**Pulsed Ultrasound-Assisted Extraction as an Alternative Method to Conventional Maceration for the Extraction of the Polyphenolic Fraction of** *Ribes nigrum* **Buds: A New Category of Food Supplements Proposed by The FINNOVER Project**

**Federica Turrini <sup>1</sup> , Dario Donno 2,\* , Gabriele Loris Beccaro <sup>2</sup> , Paola Zunin <sup>1</sup> , Anna Pittaluga <sup>1</sup> and Ra**ff**aella Boggia <sup>1</sup>**


Received: 6 September 2019; Accepted: 6 October 2019; Published: 10 October 2019

**Abstract:** In this research, for the first time, an alternative method to produce *Ribes nigrum* bud derivatives is presented. Pulsed ultrasound-assisted extraction (PUAE), using a food-grade solvent according to green chemistry principles, has been employed and compared to the conventional extraction method. Traditionally, bud derivatives, a category of botanicals marketed as plant food supplements in the European Community, are produced by macerating meristematic tissues of trees and plants mainly spontaneously collected. Buds are a challenging raw material for the UAE, since meristematic tissues are much softer and fragile than their corresponding adult phenological stage. It is therefore important to assess whether the polyphenolic fraction, very susceptible to degradation, is conserved after UAE. Untargeted polyphenolic fingerprints (UV-Visible and fluorescence) coupled with chemometrics are employed to quickly screen the best extraction conditions, evaluated by the design of experiment (DoE) method. The polyphenolic fraction of the optimized PUAE extract was quantified by targeted HPLC fingerprint and its antiradical activity was determined. PUAE on a lab pilot reactor was proven to be the most practical approach for a rapid (20 min vs. 21 days maceration) and efficient extraction of bioactive polyphenolics from *Ribes nigrum* buds, encouraging the scale up to an industrial plan.

**Keywords:** phenolic compounds; bud derivatives; *Ribes nigrum* glyceric macerate; green chemistry; pulsed ultrasound-assisted extraction; untargeted spectroscopic fingerprint; targeted chromatographic fingerprint
