*2.15. Paw Edema-Forming Activity*

Paw edema development was measured at different time intervals as previously performed [2,26]. Briefly, groups of five mice (per condition) were treated with different types of inhibitors (as previously described in Pharmacological Treatments item) and were injected subcutaneously into the right hind paw with LiRecTCTP (10 μg dissolved in sterile PBS). Negative control mice were injected with the same volume of PBS (30 μL). Edema was evaluated by examining changes in paw thickness using a calibrated digital micrometer (Digimess, São Paulo, SP, Brazil) at the following time points: immediately after subcutaneous injection (t zero), 5, 10, 20, 30, 60, 120, 240, 360, and 720 min after injection. Results are shown as mean ± s.e.m of one representative experiment from three independent biological replicates.
