*2.2. Chemicals*

Solvents as methanol (analytical grade and for HPLC), trifluoroacetic acid, water (analytical grade and for HPLC) and reagents such as *Folin-Ciocalteu* and Chlorogenic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Ottawa Sand and Spe-edTM PSE Matrix-Hydroscopic Samples Dispersing Agent (Diatomee Sand), used as SFE-CO2 accessories, were purchased from Labservice Analytica S.R.L. (Italy).

Standards, for HPLC analysis and quantification, including Rutin, Isoquercitrin, Nicotiflorin and Narcissin were purchased from Extrasynthese (Lyon, France).
