*2.13. Growth Curve*

Cells (5 × 104) were seeded in each well of 6-well plate. The cell number for each cell line was counted each day for a 6-day time course; this number has been normalized on cell number counted 24 h after seeding to allow cells to recover from the trypsinization. Cell numbers have been counted in triplicate in three independent experiments. Doubling times were determined by fitting the data points to the exponential growth function in GraphPad Prism 5.01 software.
