2.7.4. Effect of AgNPs on Appressorium Formation

The influence of four concentrations (10, 20, 30 and 40 µg/mL) of AgNPs on the appressorium formation of *M. oryzae* strain Gry was assessed according to [6], with slight modification. In brief, 500 µL of spore suspension (1 × 10<sup>5</sup> spores/mL) was added to the same volume of AgNPs in 1 mL tubes with a final concentration of 10, 20, 30 and 40 µg/mL. Spore suspensions with ddH2O were added as a control. The inhibitory effect of AgNPs on numbers and sizes of appressoria were recorded after 72 h of incubation at 28 ◦C in the dark, using the light microscope.
