*4.11. Flow Cytometry Analysis of Cell-Cycle Kinetics*

Stable pools of *MAP1B* knockdown versus the corresponding *shLacZ* control of the RTCC1 and J82 cell lines were pelleted and fixed overnight in 75% cold ethanol at −20 ◦C. The cells were washed twice using cold phosphate-buffered saline with 10 mg/mL of DNase-free RNase. Next, the cells were labeled with 0.05 mg/mL of propidium iodide and analyzed using a NovoCyte flow cytometer (ACEA Biosciences, San Diego, CA, USA) to determine the different proportions of cells at each phase of the cell cycle. Our lower limit of the number of sorted cells after gating out fixation artifacts and cell debris was 10<sup>4</sup> cells for all experiments.
