*Article* **Apple (***Malus domestica* **Borkh.) Cultivar 'Majda', a Naturally Non-Browning Cultivar: An Assessment of Its Qualities**

**Anka Cebulj 1,\* , Andreja Vanzo <sup>1</sup> , Joze Hladnik <sup>1</sup> , Damijana Kastelec <sup>2</sup> and Urska Vrhovsek <sup>3</sup>**

	- Hacquetova ulica 17, 1000 Ljubljana, Slovenia; andreja.vanzo@kis.si (A.V.); joze.hladnik@kis.si (J.H.)

**Abstract:** Browning of apple and apple products has been a topic of numerous research and there is a great number of methods available for browning prevention. However, one of the most efficient ways, and the one most acceptable for the consumers, is the selection of a non-browning cultivar. Cultivar 'Majda' is a Slovenian cultivar, a cross between 'Jonatan' and 'Golden Noble'. In this study, it was thoroughly examined and compared to the well-known cultivar 'Golden Delicious' with the aim to decipher the reason for non-browning. We have determined the content of sugars, organic acids, vitamin C, glutathione and phenolics in apple flesh, with the addition of phenolic content in apple peel and leaves. The change in color in halves and pomace was also measured and the activity of peroxidase (POX) and polyphenol oxidase (PPO) were determined. Additionally, the analyses of flesh were repeated post-storage. The most prominent results were high acidity (malic acid), low phenol content, especially hydroxycinnamic acid and flavan-3-ol content of cultivar 'Majda' in comparison to 'Golden Delicious', and no difference in PPO activity between cultivars. After the overview of the results, we believe that both low phenol content and high reduced glutathione content impact the non-browning characteristics of cultivar 'Majda'.

**Keywords:** non-browning; polyphenol oxidase; phenolics; vitamin C; glutathione
