2.6.2. Antioxidant Capacity

The DPPH radical scavenging capacity method reported by Gao et al. [26] was used to evaluate oil antioxidant capacity (AC). Pecan nut oil (1 mL) was diluted in ethyl acetate (10 mL) and mixed with 2 mL of 0.50 mM DPPH solution. The mixture was vortexed and left to react in darkness for 15 min. After this time, absorbance was measured at 515 nm. A standard curve of Trolox was prepared (0.003–0.030 mg/mL) to express results as mg Trolox equivalents per 100 g of pecan nut oil (mg Trolox EQ/100 g).
