**5. Conclusions**

In the present study, we examined the in vitro antitumoral activities of *V. macrophylla* EO on the SKBR3 human adenocarcinoma cell line. The results demonstrated that this EO is able to induce numerous ultrastructural alterations thanks its ability to both disorganize the cytoskeleton and damage the plasma membrane. Thus, these results sugges<sup>t</sup> a potential use of *V. macrophylla* EO and its main components as a chemosensitizer in clinical practice. Future studies should clarify the mechanism of action and biochemical process activated by this EO. Other studies will be performed using fluorescence microscopy to study all cytoskeletal proteins in order to assess whether these complexes will be used to reduce the degree of invasiveness of human cancer cells by increasing their ability to adhere to the substrate.

**Author Contributions:** Conceptualization: F.M. and A.S.; Figures preparation: M.C. and A.S.; Writing—original draft preparation: F.M., M.C. and A.S.; Analysis of essential oils composition: R.R. and F.M.; Data analysis: F.M., M.C. and A.S. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was partially funded by Istituto Superiore di Sanità, ISS (Ministry of Health-ISS funding) and by University of Camerino.

**Informed Consent Statement:** This study did not involve human.

**Data Availability Statement:** All the data are available.

**Acknowledgments:** We thank Stefania Muran for experimental support in ISS laboratory.

**Conflicts of Interest:** No conflict of interest are present Fondo di Ateneo per la Ricerca (FAR 2014/2015) in this study.
