*2.1. DNA Vaccines*

Latent protein vaccine consensus sequences for EBNA1vax, LMP1vax, and LMP2Avax were produced from sequences obtained from strains AG876, B95-8, and GD1. Codons corresponding to residues associated with cell signaling were modified. Repetitive sequences were deleted from the EBNA1vax consensus sequence to avoid their inhibition of translation and MHC class I presentation [30–32], and alanine mutations were made, affecting binding to USP7 [33]. Similarly, mutations were made to functional domains of LMP1vax and LMP2Avax to avoid signaling through potentially oncogenic pathways [34–39]. The sequences were codon optimized using SynCon technology and prepared for vaccination studies within modified pVAX1 plasmids, as previously described [40].
