*2.1. Software*

Selection of known T-cell epitopes of Ebola viruses was carried out based on the IEDB—Immune Epitope Database (http://iedb.org) [24]. Prediction of T-cell epitopes was conducted using TEpredict software [25]. Design of polyepitope antigens was performed with PolyCTLDesigner [26]. Genes encoding target immunogens were developed using GeneDesigner software [27]; a compound of codons was optimized to achieve high expression of genes in human cells. Analysis of amino acid sequences of peptides, evaluating their conservatism, statistical analysis of obtained findings, and graph plotting were executed in statistical analysis environment R (version 3.2; https://www.R-project.org/, Vienna, Austria) [28].
