*2.3. Immunofluorescence*

Cover slides coated in poly-L-lysine had 293T cells grow on them in 12-well plates and they were transfected with pVAX empty vector, EBNA1vax, LMP1vax, or LMP2Avax DNA vaccine plasmids using Lipofectamine 2000 per the manufacturer's protocol (Invitrogen, Carlsbad, CA, USA). After incubating for two days, the cells were washed with phosphate-buffered saline (PBS), fixed with 4% paraformaldehyde, and permeabilized using Triton X-100 in PBS, as previously described [42]. Commercial antibodies to EBNA1, LMP1, and LMP2A were used for primary staining as above and Invitrogen anti-mouse, anti-rat, and anti-goat secondary antibodies conjugated to AF488, AF647, and APC were used. Slides were imaged using a Leica TCS SP5 Confocal Laser Scanning Microscope and analyzed with Leica LAS AF software (Leica Microsystems, Wetzlar, Germany).
