*2.4. Oil Mechanical Extraction*

Oil extraction was performed on freeze-dried kernels (85.0 g) employing a screw-type press (YD-ZY-02A, Yoda Europe, Hangzhou, Zhejiang, China). Pecan nut oil was stored in 50 mL centrifuge tubes flushing N2 into the headspace and the generated cakes were placed in polyethylene bags and vacuum sealed. Oil and cake samples were stored at −40 ◦C until analyses.
