4.10.1. Primary Antibodies

The primary antibodies used in this study were: Mouse monoclonal amyloid-β antibody 6E10 (BioLegend Inc., San Diego, CA, USA), diluted 1:1000. Mouse monoclonal anti-HSP70 (1:1000) and rabbit polyclonal antibody against ASC (1:500) were from Santa Cruz (Temecula, CA, USA). Mouse monoclonal anti tau-5 (for measurement of total tau protein) antibody (Chemicon, Madrid, Spain) diluted 1:5000 and rabbit polyclonal to phospho-tau (phospho serine199 + serine202) (1:1000) were from Abcam (Cambridge, UK). Mouse monoclonal anti-glial fibrillar acid protein (GFAP) antibody diluted 1:5000 was from Chemicon (Madrid, Spain) and rabbit polyclonal antibody for anti-Iba1, 1:1000 was for WAKO (Japan). Rabbit anti-proliferating cell nuclear antigen (PCNA, 1:75; SantaCruz Biotechnology, Santa Cruz, CA, USA), and mouse monoclonal anti-ubiquitin antibody (1/500) was from Chemicon (Chemicon International Inc., Temecula, CA, USA). Mouse anti Bcl2 (1:250) and rabbit anti-Bax (1:250) were from Santa Cruz (Santa Cruz Biotechnology). Rabbit anti-phospho-Akt (Ser473P) (1:2000) and rabbit anti-Akt (1:2000) were from Cell Signaling Technology, Beverly, MA, USA. Rabbit anti Nrf2 (1:1000) and rabbit anti CD36 (1:500) were from Thermo Fisher Scientific, Rosemont, IL, USA. To correct and quantify the protein charge mouse monoclonal anti-β-actin antibody diluted 1:10,000 was used from Sigma.
