*4.3. Agronomic Evaluations*

At full seed maturity, four randomized sample areas of 2 m<sup>2</sup> were collected within each experimental plot for each crop and in each environment to assess harvestable crop yield. The plants were manually cut and gathered and then threshed by a fixed machine, using sieves suitable for small seeds, and evaluated for their moisture and seed yield.

#### *4.4. Seed Processing and Analysis*

Seed moisture was determined by oven-drying the seeds at 40 ◦C until constant weight for dry weight determination and the moisture content was calculated as the difference between the seed weight before and after the treatment. Oil was extracted by Buchi E-816 ECE (Soxhlet-like extractor) for 210 min, with hexane and *trans*-methylated in 2N KOH methanol solution [49]. Fatty acid profile was evaluated by a gas chromatography equipped with a flame ionization detector (Carlo Erba HRGC 5300 MEGA SERIES) and a capillary column Restek RT × 2330 (30 m × 0.25 mm × 0.2 µm), following the internal normalization method (ISO 12966–4:2015). The crude protein content was expressed as the percentage of dry matter and calculated from nitrogen using the conventional factor of 6.25. The obtained meals were dried at room temperature, vacuum-sealed and then stored away from light and heat, until the subsequent analysis.
