*2.1. Dosage-Dependent Reduction in Infarction and Behavior by LE*

The severity of infarction was measured by triphenyltetrazolium chloride (TTC) staining and neurological deficit assessment. The severity of infarction was visible through the unstained areas of the brain in Figure 1a. The Sham+Vehicle (Veh) group did not experience notable infarction. The MCAO+Veh group suffered an injury of approximately 35% of the left hemisphere. The MCAO+LE 10% group exhibited decrease in infarction volume to about 31%, which was not significant compared to MCAO+Veh group (*p* > 0.05, one-way ANOVA). There was a significant decrease in infarction to about 26% in the MCAO+LE 20% group compared to the MCAO+Veh group (*p* < 0.05, one-way ANOVA followed by Tukey's multiple comparison test) (Figure 1b). In behavioral test, all experimental groups suffered a certain degree of behavioral deficit except for the Sham+Veh group. The MCAO+Veh group had an average Bederson score of 3. The MCAO+LE 10% group scored lower in the behavior test and did not differ significantly compared to the MCAO+Veh group (*p* > 0.05, Kruskal-Wallis non-parametric test). The administration of LE 20% significantly decreased the average Bederson score to approximately 2 (*p* < 0.05, Kruskal-Wallis non-parametric test followed by Dunn's post hoc test). The majority of the MCAO+LE 20% group achieved a Bederson score of 2 or under, while the majority MCAO+Veh group recorded between 2 and 4. (Figure 1c).
