*4.7. POX and PPO Activity Determination*

For the enzyme activity, the frozen material from −80 ◦C of storage was used. We were following the protocol described in Zupan et al. [40] for the preparation of samples for the POX activity and Cebulj et al. [41] for the preparation of samples for the PPO activity. Measurements were made according to the Worthington manual (Worthington Biochemical Corporation, 1972). The enzyme activity was calculated in ∆A min−<sup>1</sup> .
