*Article* **Artichoke By-Products as Natural Source of Phenolic Food Ingredient**

**Lucía <sup>L</sup>ópez-Salas 1, Isabel Borrás-Linares 2,\*, David Quintin 3, Presentación García-Gomez 3, Rafael Giménez-Martínez 1, Antonio Segura-Carretero 2,4,† and Jesús Lozano-Sánchez 1,2,†**

	- Correspondence: iborras@cidaf.es; Tel.: +34-958-637-083

**Abstract:** Nowadays, the transformation activity of the food industry results in the generation of a huge amount of daily discarded vegetables wastes. One of those undervalued by-products are produced during the post-harvesting and processing process of artichokes. In the present research, the potential of artichokes' bracts and stalks have been evaluated as a natural source of phenolic compounds which could be used as bioactive food ingredients, among others. In this study, the bioactive composition of those wastes has been evaluated using recent advances in extraction and analytical technologies, concretely, pressurized liquid extraction (PLE) followed by high-performance liquid chromatography (HPLC) coupled to electrospray time-of flight mass spectrometry (ESI-TOF/MS) analysis. To achieve this goal, first, the extraction process was evaluated by a comparative study using GRAS (Generally Recognized As Safe) solvents (mixtures of ethanol and water) at different temperatures (40–200 ◦C). The second step was to deeply characterize the composition of individual polyphenols by HPLC-ESI-TOF/MS in order to establish a comparison among the different PLE conditions applied to extract the phenolic fraction. The analysis revealed a wide variety of phenolic-composition, mainly phenolic acids and flavonoids. The results also highlighted that high percentages of ethanol and medium-high temperatures pointed out to be useful PLE conditions for recovering this kind of phytochemicals, which could be used in different applications, such as functional food ingredients, cosmetics, or nutraceuticals.

**Keywords:** artichoke by-products; phenolic compounds; HPLC-ESI-TOF-MS; PLE; GRAS
