*2.3. Regulated Quality Parameters*

Regulated quality parameters consist of free acidity and peroxide values. To determine free acidity, expressed as % of oleic acid, a solution of melon-seed oil dissolved in ethanol/ether (1:1) was titrated with a 0.1 mol/L potassium hydroxide ethanolic solution [22]. On the other hand, the peroxide value, expressed in milliequivalents of active oxygen per kilogram of oil (meq O2/kg), was measured according to European Union (EU) regulations [22]. Briefly, chloroform and acetic acid were added to an oil sample, mixed vigorously, and left to react with a solution of potassium iodide in the darkness. Then, the free iodine was titrated with a sodium thiosulfate solution [22].
