*8.6. Protocol of Green Synthesis Method*

The type of method(s) used for synthesizing NPs is a crucial parameter controlling the physicochemical and biological properties of NPs. Molnár et al. studied three different methods—extracellular fraction, autolysate of the fungal cells, and intracellular fractions for synthesizing Au-NPs using 29 thermophilic filamentous fungi [65]. NPs were formed using all three methods; however, the extracellular methods were the most acceptable, yielding NPs with a smaller size and low polydispersity. The authors also recommended washing the fungal mycelia several times before extracellular biofabrication to avoid the influence of residual growth media components on the NP synthesis process.
