*4.11. EC Fluids Generation*

Frozen tumor and adjacent healthy renal tissues were crushed into fragments <2 mm in diameter and suspended in 1:2 weight/volume of 2× phosphate-buffered saline (PBS). The solution was rotated at 4 ◦C for 3 h. The samples were then vortexed, and the centrifugation-cleared supernatants were used for experiments.
