**Appendix A**

**Figure A1.** In vitro stability of BCG.HTI2auxoint. *In vitro* stability was assessed by PCR detection of the HTI gene in the BCG.HTI2auxo.int master seeds after five weekly subcultures with and without added lysine; lane 1; BCG.HTI2auxo.int clone 1; lane 2: BCG.HTI2auxo.int clone 3; lane 3: BCG.HTI2auxo.int clone 1 cultured in lysine supplemented medium; lane 4: BCG.HTI2auxo.int clone 3 cultured in lysine supplemented medium; lane 5: BCG.Ø2auxo.int; lane 6: distilled water; lane 7: p2auxo.HTI.int plasmid; lane 8: molecular weight marker.
