*4.3. Fluorescent Detection of OTA*

Before the experiments, the diluted solution with H1 (1 µM) and H2 (1 µM) were heated at 95 ◦C by PCR for 5 min, then cooled to room temperature slowly at a rate of 1 ◦C/min to form a hairpin structure. Subsequently, different concentrations of OTA, H1 (300 nM), KCl (20 mM) were mixed with working buffer and heated at 37 ◦C for 15 min. H2 (300 nM) was then put in with a pipette and the reaction was continued at 37 ◦C for 60 min. After self-assembly was completed, NMM (1.5 µM) was added and further incubated for 10 min at 25 ◦C. Finally, a part of the solution was removed to a 96-well plate, and the fluorescence intensity was detected and recorded by the instrument mentioned above.
