**4. Conclusions**

Our original developed TEpredict/PolyCTLDesigner software was used in the study to predict cytotoxic and T-helper epitopes in a compound of seven Ebola virus proteins (GP, VP24, VP30, VP35, L, VP40, and NP) and to design two polyepitope immunogens EV.CTL and EV.Th on the base of those epitopes. Recombinant plasmids, candidate DNA vaccines against Ebola virus encoding the designed antigens, were obtained. We show that the designed DNA vaccine constructs provide a synthesis of corresponding mRNA and proteins in a eukaryotic cell culture, as well as induce statistically significant responses both of CD4+ and CD8+ T-lymphocytes in immunized animals, and consequently are promising candidates for further studies of their capacity to induce cytotoxic and protective responses.

**Author Contributions:** Conceptualization, S.I.B., A.A.I. and D.V.A.; Methodology, S.I.B., L.I.K. and D.V.A.; software, D.V.A.; validation, S.I.B., L.I.K., O.N.K. and D.V.A.; Formal analysis, D.V.A.; Investigation, S.F.O., O.N.K., E.V.S., S.G.D. and S.A.F.; data curation, S.I.B. and D.V.A.; Writing—Original Draft preparation, S.I.B. and D.V.A.; Writing—Review and Editing all authors; visualization, S.I.B., L.I.K. and D.V.A.; supervision, A.A.I. and S.I.B.

**Funding:** The study was funded by GZ-25/16 state assignment.

**Conflicts of Interest:** The authors declare no conflict of interest, financial or otherwise.
