*4.5. Neuroprotective Protocols*

Neuroprotective drugs were dissolved using dimethyl-sulfoxide (DMSO) (Sigma-Aldrich, St. Louis, MO, USA) [65]. Vehicle controls were performed for OGD and control groups for 0.1 Vol.%, 1.0 Vol.% and 2.0 Vol.% DMSO. For OGD groups, nimodipine was applied at 10 or 20 μM dissolved in either 0.1 Vol.% or 1.0 Vol.% DMSO and in combination with amiloride at 100 μM [27] again at 10 or 20 μM with 1.1 Vol.% or 2.0 Vol.% DMSO, respectively. Finally, we applied amiloride at 100 μM alone with either 1.0 Vol.% or 2.0 Vol.% DMSO. All agents remained within the medium for 72 h after OGD or time control, ending the experiments with the final imaging. An overview of all protective protocols can be seen in Figure 6b and in Appendix A.
