*4.6. RNA Interference*

The lentiviral vectors pLKO.1-*shLacZ* (TRCN0000072223: 5- -TGTTCGCATTATCCGAACCAT-3- ) and pLKO.1-*shMAP1B* (#1, TRCN0000116621: 5- -GCCTGGAATAAACAGCATGTT-3- ; #2, TRCN0000290688: 5- - CCCTGACTTAGGAGTTGTATT-3- ) were obtained from the Taiwan National RNAi Core Facility (Taipei, Taiwan) and used to establish stable *MAP1B*-silenced clones of RTCC1 and J82 cell lines using shRNAs against *MAP1B* (*shMAP1B*).Viruses were produced by transfecting HEK293 cells with the above three vectors using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) [29]. For viral infection, 3 <sup>×</sup> 106 RTCC1 and J82 cells were incubated with 8 mL of lentivirus in the presence of polybrene, followed by puromycin selection of the stable clones of lentivirus-transduced cells.
