*2.5. Aflatoxin-Producing Ability Medium*

Selected isolates from *A. flavus* (21 isolates) associated with dairy cattle feed samples collected from different governorates in Egypt were screened for aflatoxins production by four solid media. Four culture media recipes including *A. flavus* and *parasiticus* Agar (AFPA) (20 g/L yeast extract; 10 g/L bacteriological peptone; 0.5 g/L ferric ammonium citrate; and 15 g/L agar) [26], coconut agar (CA), PDA+ 20% NaCl, and PDA were employed to confirm aflatoxins production. All tested media were equipped as described earlier by some mycologists [1,27]. Aflatoxins production was detected via UV fluorescent light, light brown circle closed to fungal colonies was appeared after one-week incubation at 28 ◦C.
