*2.2. Plasmids*

The pAG32-*MMI1* plasmid was constructed by inserting a fragment containing 348 bps upstream of the *MMI1* ORF, *MMI1* ORF, and 454 bps downstream of *MMI1* ORF into the SacI and SpeI sites of the pAG32 plasmid. The fragment was amplified by PCR from pRS316-*MMI1* plasmid. Before the transformation of yeas<sup>t</sup> cells, the plasmid was cut with BsaAI restriction enzyme at the position of 319 bps downstream of *MMI1* ORF.

The pRS316-*MMI1* plasmid was made through PCR cloning. The DNA fragment containing 500 base pairs (bps) upstream of the start codon together with the *MMI1* ORF and 500 bps downstream of the ORF were amplified by PCR from WT yeas<sup>t</sup> genomic DNA. The fragment was then ligated into the SacI and EcoRI sites of pRS316 vector, resulting in the pRS316-*MMI1* plasmid.
