*2.10. Calcium Influx Assay*

Calcium influx was measured as previously described [19,20]. Briefly, cultured RBL-2H3 cells were removed and washed with PBS. After, cells were loaded with Fluo-4 AM (10 μM) (ThermoFisher) in buffer with Pluronic F-127 (0.01%) for 30 min at 37 ◦C. Subsequently, cells were washed twice with Tyrode's (TGB) buffer without calcium and equilibrated for 30 min at room temperature. Then, 5 × 10<sup>5</sup> cells/well were incubated in 96 wells black plates with PBS (negative control), LiRecTCTP (50 and 100 μg/mL), and LiRecTCTP (100 μg/mL) combined with cromolyn (10 μM) for 5, 15, 30, 60, and 90 min. The resulting fluorescence was quantified in Tecan Infinite M200 spectrofluorometer (Tecan, Männedorf, Switzerland) using an excitation wavelength of 485 nm and measuring emission at 535 nm. Calcium influx assay was performed in triplicate, and the results are shown as mean ± s.d. of three independent experiments.
