Comparison of Conventional (Dry) and Novel Water Slurry (Wet) Milling Procedure

To a 25 g feed aliquot, 1 mL blank methanol (HPLC Grade), 1 mL of 0.5 or 2.5 µg AFB1/mL solution in methanol (HPLC Grade) was spiked to achieve 0, 20 or 100 ppb AFB1 level, respectively. Group I aliquots were blended in a kitchen blender (Moulinex®, Model: Type LM240, Écully, France) at high speed for 1 min, while Group II aliquots were prepared by adding 37.5 mL of water (2.5-fold dilution) and blending as described above (Section 4.1.4), and 2 g slurry immediately weighed out. To each of the 25 g dry and 2 g slurry samples, 133 mL acetonitrile:water (80:20, *v*/*v*) was added and the mixture agitated

as described above (Section 4.1.4). Extracts of dry- and wet-blended aliquots were diluted 94.3-fold (final dilution factor = 500) and 7.5-fold (final dilution factor = 1247), respectively, in PBS-T and analyzed for AFB1 by ELISA as described above.
