*4.5. Determination of ZON by ELISA Method*

To confirm the utility of the immunosensor, ZON content was also determined in a corresponding competitive ELISA system [29]. ELISA plates were coated with 5 µg/mL ZON-BSA conjugate, and inhibition of binding of the polyclonal antibody by ZON was measured using a commercial horseradish peroxidase labelled second (anti-rabbit IgG) antibody and a colorimetric immunoassay signal measured at 450 nm.
