*2.3. Survival Analysis in UTUC and UBUC*

During follow-up, we found in our UTUC cohort that 61 (17.9%) patients died because of their cancer and 70 (20.6%) patients experienced disease progression. During univariate analysis, we observed that multifocal tumors, advanced pathological tumor stage, positive lymph node metastasis, high histological tumor grade, the presence of vascular invasion, perineural invasion, and high MAP1B expression (Figure 3A,B) were associated with worse disease-specific survival (DSS) and metastasis-free survival (MFS) (all *p* < 0.05). In multivariate analysis, multifocal tumors, advanced pathological tumor stage, positive lymph node metastasis, high histological tumor grade, perineural invasion, and MAP1B expression were independently predictive for both DSS and MFS (all *p* < 0.05) (Table 3).

In our follow-up of UBUC patients, we found that 52 (17.6%) patients died due to the cancer and 76 (25.8%) patients experienced disease progression. During univariate analysis, we determined that advanced pT status, positive lymph node metastasis, high histological tumor grade, the presence of vascular invasion, perineural invasion, an increased mitotic rate, and increment of MAP1B expression (Figure 3C,D) were associated with worse DSS and MFS (all *p* < 0.05). Using multivariate analysis, we confirmed that advanced pathological tumor stage, an increased mitotic rate, and MAP1B expression remained significant in predicting reduced DSS and MFS (all *p* < 0.05) (Table 4).

**Figure 3.** Kaplan–Meier survival analysis showing the prognostic significance of MAP1B expression for the DSS and MFS outcomes of UTUC (**A** and **B**) and UBUC (**C** and **D**).


\* Statistically significant.

Univariatelog-rankandmultivariateanalysesforDSSandMFSin


**Table**

#### *Cancers* **2020** , *12*, 630
