*4.4. Genotype by PCR*

The genotype of each animal was confirmed as described by Carro et al. [80]. Genomic DNA was extracted from the mouse tail using the High Pure PCR template preparation kit (Roche, Barcelona, Spain) according to the manufacturer's instructions. Briefly, 150 ng of DNA was amplified by polymerase chain reaction (PCR) using the specific primers described in [80] to detect mutant human APP sequence.
