**Appendix B**

**Figure A1.** Example slices for our exclusion criteria. Upper left and middle picture show normal slices at 72 h after OGD or control, respectively. [1.] Image files with more than one slice were excluded. If

the dentate gyrus [2.] or the CA1 region [3.] were not or only unreliably identifiable, slices were excluded. [4.] Dark spots and [7.] inhomogeneous margins are likely due to imprecise preparation technique, while [6.] wrinkling is most likely caused by problems in the culturing process. Lastly, [5.] on a few slices, PI clots were present. Again, slice images are red-channel-filtered and contrast-enhanced. Contrast enhancement does not change grey scale values and is purely performed to ensure a more comfortable visual evaluation of slice images.

The python scripts can be found at https://github.com/jonasort/OGD-analysis-scripts-in-Python. All questions may be asked to the corresponding author.
