*4.5. Real-Time PCR*

Transcriptional knockdown efficiency was assessed 48 h post-transfection using quantitative real-time PCR. The following primer sequences were used: DONSON forward primer: 5- -gtccagcattgtagggcaac-3 and reverse primer: 5- -ggctctgctggaaggtacaa-3- ; β-Actin forward primer: 5- -CCAACCGCGAGAAGATGA-3 and reverse primer: 5- -CCAGAGGCGTACAGGGATAG-3- .
