*2.12. Animals*

Adult Swiss mice (25–30 g) from the Central Animal House of the Federal University of Paraná and adult rabbits (3 kg) from CPPI (Centro de Pesquisa e Produção de Imunobiológicos, Piraquara, Brasil) were used for in vivo experiments with the whole venom of *Loxosceles intermedia* and/or the recombinant toxins (LiRecTCTP, LiRecDT1, and GFP). All procedures involving animals were carried out in accordance with "Brazilian Federal Laws," following the Institutional Ethics Committee for Animal Studies Guidelines from Federal University of Paraná, which approved the project methodology concerning animal studies (Approval Certificates Numbers 730 and 1183).
