*4.5. Ischemia-Like Condition Model*

Ischemia-like conditions were modeled on day 21 of primary astrocyte culture development in vitro by replacing the culture medium with a medium with low oxygen (0.37 mL/L) free of glucose, lactate, and pyruvate for 30 min. The experiment was performed in a sealed chamber in which air was also replaced by argon gas.
