2.3.3. In Vivo Assessment Efficacy of Se-NPs on *Vicia faba*

The inoculum of the pathogenic fungus *R. solani* was prepared according to Büttner et al. [48] that comprises mixing contents of 5 pure *R. solani* culture Petri dishes with 1000 mL of distilled water using electrical blender for two minutes. This experiment was carried out in the garden of Plant and Microbiology department, Faculty of Science, Al-Azhar University, Cairo, Egypt. The source of faba bean cv. Giza 716 was obtained from the Legume Research Department, Field Crop Institute, Agricultural Research Center, Egypt. The sandy loam soil was autoclaved (1.5 atm, 121 ◦C for 30 min) and distributed equally in disinfected pottery pots (30 cm in diameter) with 12 sterilized seeds per pot. The *Vicia faba* seeds were washed with distilled water then sterilized using 2% sodium hypochlorite for 2 min before conducting the treatments shown in Table 1.

**Table 1.** Treatments used in this study.

