*4.4. Construction of Expression and Luciferase Report Plasmids*

Mammalian expression plasmids for Flag-KLF5, HA-KLF5, pSG5-AR, Flag-AR, and luciferase reporter plasmids for pGL3-KLF5, pGL3-PSA, and pGL3-MMTV were generated in our laboratory. For promoter-luciferase plasmids, pGL3-AR, pGL3-AR1, and pGL3-AR2, primers were respectively designed with the entire 2-Kb immediate AR promoter sequence (AR), the upper 1.5-Kb (AR1) and the lower 0.5-Kb (AR2). PCR and cloning of PCR products were used to generate luciferase reporter plasmids for AR, AR1, and AR2 in pGL3-Basic vector following standard procedures. Primer sequences for gene cloning are listed in Supplementary Table S1.
