**2. Sample Preparation and Handling to Support Method Accuracy**

As seen also from studies on method development in this Special Issue [11,12], sample preparation is a step of key importance in the chemical analysis process; not only due to its required features of applicability and recovery, but also because novel standardized

methods, such as the QuEChERS dispersive solid-phase extraction protocol can facilitate standardization of the analytical procedure improving inter-laboratory standard errors. The work of Kibugu et al. [19] clearly illustrates the importance of appropriate sample selection and preparation methods to maintain analysis performance quality descriptors including accuracy, precision, linearity, robustness, and ruggedness, as well as limits of detection and quantification. Their detailed statistical analysis of the determination of AFB1 content in chicken feed, using hierarchical sampling (from primary to quaternary with gradually decreasing sample sizes), wet milling with solvent extraction, and AFB1 quantification by a commercial ELISA kit, indicates accurate, precise, stable, reliable, and cost-effective analysis with improved inherent variability, which allows the processing of a lowered recommended test portion sample size of 50 g, and is suitable for laboratories not equipped with automated sample-splitting equipment.
