*4.4. Amiloride Administration*

To confirm the effect of amiloride on GCI-induced neuronal death, the experimental groups were divided into four groups: sham (vehicle, amiloride) and global cerebral ischemia (vehicle, amiloride). The amiloride-treated groups were administrated amiloride (10 mg/kg, i.p.) dissolved in 0.9% normal saline. After GCI induction, we immediately injected the amiloride into the intraperitoneal space. The vehicle group was given 0.9% normal saline instead of amiloride. All experimental groups were sacrificed 24 h after GCI.
