**Appendix A**

**Figure A1.** EBNA1vax contains deleted regions of glycine-arginine and glycine-alanine repeats marked by Δ (12 and 310 amino acid deletions) and has its DNA-binding domain highlighted. LMP1vax and LMP2Avax have their cytoplasmic, extracellular, and transmembrane regions indicated. Modifications to engineer LMP2Avax derivatives are labeled as well.

**Figure A2.** Engineered LMP1 vaccines enhance cellular immunity. The LMP1 antigen was truncated at the N-terminal, received an IgE leader sequence, and had tetanus toxoid added to its sequence. Splenocytes were stimulated with the 5 strongest MHC class I peptides to LMP1, as predicted in silico, after C57BL/6 mice were vaccinated. (**A**) IFNγ ELISPOT results showing an average of 59 sfu for LMP1tt30. (**B**) Flow cytometry data showing improved CD8 responses following plasmid engineering.
