*2.6. Cell Culture*

RBL-2H3 cell line was obtained as a courtesy of Professor Maria Celia Jamur (University of São Paulo, School of Medicine, Ribeirão Preto, SP, Brazil) and were grown in DMEM medium supplemented with 10% FCS (Gibco) and antibiotics penicillin (10,000 U/mL) (Sigma Aldrich) and streptomycin (10 mg/mL) (Sigma Aldrich). RBL-2H3 cells are a rat basophilic leukemia cell line used as a mast-like cell model [16]. RBL-2H3 cells grow as a monolayer, were subcultured (using trypsin/EDTA 2 mM, Gibco) and used until the 10th passage. Cell cultures were kept at 37 ◦C in a humidified atmosphere with 5% CO2.
