*2.4. Total Phenolic Content*

The natural juice had the highest phenolic content value (11.58 mg/g) compared to the phenolics obtained in the juice extracts (Figure 2), being nine times higher than the obtained amount by Kazimierczak et al. [23] (1.29 mg). It is also worth to be mentioning that (OEM) revealed a high phenolic extraction for stems and peels, while no significant differences were detected in the total phenolics obtained by IEM and OEM in pomace and leaves. As mentioned previously, OEM, particularly ethanol (II), exhibited high extraction efficiency with stems recording 14.58 mg/g, compared to 9.35 mg/g obtained by ethanol (I). Whereas in leaves, ethanol (I) extracted 8.54 mg/g, which is close amount as that extracted by methanol (II) (8.12 mg/g). The obtained results were less than the results reported by Biondo et al. [8]. Phenolic compounds extracted from pomace by methanol (II) were 6.66 mg/g which was significantly higher than that reported by Shyamala and Jamuna [21] (2.2 mg/g), while less than that obtained by Canadanovi´c-Brunet et al. [ ˇ 27] (376.4 mg/g). However, methanol II extracted 9.96 mg/g from peels, which was less than that value reported by Kujala et al. [28] (15.5 mg/g). This discrepancy in the extraction capacity of different methods in different parts and plant origins might have resulted to the environmental biotic and abiotic stresses, which influence the presence and distribution of the phenolic compounds in the plant [26] and also might be resulted to the essence and nature of the phenolics compounds and their concentration in the plant materials.

**Figure 2.** Total phenolics in beetroot extracts (mg gallic acid/g); (I): IEM; (II): OEM at −20 ◦C.
