4.2.3. Aflatoxin Spike and Recovery Studies

The solution of AFB1 (Fermentek Ltd., Jerusalem, Israel) in methanol (HPLC Grade) was spiked to achieve 20 or 100 ppb AFB1 level, respectively, in 25 g native pseudo blank feed aliquots, while blank methanol was added to native pseudo blank feed aliquots designated as "0 ppb".
