*4.8. Bromodeoxyuridine (BrdU) Assay to Assess DNA Synthesis*

DNA synthesis was measured using an enzyme-linked immunosorbent assay (ELISA)-based and colorimetric bromodeoxyuridine (BrdU) assay (Roche Holding AG, Basel, Switzerland). *MAP1B*-knockdown or *shLacA* control RTCC1 and J82 cell lines were plated into a 96-well plate at a density of 3000 cells per well. At 24, 48, and 72 h, we measured the amount of DNA synthesis. The labeling medium was removed after three hours of incubation with BrdU at 37 ◦C under 5% CO2, followed by fixation and a final incubation with an anti-BrdU-POD solution. An ELISA reader

(Promega Corp., Madison, WI, USA) was used to measure the absorbance at 450 nm, and the reference was set at an absorbance of 690 nm.
