*4.1. Reagents and Instrumentation*

All chemical reagents, including PAH, ProtA, mycotoxin standards, BSA, 2-amino-2-(hydroxymethyl)propane-1,3-diol (tris(hydroxymethyl)aminomethane, Tris) buffer and biochemicals were purchased from Sigma-Aldrich Co. (Dorset, UK or Budapest, HU), unless indicated otherwise. ZON-specific antibodies and protein conjugates were obtained at the Agro-Environmental Research Institute, National Research and Innovation Centre, Budapest, Hungary, as described before [4]. Immunoassays were performed in a Spectra-Max iD3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) using high-capacity 96-well microplates (Nunc, Roskilde, Denmark).
