2.5.2. Oil Content

Oil content was analyzed as reported by Villarreal-Lozoya et al. [20]. Samples and hexane (1:10 *w*/*v*) were homogenized (6000 rpm, 1.5 min) using an IKA® T25 ultraturrax (IKA, Staufen im Breisgau, Germany), centrifuged (8500 rpm, 15 min, 20 ◦C) and supernatants collected. This procedure was repeated three times. Hexane was evaporated from pooled supernatants (25 rpm, 45 ◦C) and oil content was determined gravimetrically following the AOAC Official Method 960.39 [19]. Oil content was expressed as g per 100 g of freeze-dried kernels and cakes db (g/100 g db).
