*2.1. Collection and Isolation of Bacteria from Infected Rice Leaf*

The infected leaf samples were collected from different places of Karnataka and were subjected for the isolation of Xoo. The infected parts of the leaves were cut into 0.5 cm<sup>2</sup> , the surface was sterilized with 1% sodium hypochlorite for one minute followed by 3–4 sterile distilled water washes and was then blot dried. The sterilized leaf segments were inoculated onto an agar medium of yeast extract dextrose calcium carbonate (YDC) incubated at 28 ± 2 ◦C for 72 h. The plates were observed for convex, mucoid and yellow color [33].
