*3.4. Designing Artificial Genes and Producing Recombinant Plasmids—Candidate DNA Vaccines Against Ebola Virus Encoding Polyepitope Immunogens of Ebola Virus*

Artificial genes encoding EV.CTL and EV.Th-immunogens of Ebola virus were designed using GeneDesigner software [27]. Reverse translation of amino acid sequences was conducted considering the frequency of codons in humans [55]. Kozak sequence (CCGCCACC) is located ahead of ATG initiating codon. At the end of the encoding sequence, three stop-codons (TAGTGATGA) were added. Designed genes—*EV.CTL* and *EV.Th* were synthesized and cloned in pcDNA 3.1 vector plasmid. As the result, we constructed two recombinant plasmids pEV.CTL and pEV.Th—candidate DNA vaccines against Ebola virus.
