*4.7. Statistical Analysis*

Results were assessed using one-way or two-way analysis of variance (ANOVA), and repeated measures of ANOVA, followed by Tukey's post hoc test, using SPSS 15.0 for Windows, for statistical analysis, for multi-group comparisons, and Student's *t* test for single comparisons, using Prism 5.01 software (Graphpad Software Inc., San Diego, CA, USA). In all statistical assessments, *p* < 0.05 was considered to be statistically significant. Results are expressed as the mean ± standard error of the mean.
