**5. Conclusions**

This present study supports the hypothesis that the administration of amiloride protects against hippocampal neuronal death and zinc accumulation after GCI. In addition, inhibition of NHE-1 by amiloride may have considerable therapeutic potential for the prevention of GCI.

**Author Contributions:** B.S.K. researched and collected the data and reviewed and edited the manuscript. B.Y.C. reviewed and edited the manuscript. A.R.K., S.H.L., D.K.H., J.H.J., D.H.K., and M.K.P. researched and collected the data. S.W.S. contributed to the discussion and wrote, reviewed, and edited the manuscript. All authors have read and agree to the published version of the manuscript.

**Funding:** This study was supported by funding from the National Research Foundation of Korea (NRF) (NRF-2019R1A2C4004912) to B.Y.C. Additionally, this work was supported by the Brain Research Program through the NRF, funded by the Ministry of Science, Information, and Communication Technology and Future Planning (NRF-2017M3C7A1028937 and 2020R1A2C2008480) to S.W.S.

**Conflicts of Interest:** The authors declare no conflict of interest.
