*2.8. Data Analysis and Statistics*

Data was analyzed while using Prism v5.0d (GraphPad Inc.La Jolla, CA, USA). Confidence levels (95.0%) and the differences between the groups and doses of vaccines (nonparametric Mann–Whitney U test) (Table 2) were calculated using Prism. A significant difference was considered when a *p*-value of <0.05 was obtained. Inter-vaccination group ratio comparisons of IgG isotypes were made using unpaired two-tailed, student *t* test. IgG isotype ratio comparisons within each vaccination group were made while using Pearsons correlation coefficient r.



parenthesis show OD range.
