*2.6. Application to Real Contaminated Feedstu*ff*s*

The validated method was applied to the analysis of 99 feedstuff samples (Figure 5). DON and DON-3Glc were detected in 85% and 86% of surveyed samples and the mean concentrations were 511 µg/kg and 94.0 µg/kg, respectively (concentration ranging for DON between 10.1 and 1709 µg/kg and for DON-3Glc 1.70 and 385 µg/kg). Moreover, the ratio of the DON-3Glc concentration to that of DON ranged from 3% to 59% with a mean of 19%. The ratios of DON-3Glc/DON obtained in our study coincide with previously reported investigation [19,33]. The incidences of other toxins (3Ac-DON, 15Ac-DON, and NIV) were 35%, 26% and 23%, respectively.

**Figure 5.** The mycotoxins concentrations in contaminated feedstuffs (*n* = 99).
