4.1.5. Effects on Diuresis

The evaluation of the effects of Jaburetox on diuresis was one of the first investigations carried out to understand the mechanism of action of the toxin. In 2009, Stanisçuaski et al. [81] conducted studies on *R. prolixus*' Malpighian tubules to explore, in vitro, the effects of Jaburetox on serotonin-induced diuresis. The authors demonstrated that Jaburetox and also JBU are capable of interacting with membrane factors that end up inhibiting diuresis by triggering different signaling cascades. While JBU effect is mediated by the activation of the eicosanoid cascade and is dependent on Ca++ ions, the antidiuretic effect of Jaburetox is mediated by an increase in cyclic guanosine monophosphate (cGMP) levels. This increment leads to the interruption of ion transport by blockage of the apical V-ATPase and disruption of the transepithelial potential across the tubule's membrane through an unknown pathway, leading (directly or indirectly) to the inhibition of water secretion and consequent impairment of diuresis [81].
