*4.7. Statistical Analysis*

All measurements were done in three replicates. All results are expressed as mean ± the standard deviation compared by ANOVA. All di fferences were considered to be statistically significant if *p* < 0.05. Data were analyzed using the software Statistic 6.0. (StastSoft, Tulsa, OK, USA). To confirm the normal distribution of variables, Shapiro–Wilks test was performed.
