*3.6. Total Phenolic Content (TP)*

Total phenolic content was determined according to the Folin-Ciocalteu method as described by Vondar et al. and Raupp et al. [19,44]. 200 µL of the extract was mixed with 1 mL of 0.2N Folin-Ciocalteu reagent, and 800 µL of Na2CO<sup>3</sup> (7.5%). The mixture incubated for 2 h in the dark at the room temperature, before reading the absorbance at 760 nm by using (Jenway 6405UV/VIS) spectrophotometer. The total phenolics expressed in mg/g as gallic acid equivalent based on the dry weight using a standard curve of gallic acid.
