**Exercise-Induced Elevated BDNF Level Does Not Prevent Cognitive Impairment Due to Acute Exposure to Moderate Hypoxia in Well-Trained Athletes**

**Zofia Piotrowicz 1,\*, Małgorzata Chalimoniuk 2, Kamila Płoszczyca 3, Miłosz Czuba 3,4 and Józef Langfort <sup>1</sup>**


Received: 16 July 2020; Accepted: 31 July 2020; Published: 4 August 2020

**Abstract:** Exposure to acute hypoxia causes a detrimental effect on the brain which is also manifested by a decrease in the ability to perform psychomotor tasks. Conversely, brain-derived neurotrophic factor (BDNF), whose levels are elevated in response to exercise, is a well-known factor in improving cognitive function. Therefore, the aim of our study was to investigate whether the exercise under hypoxic conditions affects psychomotor performance. For this purpose, 11 healthy young athletes performed a graded cycloergometer exercise test to volitional exhaustion under normoxia and acute mild hypoxia (FiO2 = 14.7%). Before, immediately after exercise and after a period of recovery, choice reaction time (CRT) and number of correct reactions (NCR) in relation to changes in serum BDNF were examined. Additionally, other selected factors which may modify BDNF production, i.e., cortisol (C), nitrite, catecholamines (adrenalin-A, noradrenaline-NA, dopamine-DA, serotonin-5-HT) and endothelin-1 (ET-1), were also measured. Exercise in hypoxic conditions extended CRT by 13.8% (*p* < 0.01) and decreased NCR (by 11.5%) compared to rest (*p* < 0.05). During maximal workload, NCR was lower by 9% in hypoxia compared to normoxia (*p* < 0.05). BDNF increased immediately after exercise in normoxia (by 29.3%; *p* < 0.01), as well as in hypoxia (by 50.0%; *p* < 0.001). There were no differences in BDNF between normoxia and hypoxia. Considering the fact that similar levels of BDNF were seen in both conditions but cognitive performance was suppressed in hypoxia, acute elevation of BDNF did not compensate for hypoxia-induced cognition impairment. Moreover, neither potentially negative effects of C nor positive effects of A, DA and NO on the brain were observed in our study.

**Keywords:** brain-derived neurotrophic factor; moderate hypoxia; physical exercise; psychomotor function; reaction time; cortisol; catecholamines; nitrite; endotheline-1; lactate
