*3.12. Cytokine Production*

For quantification of the cytokines IL-6 and TNF-<sup>α</sup>, the supernatant of the cells that were exposed to SPs in the experiment to evaluate ROS levels was used. The cytokines were quantified using the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Kit according to the manufacturer's instructions using a flow cytometer (FACSCanto II, BD Biosciences, OR, USA). The results were recorded with FACSDiva software and analyzed using FCAP Array software, version 3.0 (BD, Franklin Lakes, NJ, USA).
