*3.2. Aflatoxins Production Ability*

The production ability of toxigenic isolates was screened on four solid media. Beige rings seen without light are observed in aflatoxigenic fungal cultures. It is also possible to visualize the blue fluorescence ring that surrounds the aflatoxigenic colony under ultraviolet light. The aflatoxigenic *A. flavus* ◦C. The detected beige ring diameter and the strength of its fluorescence emission were improved under UV over time with the maximum observation by the end of the week (Table 1). It is possible to use AFPA media that is suitable for fast screen aflatoxigenic fungi associated with feeds. Four forms of aflatoxin activity have been quantified and measured (B1, B2, G1, and G2). Production patterns of AFs by aflatoxigenic *A. flavus* isolates are presented in Table 1. Thirteen of these were producers of AFB<sup>1</sup> and AFB<sup>2</sup> aflatoxins and 8 were nonproducers of aflatoxins. Thirteen of the isolates produced aflatoxin B1 ranging from 4.50 to 19.44 ppb, while B2 was produced in the same isolates with a 0.02–5.29 ppb. *A. flavus* (Af1) produced the highest AFB1 concentration (19.44 ppb) while *A. flavus* (Af13) produced the intermediate quantity of AFB1 (9.10 ppb) and Af13 produced the lowest quantity of AFB1 (4.50 ppb). None of the tested isolates produced aflatoxins G1 and G2.


**Table 1.** Fast screen aflatoxins by various cultural media and concentration of aflatoxins assayed by VICAM test in 21 *Aspergillus flavus* isolates collected from peanut meal and cottonseeds.


**Table 1.** *Cont.*

AF: Aflatoxin, +: Positive fluorescence, −: Negative fluorescence, ND: Not detected, *Aspergillus flavus* and *parasiticus* Agar (AFPA), Coconut agar (CA), potato dextrose agar (PDA), \*Af4, highly producer isolate. Af13, Intermediate producer isolate. Af19, Low producer isolate.
