*2.14. Phagocytic Activity*

Phagocytic activity was determined by quantification of reactive oxygen species (ROS) production by the luminol-dependent chemiluminescence (CL) method. As a CL activator, we used opsonized zymosan, as described previously [44]. For analysis, we used a suspension of mice splenocytes and peripheral blood leukocytes from 10 immunized mice of each group. Spontaneous and induced CL were measured on a Synergy H1 hybrid multifunction photometer (BioTek, USA). As a quantitative indicator of the level of ROS production, we used an activation index (AI) – the ratio of the intensity of induced CL to the intensity of spontaneous CL.
