*4.12. Flow Cytometry Analysis of Apoptosis*

Cell apoptosis was evaluated by plating RTCC1 and J82 cells (10<sup>5</sup> cells each) with sh*LacZ* or sh*MAP1B* for 24 h, followed by 15 min of incubation using an Annexin V-FITC kit (BD Biosciences, Franklin Lakes, NJ, USA) that contained propidium iodide. The percentages of cells at late apoptosis were calculated from three independent experiments.
