*2.20. Statistical Analysis*

All experiments were done at least three times unless otherwise indicated. The results are presented as means ± SD. Results were analysed using a Mann–Whitney test. One-way ANOVA followed by the Bonferroni test using the PRISM GraphPad software was used in the analysis of three or more data sets. Differences were considered significant for *P* < 0.05 and highly significant for *P* < 0.01 and *P* < 0.001
