2.4.2. Optical Research Microscopy Studies of the Fungal Hyphae

For the optical microscopy (Leica DM5000 B, Leica Microsystems, Germany) studies, fresh slides were prepared to observe the effect of ZnNPs on the hyphal morphology and structure representing the morphological damage caused to the fungi by the ZnNPs. The slides were stained with lactophenol-cotton blue dye to visualize the variations in the hyphal morphology at 200 and 400× magnifications and imaged (Leica DFC 420C, Germany).
