*4.9. Mouse Xenograft Studies*

C4-2B (2×107) cells in 100 <sup>μ</sup>L PBS-Matrigel (1:1) (BD Biosciences, catalog number: 354248) were implanted via subcutaneous injection into the flanks of mice. Cells were left to grow for one week. Mice were randomly divided into control or enzalutamide (10 mg/kg) (MedChemExpress, New Jersey, USA, catalog number: HY-70002) [58] groups, which were given once a day via oral gavage for up to 21 days (n=6/group). Mice were euthanized and tumors were surgically dissected, immediately weighed and fixed in 10% formalin for standard histopathological evaluation.
