*4.3. Western Blot Analysis*

Protein extracts of 20 μg were separated on a 4–20% SDS-PAGE gel and transferred to nitrocellulose membranes as described [10]. Band signals were obtained and visualized with the LI-COR Biosciences system (Lincoln, NE, USA). Antibodies: CPT1A: 15184-1-AP, (Proteintech, Rosemont, IL, USA); GAPDH: CST 5174, (Cell Signaling Technology, Beverly, MA, USA); SOD2: CST 13141, Cell Signaling Technology.
