*3.5. Titanium Dioxide Nanoparticles (TiO2)*

TiO<sup>2</sup> NPs were synthesized by using edible *P. djamor* mushroom and evaluated for anticancer potential against A-549 (human lung carcinoma) cell lines, as well as for larvicidal and bactericidal activity. Initially, 10 g of fresh biomass of *P. djamor* mushroom was washed with deionized water for 10 min and then cut to small pieces. Later, the chopped pieces were added in 100 mL of doubledeionized water, boiled at 60 ◦C for 15 min, and then filtered. Then, 20 mL of filtrate was added to 80 mL of TiCl<sup>4</sup> (5 mM) solution, stirred for 2 h, and kept to room temperature for 20 min until the color changed to brown. The intensity of the color of the extract was determined at the wavelength of 345 nm. The synthesized TiO<sup>2</sup> NPs formed, spherical in shape, with sizes of 31 nm [44].
