**3. Conclusions**

The wide range of agricultural products, contaminant ranges and different distribution ways make mycotoxin an important issue in the world. Concerns with potential human health impact associated with consumption of dairy products drive research in this field. Due to the complexity of animal feed products, developing a methodology for extraction and clean-up processes covering recognized mycotoxins is necessary. For this purpose, three analytical sample preparation techniques, immunoaffinity, solid-phase extraction and QuEChERS, were compared, and the best was optimized. Corn is considered to be a complex matrix with severe matrix interferences, and matrix-matched calibration was used to reduce ion source contamination and decrease matrix effect because of co-eluting matrix components. This LC-MS/MS method was designed to create a robust and reliable approach for simultaneous analysis of 14 mycotoxins in various feeds. These selected compounds with different degrees of toxicity are representative of an important group of mycotoxins. Recovery values ranged from 70–100% for DON, DAS, FB1, FB2, FB3, HT-2, T-2, OTA, ZON, AFG1, AFG2, AFB1 and AFB2 and 55% for NIV. The results from 5 AAFCO proficiency tests have been reported on various matrices along with in-house validation.
