2.7.3. Effect of AgNPs on Spore Germination and Length of Germ Tubes

The influence of four concentrations (10, 20, 30 and 40 µg/mL) of AgNPs on the germination of spores and length of germ tubes of *M. oryzae* strain Gry were identified as described by [22], with slight modification. Briefly, 500 µL of spore suspension (1 × 10<sup>5</sup> spores/mL) that were prepared according to the method of [23], were added to the same volume of AgNPs in 1 mL tubes with a final concentration of 10, 20, 30 and 40 µg/mL. Mixed spore suspensions with ddH2O were used as the control. The germination rate of spores and length of germ tubes of *M. oryzae* strain Gry was recorded after 24 h of incubation at 28 ◦C in the dark, using the light microscope. The experiment was repeated twice with three replicates.
