*2.7. Accuracy and Precision of the ICA*

0

10

*2.7. Accuracy and Precision of the ICA* After the steps of pretreatment and being diluted at 10:1, the matrix effect for the spiked samples effectively decreased. The free of ZEN and 3 ng/g of ZEN in the spiked samples were judged to be negative (−), and 20 ng/g of ZEN was weakly positive (±) and 50 ng/g of ZEN was positive (+) using the ICA detection using naked eye, which we achieved by visualization and semi‐quantitative detection. Then, the spiked sample extracts were quantitatively detected by the ICA coupling strip reader in order to achieve digitization with ZEN that ranged from 2.6 to 59.3 ng/g. The quantitative recoveries of the spiked samples were between 86.7 to 118.6%, with the standard deviation (SD) being from 3.1% to 6.2%. These results indicate that the accuracy and precision of the ICA were desirable for the semi‐quantitative and quantitative detection for ZEN contamination in the cereal and feed samples (Table 2). After the steps of pretreatment and being diluted at 10:1, the matrix effect for the spiked samples effectively decreased. The free of ZEN and 3 ng/g of ZEN in the spiked samples were judged to be negative (−), and 20 ng/g of ZEN was weakly positive (±) and 50 ng/g of ZEN was positive (+) using the ICA detection using naked eye, which we achieved by visualization and semi-quantitative detection. Then, the spiked sample extracts were quantitatively detected by the ICA coupling strip reader in order to achieve digitization with ZEN that ranged from 2.6 to 59.3 ng/g. The quantitative recoveries of the spiked samples were between 86.7 to 118.6%, with the standard deviation (SD) being from 3.1% to 6.2%. These results indicate that the accuracy and precision of the ICA were desirable for the semi-quantitative and quantitative detection for ZEN contamination in the cereal and feed samples (Table 2).

3 − 3.4 113.3 ± 4.2 20 ± 18.6 93.0 ± 3.9 50 + 59.3 118.6 ± 5.5

3 − 2.6 86.7 ± 4.6

Wheat <sup>0</sup> <sup>10</sup> <sup>−</sup> ND ND

− ND <sup>c</sup> ND


**Table 2.** The ZEN recoveries from using the ICA method in the spiked samples.

<sup>a</sup> The visual detection was based on the ZEN levels: <sup>&</sup>lt;5 ng/g (−), 5 ng/<sup>g</sup> <sup>≤</sup> ZEN concentration <sup>&</sup>lt; 30 ng/g (±); <sup>&</sup>gt;30 ng/<sup>g</sup> (+). <sup>b</sup> Each value is the mean of three replicates. <sup>c</sup> Not detected.
