Estimation of Intermediate Variability Associated with Wet Milling Procedure

Intermediate precision of the wet milling (slurry) method was also investigated. Five analysts were first trained, each randomly assigned three 25 g native pseudo blank feed aliquots, and each prepared a water slurry daily from one aliquot and weighed out one 1.3 g analytical sample as described above on three consecutive days. Each analytical sample was diluted 100-fold in acetonitrile:water (80:20, *v*/*v*), mixture agitated (Section 4.1.4), extracts diluted 10-fold in PBST and analyzed for AFB1 by ELISA as described above (Group X). This was repeated with another set of fifteen 25 g test portions, but each slurry aliquot was

diluted 66.5-fold in extraction solvent, agitated, diluted 7.5-fold in PBST and analyzed for AFB1 (Group XI).
