*4.7. Evaluation of the ICA*

Under the optimized vital parameters, the sensitivity of the ICA for ZEN would be improved. The two models for judging the results of the ICA could obtain semi-quantitative and quantitative detection. The corresponding minimum ZEN concentration, which could significantly reduce the red color in the T line compared with the reference, was defined as the semi-quantitative LOD. In the digitized ICA, the minimum ZEN concentration of the linear range was determined as the quantitative LOD. In addition, the cross-reactivity test and storage test were carried out to evaluate the specificity and stability of the developed ICA.
