*2.9. In Vitro Screening of Secondary Metabolites Produced from Mono and Co-Culture of Trichoderma spp. for Its Antibacterial Activity by Agar Well Di*ff*usion Method against Xanthomonas oryzae pv. oryzae (Xoo)*

The *Xanthomonas oryzae* pv. *oryzae* (Xoo) bacterial cultures were inoculated to 100 mL conical flasks containing nutrient broth and were incubated at 28 ◦C overnight. The petri plates were swabbed with Xoo cultures whose concentration was adjusted to 10<sup>8</sup> CFU/mL. The agar petri plates were made with the required number of wells using a sterile cork borer, ensuring the proper distribution of wells in the periphery and one in the center. Tetracycline was used as a positive control and distilled water as a negative control. The secondary metabolite crude extracts were loaded in each well. The plates were incubated at 28 ◦C for 24 h and after incubation, the plates were observed, and the results were recorded [44].
