*2.13. Pharmacological Treatments (In Vivo)*

The following pretreatments were applied into mice in order to investigate LiRecTCTP effects on edema formation and vascular permeability in vivo: cromolyn (mast cell degranulation inhibitor), 30 mg/kg, administered i.p. in three consecutive days before exposure to LiRecTCTP; promethazine (histamine type 1 receptor (H1R) antagonist), 5 mg/kg, administered i.v. 30 min before exposure to LiRecTCTP; cimetidine (histamine type 2 receptor (H2R) antagonist), 15 mg/kg, administered i.p. 2 h before exposure to LiRecTCTP; thioperamide (histamine type 3 and 4 receptor (H3/H4R) antagonist), 20 mg/kg, administered i.p. 30 min before exposure to LiRecTCTP [23]. Sterile PBS was used as a negative control for pharmacological treatments and LiRecTCTP injections.
