*2.4. Antiproliferative Assay*

A549 and Hela cells (Produced from ATCC, Rockville, MD, USA) were cultured in DMEM (Dulbecco's Modified Eagle Medium), supplemented with 10% FBS, 100 mg/mL of streptomycin and 100 U/mL of penicillin at 37 ◦C in a 5%-CO2 humidified atmosphere. Cell cytotoxicity assay was done by MTT method [35]; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Concisely, cells were seeded in 96-well tissue culture plates and incubated for 48 h at 37 ◦C, 5% CO2. The culture medium was removed and replaced with fresh medium containing the synthesized compounds in different concentrations to the wells and incubated for another 24 h. Thereafter, the MTT solution (1 mg/mL) was added and incubated for 3 h. To dissolve the reduced MTT crystals, one hundred microliters of ethanol was added to each well, then, the optical density of each well was measured at 492/630 nm with enzyme immunoassay instrument.

### *2.5. In Silico ADME Prediction Analysis*

In this experiment, in silico ADME analysis was done in QikProp module of Schrodinger Maestro [36] where the important physiological descriptors like predicted IC50 for blocking hERG K+ channel in vitro, predicted octanol or water partition coefficient [log P(o/w)], number of hydrogen bond acceptors (HBA), number of hydrogen bond donors (HBD), predicted aqueous solubility (logS), MDCK cell permeability (MDCK), blood–brain partition coefficient (logBB), percentage human oral absorption rate, etc. were analyzed.

### **3. Results and Discussion**
