*2.2. Gene Synthesis and Cloning*

Designed genes were synthesized (CJSC Eurogen, Moscow, Russia) and then cloned into pcDNA3.1 eukaryotic plasmid vector and sequenced. The obtained recombinant plasmids pEV.CTL and pEV.Th—candidate DNA vaccines against Ebola virus were used to prove the expression of designed target genes in eukaryotic cells and to assess their immunogenicity in mice of the BALB/c line.
