4.4.2. Study of the Effect of Incubation Time on Mycotoxin Binding of *Lactobacillus* Strains

The effect of incubation time was studied with 5 efficient AFB1 binding strains (TV3, TV24, MA2, TS23, SK63) selected by the results obtained in preliminary experiments (results not shown). In one case, the *Lactobacillus* strains were grown in 15 mL of MRS broth in the presence of 0.2 µg/mL AFB1 mycotoxin for 48 h at 37 ◦C. In the other case, the strains were cultivated in the same manner, but without the presence of the mycotoxin for 48 h at 37 ◦C, then the mycotoxin was added to the culture broths. The tubes were vortexed and incubated for 10 min at room temperature. The samples were then centrifuged at 4000 rpm for 40 min. The supernatant was decanted and the mycotoxin was extracted from the biomass (see Section 4.3).
