2.3.3. Synaptic Proteins

Synaptothysin is associated with synaptic vesicles involved in nerve impulse transmission, and its content in the presynaptic area reflects the functional activity of neurons. The synaptophysin level of fluorescence was reduced in the control NaCl (55.00 [53.00; 61.00]) (Figure 8B,G) and Ad5-GFP (56.00 [48.00; 59.00]) (Figure 8C,G) groups when compared with the intact group (78.00 [61.00; 87.00]) (Figure 8A,G) (*p* < 0.05). Immunoexpression of synaptophysin in the therapeutic Ad5-VEGF-GDNF-NCAM (64.00 [61.00; 72.00]) (Figure 8E,G), UCBC+Ad5-VEGF-GDNF-NCAM (62.00 [61.00; 65.00]) (Figure 8F,G) and UCBC+Ad5-GFP groups (79.00 [76.00; 81.00]) (Figure 8D,G) was higher than in the control groups and did not differ from the intact group (*p* < 0.05).

**Figure 8.** Immunoexpression of Synaptophysin in the rat brain cortex, 21 days after the middle cerebral artery occlusion. Immunofluorescent staining with antibody to synaptic vesicle membrane protein Synaptophysin in intact (**A**), control NaCl (**B**) and Ad5-GFP (**C**), and therapeutic UCB-MC+Ad5-GFP (**D**), Ad5-VEGF-GDNF-NCAM (**E**) and UCB-MC+Ad5-VEGF-GDNF-NCAM (**F**) groups. Arrows indicate Synaptophysin (green glow) in neurons. Arrowheads point to cell nuclei visualized using DAPI (blue glow). Scale bar in (**A**—**F**) = 50 μm; scale bar in the insert = 20 μm. (**G**)—comparative analysis of fluorescence intensity level of Synaptophysin in experimental groups; \*—*p* < 0.05.

Postsynaptic density protein 95 kDa (PSD95) is associated with the functional activity of the postsynaptic membrane, providing its stability and plasticity in interactions with neurotransmitters. As for the intact group, the immune expression of PSD95 (65.0 [62.00; 69.00]) (Figure 9A,G) was reduced in all experimental groups except for the group UCBC+Ad5-VEGF-GDNF-NCAM (55.00 [50.00; 57.00]) (*p* < 0.05) (Figure 9F,G). However, the level of PSD95 fluorescence was significantly higher in the therapeutic Ad5-VEGF-GDNF-NCAM (62.50 [57.75; 67.00]) (Figure 9E,G), UCBC+Ad5-VEGF-GDNF-NCAM and UCBC+Ad5-GFP (50.00 [48.50; 57.50]) (Figure 9D,G) groups, in comparison with the control group NaCl (43.00 [38.50; 46.00]) (Figure 9B,G), which did not differ from the Ad5-GFP group (49.00 [46.00; 51.00]) (Figure 9C,G) (*p* < 0.05).

**Figure 9.** Immunoexpression of PSD95 in the rat brain cortex 21 days after the middle cerebral artery occlusion. Immunofluorescent staining with antibody to postsynaptic membrane protein PSD95 in intact (**A**), control NaCl (**B**) and Ad5-GFP (**C**), and therapeutic UCB-MC+Ad5-GFP (**D**), Ad5-VEGF-GDNF-NCAM (**E**) and UCB-MC+Ad5-VEGF-GDNF-NCAM (**F**) groups. Arrows indicate PSD95 (green glow) in neurons. Arrowheads point to cell nuclei visualized using DAPI (blue glow). Scale bar in (**A**–**F**) = 50 μm; scale bar in the insert = 20 μm. (**G**)—Comparative analysis of fluorescence intensity level of PSD95 in experimental groups; \*—*p* < 0.05.
