs gel was separated on 1.5% (*w*/*v*) agarose gels prepared in 1× Tris-acetate-EDTA (TAE) and stained with ethidium bromide (EtBr, 10 mg/mL). Five microliters of extracted and treated DNA from every pattern, along with 1 µL DNA loading dye, become loaded into the wells. Agarose gel was run for 30 min at 90 V and visualized to check for DNA degradation inside the GelDoc (Uvitec, Cambridge, UK).
