*3.9. HPLC Profile of Phenolic Compounds*

Phenolic compounds profile identified in natural juice, ethanolic (II) extract of peels, stem, and leaves by using an Agilent 1260 series HPLC with a multi-wavelength detector monitored at 280 nm. The injected 10 µL of each extract separated on a C18 column (4.6 mm × 250 mm i.d., 5 µm)

at 35 ◦C, and a mobile phase consisting of water (A) and acetonitrile (B) at a flow rate 1 mL/min. Where the mobile phase was programmed consecutively in a linear gradient as follows: 0 min (80% A); 0–5 min (80% A); 5–8 min (40% A); 8–12 min (50% A); 12–14 min (80% A), and 14–16 min (80% A). The unknown compounds characterized by matching its retention time to the standard's retention time.
