*2.6. Multiplex PCR Assay for M. bovis BCG Substrain Pasteur Identification*

The multiplex PCR assay was performed as described previously by Bedwell *et al.* [35], using 5 μL of mycobacterial DNA isolated from BCG.HTI2auxo.int, BCG wt (Pasteur strain, kindly provided by W.R. Jacobs Jr., B.R. Bloom, and T. Hsu (Albert Einstein College of Medicine, New York, NY, USA)), and commercial BCG strains as template in a final reaction volume of 50 μL.
