*Calliblepharis jubata* **Cultivation Potential—A Comparative Study between Controlled and Semi-Controlled Aquaculture**

**Glacio Souza Araujo <sup>1</sup> , João Cotas <sup>2</sup> , Tiago Morais <sup>3</sup> , Adriana Leandro <sup>2</sup> , Sara García-Poza <sup>2</sup> , Ana M. M. Gonçalves 2,4 and Leonel Pereira 2,\***


Received: 29 September 2020; Accepted: 20 October 2020; Published: 27 October 2020

**Abstract:** *Calliblepharis jubata* is an edible red seaweed and a carrageenan primary producer, considered native in Figueira da Foz (Portugal). *C. jubata* has the particularity of producing only one kind of carrageenan, the iota fraction. However, this seaweed is not yet valuable for the food industry or even for human consumption. In this work, we characterize important biochemical compounds of *C. jubata* growing up within different cultivation techniques and wild specimens. The aim of this work is to know if there are differences between the biological compounds of interest and identify the advantages for human consumption and the food industry. The results supported the nutritional value of the seaweed, where the ones from inshore cultivation (T) were more identical to the wild specimens (F), than the indoor *C. jubata* (A, B, C). The parameters analyzed were fatty acids, carbohydrates and carrageenan content.

**Keywords:** *Calliblepharis jubata*; aquaculture; fatty acids; carbohydrates; carrageenan

## **1. Introduction**

Several eastern countries traditionally consume seaweed as food, mainly Japan, China and Korea [1], due to its high nutritional value as a source of proteins, carbohydrates, vitamins and minerals [2,3]. The great economic interest is also justified by the growing demand for phycocolloid for different uses in the pharmaceutical, food and cosmetics industries [4–9], which has led several countries to cultivate seaweed [10]. The main hydrocolloids of commercial interest in marine algae (agar, carrageenan and alginate) are also known to have several biological activities, such as anticoagulant [11], antithrombotic [12], antiviral [13], immunostimulants [14], thus having great biotechnological applicability [15]. The variety of seaweed commercial applications discussed raises an important sustainable question, which cannot be taken lightly further. In order to sustain a steady supply, a solution that allows a minimal interaction with marine ecosystems is needed. Aquaculture can be that solution [16,17]. Food safety can only be achieved with a feasible and controllable food source. The seaweed aquaculture is considered to be one of the major hypothesis to be part of the solution to obtain global food security [5]. However to be part of the solution, the potential of seaweeds needs to be characterized and, then selected the seaweeds that are simple to be cultivated, without major

problems. And also, the selected seaweeds need to have a good nutritional value as demonstrated by Leandro et al. [5].

Seaweed aquaculture is, above all, a production method which can be scaled up to very high proportions, maintaining several advantages [16,18]. Thus, aquaculture technologies are considered a sustainable alternative to optimizing outputs (quantity and quality) and reducing costs and pollution. These technologies and methods need to be adapted to the environmental request from the aquaculture location and species cultivated, because the aquaculture can be offshore or onshore and abiotic and biotic factors that influence the aquaculture system are very different, which will have a high influence in the aquaculture productivity [19].

Inland saline aquaculture is defined as land-based aquaculture using saline or saltpans groundwater. Inland saline water can differ in chemistry compared with coastal seawater and adjusting the chemistry or choosing species that are tolerant to the differences is one of the major challenges for expansion of inland saline aquaculture. For seaweed aquaculture, a sure water supply and disposal through terminal evaporation ponds provide critical and expensive infrastructure [20]. Therefore, inland saline aquaculture encompasses a number of culture species, systems and water types. The potential for expansion of these production systems is almost unlimited. With the increasing demands on potable water and marine coastal locations, use (or re-use) of inland saline waters provides a critical resource for high-quality seafood production using unproductive or even detrimental, resources. Integration of seaweed aquaculture in conventional fish farms, halophytic culture, would further increase the efficiency and sustainability of these food production systems [20].

All in all, basic and applied research into practical management systems for these systems is rapidly providing us with the knowledge of how to turn these into profitable farming ventures and novel food production methods are needed to further improve global wellbeing and inland saline aquaculture is bound to be a most valuable tool [20].

The future development work of aquaculture techniques, both in shore and off shore, has to account for studies into those subjects and relate those with designs prepared for strong water movement and rich inorganic nutrient concentrations in order to enhance nutrient uptake (off shore aquaculture) or with aeration, dividers for agitation and CO<sup>2</sup> adjustments for pH control (in shore aquaculture).

The benthic seaweed *Calliblepharis jubata* [21] (Figure 1), which belongs to Class Rhodophyta, Order Gigartinales and Family Cystocloniaceae, is a candidate with an interesting profile for cultivation and extraction of phycocolloids. It presents a dark reddish-brown, cartilaginous but flaccid stem, up to 30 cm in length. Stipe ± cylindrical, up to 10 cm in length; slightly branched rhizoid. Lanceolate blade, up to 15 cm wide, simple, irregularly pinned or dichotomously divided, coated by rolled or hook-shaped proliferations, similar to tendons up to 10 cm or more; blade surface with small spiny growths in well-developed specimens. They have rocks as habitat or are epiphyte, from the medium horizon of the intertidal level to the shallow infralittoral [22].

The studies in this seaweed are mainly about their polysaccharide content. Zinoun et al. [23] reported sulfohydrolase catalyze reaction in this species, which converts carrageenan precursors molecules (µ- and ν-carrabiose) into 3,6 anhydrogalactose derivatives modifying the non-gelling precursors characteristic into gelling carrageenans derivatives, such iota-carrageenan [24]. Deslandes et al. [25] recurred to spectroscopic techniques to elucidate about the monosacharides composition of four red seaweeds, which demonstrated that the main precursor of carrageenan in *C. jubata* is the ν-carrabiose. This seaweed carrageenan has been characterized by vibrational spectroscopy (FTIR-Raman and FITR-ATR), that gives a hybrid iota or iota-kappa carrageenan, with very low content of kappa-carrageenan, in the all *C. jubata* life cycle phase [26].

*Appl. Sci.* **2020**, *10*, x FOR PEER REVIEW 3 of 12

**Figure 1.** *Calliblepharis jubata*. **Figure 1.** *Calliblepharis jubata*.

The studies in this seaweed are mainly about their polysaccharide content. Zinoun et al. [23] reported sulfohydrolase catalyze reaction in this species, which converts carrageenan precursors molecules (µ- and ν-carrabiose) into 3,6 anhydrogalactose derivatives modifying the non-gelling Thus, *C. jubata* is an iota-carrageenan primary producer and is considered native in Figueira da Foz (Coimbra, Portugal). However, this seaweed is not yet valuable for the food industry or even for human consumption [27].

precursors characteristic into gelling carrageenans derivatives, such iota-carrageenan [24]. Deslandes et al. [25] recurred to spectroscopic techniques to elucidate about the monosacharides composition of four red seaweeds, which demonstrated that the main precursor of carrageenan in *C.jubata* is the νcarrabiose. This seaweed carrageenan has been characterized by vibrational spectroscopy (FTIR-Raman and FITR-ATR), that gives a hybrid iota or iota-kappa carrageenan, with very low content of kappa-carrageenan, in the all *C. jubata* life cycle phase [26]. In this work, we characterize important biochemical compounds (fatty acids, carbohydrates) and yield of an important compound (carrageenan) in wild specimens of *C. jubata* and, furthermore with different cultivation techniques to confirm if this species can be cultivated and be a new source of an important compound in the food industry and also, in human direct consumption. Still, there is a lack of information in literature about this species, with a single article of Zinoun and Cosson [28] supporting the possibility of *C. jubata*'s cultivation.

#### Thus, *C. jubata* is an iota-carrageenan primary producer and is considered native in Figueira da Foz (Coimbra, Portugal). However, this seaweed is not yet valuable for the food industry or even for **2. Materials and Methods**

#### human consumption [27]. *2.1. Reagents*

In this work, we characterize important biochemical compounds (fatty acids, carbohydrates) and yield of an important compound (carrageenan) in wild specimens of *C. jubata* and, furthermore with different cultivation techniques to confirm if this species can be cultivated and be a new source of an important compound in the food industry and also, in human direct consumption. Still, there is a Methanol was purchased from José Manuel Gomes dos Santos, Lda., Odivelas, Portugal and acetone from the Ceamed Lda., Funchal, Portugal. Ethanol was obtained from Valente e Ribeiro. Lda., Belas, Portugal and sodium hydroxide from Sigma-Aldrich GmbH, Steinheim, Germany.

#### lack of information in literature about this species, with a single article of Zinoun and Cosson [28] *2.2. Seaweed Collection*

supporting the possibility of *C. jubata*'s cultivation. **2. Materials and Methods**  *2.1. Reagents*  Methanol was purchased from José Manuel Gomes dos Santos, Lda., Odivelas, Portugal and acetone from the Ceamed Lda., Funchal, Portugal. Ethanol was obtained from Valente e Ribeiro. Lda., Belas, Portugal and sodium hydroxide from Sigma-Aldrich GmbH, Steinheim, Germany. The specimens of *Calliblepharis jubata* were collected in Praia da Tamargueira, Buarcos, Figueira da Foz (40◦10018.600 N, 8◦53044.400 W), Portugal (Figure 2). Sampling was conducted in May 2020 from the sites with well-established *C. jubata* patches and without epiphytes or degradation visible at eyesight. The *C. jubata* specimens were collected in two different tidal pools. The pools distance was proximate 1 m between them horizontally to guarantee the most identical composition and physical state between the seaweed collected for analysis and cultivation. Once harvested, samples were stored in plastic bags for transport to the laboratory, in a cool box. The samples were then transported in cold boxes to the laboratory, where they were washed with filtered seawater in order to remove sands, contaminants and other organisms that they may have had. After that, the samples were separated:

*2.2. Seaweed Collection* 

some specimens were weighted and placed in culture systems, while the remaining biomass was rapidly washed with distilled water, cleaned with paper and stored at −20 ◦C for further analysis. weighted and placed in culture systems, while the remaining biomass was rapidly washed with distilled water, cleaned with paper and stored at −20 °C for further analysis.

other organisms that they may have had. After that, the samples were separated: some specimens were

*Appl. Sci.* **2020**, *10*, x FOR PEER REVIEW 4 of 12

The specimens of *Calliblepharis jubata* were collected in Praia da Tamargueira, Buarcos, Figueira da Foz (40°10′18.6′′ N, 8°53′44.4′′ W), Portugal (Figure 2). Sampling was conducted in May 2020 from the sites with well-established *C. jubata* patches and without epiphytes or degradation visible at eyesight. The *C. jubata* specimens were collected in two different tidal pools. The pools distance was proximate 1 m between them horizontally to guarantee the most identical composition and physical state between the seaweed collected for analysis and cultivation. Once harvested, samples were stored in plastic bags

**Figure 2.** Sampling site: Praia da Tamargueira, Buarcos, Figueira da Foz, Coimbra, Portugal. **Figure 2.** Sampling site: Praia da Tamargueira, Buarcos, Figueira da Foz, Coimbra, Portugal.

#### *2.3. Seaweed Cultivation 2.3. Seaweed Cultivation*

Juveniles' specimens were selected during the primary seaweed collection and transported to the laboratory in plastic bags containing seawater and packed in cool boxes. Seawater was also collected to help in the specimens washing process, as well as to be the medium of the indoor cultivation first stage of the *C. jubata*, after filtering with paper filters. Juveniles' specimens were selected during the primary seaweed collection and transported to the laboratory in plastic bags containing seawater and packed in cool boxes. Seawater was also collected to help in the specimens washing process, as well as to be the medium of the indoor cultivation first stage of the *C. jubata*, after filtering with paper filters.

#### 2.3.1. Indoor Cultivation

2.3.1. Indoor Cultivation Most of the juveniles used for cultivation (<5 cm) were selected for the indoor cultivation under controlled conditions at the Marine Algae Laboratory (MARE UC), Department of Life Sciences, Most of the juveniles used for cultivation (<5 cm) were selected for the indoor cultivation under controlled conditions at the Marine Algae Laboratory (MARE UC), Department of Life Sciences, Faculty of Science and Technology, University of Coimbra, Coimbra, Portugal.

Faculty of Science and Technology, University of Coimbra, Coimbra, Portugal. The cultivation apparatus were round volumetric flasks of 1 L, containing 1 L of filtered seawater, with artificial light and artificial aeration from a diaphragm pump, 24 h per day of light. The light was on during periods of 16 h (day period 16 hL:8 hD), the light bar was at a distance of 10 The cultivation apparatus were round volumetric flasks of 1 L, containing 1 L of filtered seawater, with artificial light and artificial aeration from a diaphragm pump, 24 h per day of light. The light was on during periods of 16 h (day period 16 h<sup>L</sup> :8 hD), the light bar was at a distance of 10 cm of the flasks horizontally, the light was an OSRAM light tube with 18 W, 3100 LM and 4000 k.

cm of the flasks horizontally, the light was an OSRAM light tube with 18 W, 3100 LM and 4000 k. The cultivation was carried out in triplicate, with average initial weights of 1.75 g ± 0.59 of *C. jubata* per liter of seawater, for a period of 42 days. The room temperature was 24 °C ± 2 °C and 55% ± 5% humidity. Every two days, the water in each container was renewed three times a week. Once The cultivation was carried out in triplicate, with average initial weights of 1.75 g ± 0.59 of *C. jubata* per liter of seawater, for a period of 42 days. The room temperature was 24 ◦C ± 2 ◦C and 55% ± 5% humidity. Every two days, the water in each container was renewed three times a week. Once a week, the algae were weighed on a semi-analytical balance to monitor their average growth.

a week, the algae were weighed on a semi-analytical balance to monitor their average growth.

## 2.3.2. Inshore Cultivation

In order to obtain more information, we recurred during the MENU—Marine macroalgaE—alternative recipes for a daily Nutritional diet project (Project Reference: FA\_05\_2017\_011) to a one prototype cultivation tank from Lusalgae Lda. (Figueira da Foz, Coimbra, Portugal), to check the potential of *C. jubata* in the method applied in this seaweed aquaculture company. To obtain sufficient biomass for the extraction of compounds of biological interest and to compare them with seaweed grown under controlled conditions, in this stage, *C. jubata* grew in a water tank exposed to direct sunlight with aeration during the day (the aeration did not work during the night, only worked in the sunlight).

The specimens cultivated where identical to the Indoor cultivation (collected in the same pools in the same collection date, with length lower than 5 cm), to prevent the biochemical composition differential due to the seaweed growth state. With this, the cultivation method was the principal factor of differentiation of the biochemical profile.

The medium of cultivation was estuarine seawater (29%–34%) of the Mondego River estuary, in Figueira da Foz, Coimbra, Portugal, without adding nutrients.

The cultivation tank had a capacity of 1000 L, containing 800 L of mechanically filtered estuarine water.

The cultivation started from an initial biomass of 666 g in a single tank. Approximately 75% of the water volume was renewed three times a week and after three weeks the entire biomass was retired to further analysis. The seaweed cultivation method was done by the Lusalgae cultivation standardized method to the red seaweeds.

#### *2.4. Seaweed Treatment before the Biochemical Characterization*

The specimens were washed briefly with distilled water to remove salts and dried in an air force oven (Raypa DAF-135, R. Espinar S.L., Barcelona, Spain) at 60 ◦C, 48 h. The dried algae were finely ground with a commercial mill (Taurus aromatic, Oliana, Spain) (≤1 mm) in order to render the samples uniform and then, stored in a dark room, in a box with silica gel to reduce the humidity, at ambient temperature (±24 ◦C). The dried biomass was stored to protect it from the light, until the analysis proceeds.

#### 2.4.1. Fatty Acids Characterization

Samples were divided into three replicates and were processed in order to obtain the extracts. The lipids were extracted and methylated to fatty acids methyl esters (FAMEs) following the methodology described in Gonçalves et al. [29]. Samples were incubated with methanol for the extraction of lipids. The internal standard nonadecanoic acid C19 (Fluka 74208) was added to each sample to quantify the fatty acid (FA). Then Sodium chloride was added and the samples were centrifuged to the separation of FAMEs. Then, samples were centrifuged and stored at −80 ◦C. FAMEs identification was carried out through Gas chromatography-Mass spectrometry (GC-MS), equipped with a 0.32 mm internal diameter, 0.25 µm film thickness and 30 m long TR-FFAP column. The sample (1.00 µL) was injected with a splitless mode. The column temperature was programmed to increase from 80 to 230 ◦C, with helium as the carrier gas, at a flow rate of 1.4 mL min−<sup>1</sup> . Integration of FAMEs peaks was carried out using the equipment's software. The identification of each peak was performed by retention time and mass spectrum of each FAME, comparing to the Supelco®37 component FAME mix (Sigma-Aldrich, Steinheim, Germany). Quantification of FAMEs was done as previously described in Gonçalves et al. [29].

#### 2.4.2. Carbohydrates Content Characterization

The profiles of three replicates were extracted throughout a hydrolysis, followed by reduction and acetylation [30]. Samples were incubated with sulphuric acid and then added distilled water. After a reduction with ammonia and sodium borohydride, acetylation was performed with acid acetic, methylimidazole and acetic acid anhydride as described in Coimbra et al. [22]. The internal standard 2-desoxiglucose was added to the quantification of sugars. Samples were centrifuged and resuspended in acetone to be injected in the Gas-chromatography with a flame ionization detector (GC-FID). The samples were run through a Thermo Scientific Trace 1310 chromatography equipment (Waltham, MA, USA) equipped with a GC-FID. A TG-WAXMS A (30 m length, 0.32 mm i.d., 0.25 µm film thickness) GC column was used and the oven was programmed to an initial temperature of 180 ◦C, following a linear temperature increase of 5 ◦C min−<sup>1</sup> until the final temperature of 230 ◦C was reached, maintaining this temperature for 12 min. The carrier gas was helium at a flow rate of 2.5 mL min−<sup>1</sup> . The monosaccharides were identified by retention time comparison with standards. Quantification

of sugars was performed by comparison of the sugar chromatographic peaks to the peaks obtained for the internal standard used (2-desoxiglucose).

#### *2.5. Carrageenan Refined Alkali Extraction*

Alkali extraction was performed according to the method described by Pereira et al. [31]. The milled seaweed was weighted in a scale (Radwag WLC 1/A2, Radwag, Radom, Poland) and 1 g samples was used (*n* = 3). Before extraction, the milled seaweed material (1 g) was resuspended and pretreated with an acetone: methanol (1:1) solution in a final concentration of 1% (*m*/*v*) for 16 h, at 4 ◦C, to eliminate the organic-soluble fraction. The liquid solution was decanted, and the seaweed residues obtained were dried in an air force oven (Raypa DAF-135, R. Espinar S.L., Barcelona, Spain) at 60 ◦C before the extraction method.

The samples were placed in 150 mL of NaOH (1 M) (1 g of initial seaweed: 150 mL of NaOH solution) in a hot water bath system (GFL 1003, GFL, Burgwedel, Germany), at 85–90 ◦C, for 3 h. The solutions were hot filtered, under vacuum, through a cloth filter supported in a Buchner funnel and a Kitasato flask. After that, the extracts were again filtered under vacuum with a Goosh 2 silica funnel. The extract was evaporated (rotary evaporator model: 2600000, Witeg, Germany) under vacuum to one-third of the initial volume. The carrageenan was precipitated by adding the warm solution to twice its volume of 96% ethanol. The carrageenan precipitated was washed with ethanol, 48 h at 4 ◦C before drying in an air force oven.

#### *2.6. Statistical Analysis*

For the characterization assays, the statistical analysis of variance (ANOVA) was performed, one for each component, followed by Tukey's tests in order to compare the values in the quantifications within the treatment (type of cultivation's tank or field samples). To determine whether anyof the differences between the means were statistically significant, the *p*-value was compared to a significance level (denoted as α or alpha) of 0.05. Differences were considered significant when the *p* value was lower than 0.05.

#### **3. Results**

In the two seaweeds cultivation systems, we used identical specimens, collected in the same spots in the same collection date. However, at the end of the cultivation of *C. Jubata* under controlled conditions (42 days), a final average weight of 4.25 ± 1.09 g was obtained (Table 1). In the inshore aquaculture the initial biomass only grew 100 g, performing 766 g after three weeks.


**Table 1.** Average weight of *C. jubata* macroalgae during cultivation under controlled conditions. The values indicate the average weight of the macro algae ± standard deviation.

#### *3.1. Fatty Acids Characterization*

Field and Tank cultivated samples were richer in total fatty acids than the cultivated samples under the controlled laboratory conditions. Table 2 shows the Tank T was richer in saturated fatty acids (SFA, 2186.69 µg·g <sup>−</sup><sup>1</sup> dw), while aquarium C was the poorest (1257.64 <sup>µ</sup>g·<sup>g</sup> <sup>−</sup><sup>1</sup> dw). Furthermore, tank T was also the highest in monounsaturated fatty acids (MUFA, 305.74 µg·g <sup>−</sup><sup>1</sup> dw) and polyunsaturated fatty

*3.2. Carbohydrate Characterization* 

acids (PUFA, 137.96 µg·g <sup>−</sup><sup>1</sup> dw) concentration respectively, whereas aquarium B presented the lowest concentration on MUFA (266.03 µg·g <sup>−</sup><sup>1</sup> dw) and PUFA (20.79 <sup>µ</sup>g·<sup>g</sup> <sup>−</sup><sup>1</sup> dw). However, the samples of the field had the highest concentration of highly unsaturated fatty acids (HUFA, 2602.41 µg·g −1 dw) and the lowest was aquarium B (506.73 µg·g <sup>−</sup><sup>1</sup> dw). Referring to the total fatty acid composition, field samples presented the highest value (5289.05 µg·g <sup>−</sup><sup>1</sup> dw) while aquarium B showed the lowest (2105.43 µg·g −1 *Appl. Sci.*  dw) content (Figure 3). **2020**, *10*, x FOR PEER REVIEW 8 of 12

**Figure 3.** Sum of fatty acid group and total fatty acid expressed in µg.g−1 dw from samples of *C. jubata* obtained from cultivated in indoor balloons (A, B and C), cultivated in outdoor tank (T) and collected from the field (F). The same upper letters (a, b, c) mean there are no differences between the treatments (cultivation type). Data as mean value, distribution and variance; n = 3. **Figure 3.** Sum of fatty acid group and total fatty acid expressed in µg·g <sup>−</sup><sup>1</sup> dw from samples of *C. jubata* obtained from cultivated in indoor balloons (A, B and C), cultivated in outdoor tank (T) and collected from the field (F). The same upper letters (a, b, c) mean there are no differences between the treatments (cultivation type). Data as mean value, distribution and variance; *n* = 3.

Monosaccharides present in the samples were analysed after hydrolysis of the polysaccharides. Our method does not permit to separate the two hexoses, glucose and galactose, so they appear together and the results are showing the total glucose + galactose. These two residues (sum of glucose and galactose) were highly identified in all samples, followed by mannose and xylose (Figure 4).

**Figure 4.** Total monosaccharide composition expressed in mg.g<sup>−</sup>1 dw from samples of *C. jubata* obtained from cultivated in indoor balloons (A, B and C), cultivated in outdoor tank (T) and collected

*Appl. Sci.* **2020**, *10*, 7553

**Table 2.** Fatty acid composition and summary of fatty acid grouped in saturated (SFA), monounsaturated (MUFA), polyunsaturated (PUFA), highly unsaturated (HUFA) and total of fatty acids (FA). Results were expressed in µg·g −1 of dry weight (dw) from samples of *C. jubata* obtained from cultivated in indoor balloons (A, B and C), cultivated in outdoor tank (T) and collected from the field (F). Data as mean ± SEM; *n* = 3.


#### *3.2. Carbohydrate Characterization 3.2. Carbohydrate Characterization*

Monosaccharides present in the samples were analysed after hydrolysis of the polysaccharides. Our method does not permit to separate the two hexoses, glucose and galactose, so they appear together and the results are showing the total glucose + galactose. These two residues (sum of glucose and galactose) were highly identified in all samples, followed by mannose and xylose (Figure 4). Monosaccharides present in the samples were analysed after hydrolysis of the polysaccharides. Our method does not permit to separate the two hexoses, glucose and galactose, so they appear together and the results are showing the total glucose + galactose. These two residues (sum of glucose and galactose) were highly identified in all samples, followed by mannose and xylose (Figure 4).

**Figure 3.** Sum of fatty acid group and total fatty acid expressed in µg.g−1 dw from samples of *C. jubata*

(cultivation type). Data as mean value, distribution and variance; n = 3.

*Appl. Sci.* **2020**, *10*, x FOR PEER REVIEW 8 of 12

**Figure 4.** Total monosaccharide composition expressed in mg.g<sup>−</sup>1 dw from samples of *C. jubata* obtained from cultivated in indoor balloons (A, B and C), cultivated in outdoor tank (T) and collected **Figure 4.** Total monosaccharide composition expressed in mg·g <sup>−</sup><sup>1</sup> dw from samples of *C. jubata* obtained from cultivated in indoor balloons (A, B and C), cultivated in outdoor tank (T) and collected from the field (F). The same upper letters (a, b) mean there are no differences between the treatments (cultivation type). Data as mean value, distribution and variance; *n* = 3. *Appl. Sci.* **2020**, *10*, x FOR PEER REVIEW 9 of 12 from the field (F). The same upper letters (a, b) mean there are no differences between the treatments (cultivation type). Data as mean value, distribution and variance; n = 3.

#### *3.3. Carrageenan Extraction 3.3. Carrageenan Extraction*

**4. Discussion** 

In the extraction of the main polysaccharide, all the samples have differences, with the F sample being the best sample assayed and the tank T cultivation (Outdoor) appearing to be the most stable sample in terms of extraction. In this case, all the indoor cultivations were inserted in only segment (Figure 5). In the extraction of the main polysaccharide, all the samples have differences, with the F sample being the best sample assayed and the tank T cultivation (Outdoor) appearing to be the most stable sample in terms of extraction. In this case, all the indoor cultivations were inserted in only segment (Figure 5).

**Figure 5.** Yield of carrageenan per type of cultivation. a, b, c Equal letters indicate no significant differences at the *p*-value < 0.05 level. **Figure 5.** Yield of carrageenan per type of cultivation. a, b, c Equal letters indicate no significant differences at the *p*-value < 0.05 level.

impact in the semi-controlled cultivation systems, the inshore cultivation system.

conditions in tank T were more similar to the field environmental factors.

content in carrageenan and more content in FAs than the inshore cultivation.

*crispus* and *Neopyropia tenera* [32,33].

*C. Jubata* indoor cultivation has been studied for a long time by the research team, to obtain more information about this seaweed which has an interesting carrageenan, still with scarce or null information in literature. The cultivation in the lab was proven to be feasible to obtain more information about the performance of this seaweed to be cultivated. However, the inshore cultivation was not possible to be done in triplicated due to the lack of available cultivation tanks, although this first assay demonstrated interesting results in all the assays. This happened mainly due to the cultivation location being near the collection site and the water being considered identical by other researchers that monitor the water quality, in various locations near the collection and cultivation sites. However, the growth was poor when compared to indoor cultivation, this can be explained to the abiotic and biotic factors

Samples collected in the field showed the highest value of fatty acids content since they were outdoors, where the conditions were not under control, due to the constant renewal of the water and the nutrients availability. After field samples, the higher value of fatty acid concentration was followed by tank T which was outside and in a larger proportion of cultivated seaweed. Indeed, the

In the carrageenan yield, the indoor culture was so identical in results that we put together to have more robust analysis. With that analysis, we observed that indoor cultivation presented lower

This is the first time that this species and genus is targeted to be characterized in the fatty acids and carbohydrates and there is a general lack of information about this seaweed, Whose cultivation is more feasible in lower temperatures [28]. However, this species is identical with high rated food consumed red seaweeds in the fatty acids and carbohydrates levels, as *Palmaria palmata*, *Chondrus* 

#### **4. Discussion**

*C. Jubata* indoor cultivation has been studied for a long time by the research team, to obtain more information about this seaweed which has an interesting carrageenan, still with scarce or null information in literature. The cultivation in the lab was proven to be feasible to obtain more information about the performance of this seaweed to be cultivated. However, the inshore cultivation was not possible to be done in triplicated due to the lack of available cultivation tanks, although this first assay demonstrated interesting results in all the assays. This happened mainly due to the cultivation location being near the collection site and the water being considered identical by other researchers that monitor the water quality, in various locations near the collection and cultivation sites. However, the growth was poor when compared to indoor cultivation, this can be explained to the abiotic and biotic factors impact in the semi-controlled cultivation systems, the inshore cultivation system.

Samples collected in the field showed the highest value of fatty acids content since they were outdoors, where the conditions were not under control, due to the constant renewal of the water and the nutrients availability. After field samples, the higher value of fatty acid concentration was followed by tank T which was outside and in a larger proportion of cultivated seaweed. Indeed, the conditions in tank T were more similar to the field environmental factors.

In the carrageenan yield, the indoor culture was so identical in results that we put together to have more robust analysis. With that analysis, we observed that indoor cultivation presented lower content in carrageenan and more content in FAs than the inshore cultivation.

This is the first time that this species and genus is targeted to be characterized in the fatty acids and carbohydrates and there is a general lack of information about this seaweed, Whose cultivation is more feasible in lower temperatures [28]. However, this species is identical with high rated food consumed red seaweeds in the fatty acids and carbohydrates levels, as *Palmaria palmata*, *Chondrus crispus* and *Neopyropia tenera* [32,33].

The carrageenan extraction yield from all the samples is identical with the work of Pereira [21] in the same location of seaweed collection, however lower when compared to the work of the Zinoun and Cosson [28], that obtained 29% at the lowest yield of *C. jubata*, in which was collected in Cap Levy, in France.

Information about carbohydrates content is null in the literature, this being the first study characterizing the carbohydrate profile of this species and in *Calliblepharis* genus.

#### **5. Conclusions**

Our results support that seaweed indoor cultivation can help to optimize the overall seaweed cultivation allowing the assess to the ideal growth conditions of *C. Jubata*. However, this controlled cultivation system had a negative effect in the *C. Jubata* biochemical profile. Thus, the inshore cultivation (partially controlled system) showed more similar results with field samples than with the indoor cultivation system. However, the seaweed inshore cultivation needs to be more assayed to have high growth rate without lowering the biochemical compounds. The procedure of cultivation needs to be optimized to achieve and guarantee high content profiles and thus the best nutritional value.

Further and next plans of the study are to optimize and obtain more information about the aquaculture process of this seaweed species and the full characterization of the nutritional value of wild and cultivated *C. jubata* in parallel with the optimization procedure to guarantee to the stakeholders and farmers the best quality cultivation. The biochemical profile needs to be further analyzed in the quantification and characterization of the uronic acids and sulfate compounds, protein content and amino-acids profile, ash quantification and mineral concentration to have a useful tool to promote the *C. Jubata* cultivation.

**Author Contributions:** Conceptualization, G.S.A., J.C. and A.M.M.G.; Seaweed Cultivation, G.S.A., J.C. and T.M.; Fatty Acids and Carbohydrates analysis, A.L. and S.G.-P.; Carrageenan research, G.S.A. and J.C.; writing—original draft preparation, G.S.A., J.C., T.M., A.L. and S.G.-P.; writing—review and editing, J.C., A.M.M.G. and L.P.; supervision, A.M.M.G. and L.P.; project coordination, A.M.M.G.; funding acquisition, A.M.M.G. and L.P. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work is financed by national funds through FCT - Foundation for Science and Technology, I.P., within the scope of the projects UIDB/04292/2020 – MARE - Marine and Environmental Sciences Centre and UIDP/50017/2020+UIDB/50017/2020 (by FCT/MTCES) granted to CESAM - Centre for Environmental and Marine Studies. This work was financed by the Live Food Production Laboratory (LABPAV) and the Tropical Aquaculture Study Group (GEAQUI) of the Federal Institute of Education, Science and Technology of Ceará—IFCE, Campus Aracati, Ceará, Brazil. João Cotas thanks to the European Regional Development Fund through the Interreg Atlantic Area Program, under the project NASPA (EAPA\_451/2016). Adriana Leandro thanks FCT for the financial support provided through the doctoral grant SFRH/BD/143649/2019 funded by National Funds and Community Funds through FSE. Sara García-Poza thanks to the project MENU—Marine Macroalgae: Alternative recipes for a daily nutritional diet (FA\_05\_2017\_011) which co-financed this research, funded by the Blue Fund under Public Notice No. 5—Blue Biotechnology. Ana M. M. Gonçalves acknowledges University of Coimbra for the contract IT057-18-7253.

**Conflicts of Interest:** The authors declare no conflict of interest.

## **References**


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