conditions. *3.6. HPLC Analysis of Aflatoxins*

*3.6. HPLC Analysis of Aflatoxins*  Samples were analyzed for AFB1, AFB2, AFG1, and AFG2 using a model 1100 HPLC system Samples were analyzed for AFB1, AFB2, AFG1, and AFG2 using a model 1100 HPLC system consisting of a degasser, an autosampler, and a quaternary pump, and equipped with a diode array

consisting of a degasser, an autosampler, and a quaternary pump, and equipped with a diode array

4.6 × 150 mm, 3.5 μm column with a temperature set at 30 °C. Samples were monitored at a wavelength of 365 nm for UV detection and at 365 nm excitation and 450 nm emission for FLD detection. The samples were eluted with the mobile phase of water (H2O)/methanol (CH3OH)/acetonitrile (CH3CN) (50:40:10) at a flow rate of 0.8 mL/m. The mobile phase was degassed and filtered through a membrane filter (47 mm × 0.45 μm) prior to use. The injection volume was 100 μL. Peak areas of AFs were recorded and integrated using the ChemStation software (Agilent).

1260 Infinity (DAD) and fluorescence 1260 Infinity II (FLD) detectors connected in series (Agilent Technologies, Santa Clara, CA, USA). The separation was performed via a Zorbax Eclipse XDB-C18 4.6 × 150 mm, 3.5 µm column with a temperature set at 30 ◦C. Samples were monitored at a wavelength of 365 nm for UV detection and at 365 nm excitation and 450 nm emission for FLD detection. The samples were eluted with the mobile phase of water (H2O)/methanol (CH3OH)/acetonitrile (CH3CN) (50:40:10) at a flow rate of 0.8 mL/m. The mobile phase was degassed and filtered through a membrane filter (47 mm × 0.45 µm) prior to use. The injection volume was 100 µL. Peak areas of AFs were recorded and integrated using the ChemStation software (Agilent).
