*4.7. Biochemical Screening*

To analyze the features of the functional activity of astrocytes, the following agents were added to the culture medium on day 28 of culture development in vitro: ATP (10 μM, Merck KGaA, Darmstadt, Germany) as an activator of calcium activity and Gap19 (100 μM, Merck KGaA, Darmstadt, Germany) to estimate the role of connexin blockade in the implementation of functional calcium reactions. The same volume of PBS was used as a control. The baseline activity was recorded for 10 min.
