**2. Materials and Methods**

### *2.1. Yeast Strains, Media, and Growth Conditions*

Yeast strains used in this study are listed in Table 1. Yeast cells were grown in shaking flask at 30 ◦C in rich medium (YPD; 1% *w*/*v* yeas<sup>t</sup> extract, 2% *w*/*v* peptone, 2% *w*/*v* glucose) or defined synthetic medium (SD; 0.17% *w*/*v* yeas<sup>t</sup> nitrogen base, 0.5% *w*/*v* ammonium sulfate, 2% *w*/*v* glucose, and auxotrophic amino acids as required). Starvation experiments were performed in synthetic minimal medium lacking nitrogen (SD-N; 0.17% *w*/*v* yeas<sup>t</sup> nitrogen base without ammonium sulfate and amino acids, 2% *w*/*v* glucose).


**Table 1.** Yeast strains used in this study.
