4.2.6. Linearity Studies

Using pure crystalline AFB1 (Fermentek Ltd., Jerusalem, Israel), 0.5, 0.4, 0.2, 0.1, 0.05 and 0.02 ng/mL AFB1 standards in 80% acetonitrile (HPLC Grade):water (Milli Q) (80:20):PBS-T mixture (10:65) were prepared, analyzed by AFB1-ELISA and the optical density (ODs) of duplicate wells read, as described in Section 4.1.5.
