*2.6. Cell Lines*

Retroviruses encoding B95-8 LMP2A and a green fluorescent protein (GFP) reporter were produced by transfecting Phoenix cells (ATCC) with LMP2A sequence in pBMN-I-GFP. The retrovirus-containing media harvested from these cells was used to infect TC-1 cells by spin-infection to generate a tumor cell line, as previously described [45], which stably expresses LMP2A. Cells expressing the GFP marker were isolated using FACS, and single-cell cloning was performed to obtain a clonal cell population.
