*3.2. Gold Nanoparticles (AuNPs)*

AuNPs synthesis was performed by using edible *P. florida* mushroom by the photo-irradiation method, and evaluated for anticancer potential against A-549, HeLa, K-562, and MDA-MB cell lines. Initially, 5 g of fresh biomass of *P. florida* mushroom was washed with deionized water and then cut to small pieces. Later, the chopped pieces were added in 500 mL of double-deionized water, under stirring, for half an hour. These contents were then incubated overnight. That content was then filtered via filter paper. Later, the filtrate of mushroom was used to reduce Au<sup>+</sup> into Au◦ in the presence of bright sunlight to form spherical to triangular-shaped AuNPs in the range of 10–50 nm [48].
