*2.4. ELISPOT*

Mouse splenocytes were incubated for 24 hours with peptide pools composed of 15mers overlapping by 11 amino acids and covering the full EBNA1, LMP1, and LMP2A proteins (PepTivator EBV, Miltenyi Biotec, Bergisch Gladbach, Germany). Peptides were resuspended at 5 μg/mL during stimulation. IFNγ ELISPOT was performed according to the manufacturer's instructions. Spots were counted using a Cellular Technology Limited ImmunoSpot Analyzer, as previously described [43].
