*2.7. Animal Studies*

Female, 5-7-week-old C57BL/6 and BALB/c mice were purchased from Jackson Labs, and CD-1 mice were purchased from Charles River. The Wistar Institute Institutional Care and Use Committee approved all animal studies under protocol 112762.

Tumors were generated by injecting 2 million TC-1-LMP2A cells into the axillary region, with monitoring of tumor size thereafter. Tumor sizes were measured by taking their longest dimension as length and the perpendicular as width, with tumor volume being calculated using <sup>1</sup> <sup>2</sup> × length × width2. Multifocal tumors were separately measured, and their total volume was calculated as the sum of the individual volumes. Vaccinations introduced 25 μg of DNA delivered within 30 μL of deionized water by intramuscular injection into the tibialis anterior and were followed by EP with the Cellectra 3P device (Inovio Pharmaceuticals) under general anesthesia using inhaled isoflurane, as previously described [46,47]. Blood was collected through submandibular bleeding or post-mortem cardiac punctures.
