*3.4. Soluble Cuticular Waxes Content*

Total soluble epi- and intra-cuticular wax content of sweet cherries was determined using two fruits per tree, by the method of [35]. Two fruits without peduncle per tree were weighed and immersed in a mix of chloroform:methanol (3:1) for 2 min at 25 ◦C. After that, the fruits were discarded and the solution was filtered and placed in a glass previously identified and weighed. After about a week, when the glasses were dry, they were weighted again. The weight of the fruits and the difference in the weight of the glasses were used to calculate the wax content, which was expressed as µg g−<sup>1</sup> of fresh weight.

#### *3.5. Fruit Weight and Size*

Fruit weight and size were determined for the 30 fruits of the second sub batch. Fruit weight (g) was evaluated using an electronic balance (EW2200-2NM, Kern, Germany) and fruit size was determined by measuring the width (mm) using electronic calipers (500-196-30, Mitutoyo, UK).
