**5. Conclusions**

We developed a novel mathematical approach to reconstruct functional astrocytic networks based on an analysis of dynamic correlations between calcium events. This approach revealed dramatic changes in the structure and properties of astrocytic networks in normal and ischemia-like conditions.

**Supplementary Materials:** The following are available online at http://www.mdpi.com/1422-0067/21/21/7952/s1, Figure S1: Morphology of primary astrocyte cultures on day 21 of cultivation in vitro, Table S1: Cell viability analysis of primary astrocyte cultures on day 7 after modeled stress factors, Video S1: the propagation of calcium signals between astrocytes.

**Author Contributions:** Conceptualization, E.V.M., M.V.I. and M.V.V.; Data curation, M.V.I.; Formal analysis, M.I.K. and D.E.B.; Investigation, E.V.M., M.I.K., D.E.B., M.O.S. and T.A.M.; Supervision, M.V.I. and M.V.V.; Visualization, M.I.K.; Writing—original draft, E.V.M., M.V.I. and M.V.V. All authors have read and agreed to the published version of the manuscript.

**Funding:** We acknowledge support by the grant of the Ministry of Education and Science of the Russian Federation Agreement No. 075-15-2020-808. This research was carried out using The Core Facilities «Molecular Biology and Neurophysiology».

**Conflicts of Interest:** The authors declare no conflict of interest.
