*2.5. Circular Dichroism Spectroscopy (CD)*

Recombinant LiRecTCTP was dialyzed at 4 ◦C against a phosphate buffer (20 mM NaH2PO4/Na2HPO4 and 150 mM NaCl at pH 7.4) to a final concentration of 0.5 mg/mL. The UV-CD spectra were obtained in a Jasco J-810 spectropolarimeter (Jasco Corporation, Tokyo, Japan) using a 1 mm cuvette, as described previously [15]. The final spectrum (0.5 nm intervals) was the average of three measurements, performed at a rate of 50 nm/min, using a response time of 8 s and a bandwidth of 1 nm. The temperature was kept constant at 20 ◦C. The data units were expressed as molar ellipticity and plotted by GraphPad Prism 6 software. The results of deconvolution analyses and percentages of secondary structure were predicted by K2D3 tool (https://onlinelibrary.wiley.com/doi/abs/10.1002/prot.23188).
