*2.1. Reagents*

The whole venom from *L. intermedia* was obtained by electrostimulation (15 V) of the cephalothorax of spiders, solubilized in PBS, and maintained frozen until use [13]. *L. intermedia* spiders were captured in the wild with the authorization of the Brazilian Governmental Agency "Instituto Chico Mendes de Conservação da Biodiversidade" (Number 29801-1). Ni2<sup>+</sup>-NTA agarose was purchased from Invitrogen (Carlsbad, CA, USA). DMEM media were purchased from Gibco (Carlsbad, CA, USA). The molecular mass markers were acquired from Sigma Aldrich (St. Louis, MO, USA). Evans Blue dye was purchased from Vetec (São Paulo, Brazil). The Compound 48/80, cromolyn sodium salt (cromolyn), promethazine hydrochloride (promethazine), cimetidine hydrochloride (cimetidine), and thioperamide maleate salt (thioperamide) were purchased from Sigma Aldrich. Ketamine and Sedanew ® (xylazin 10%) were from Agribands (Campinas, Brazil) and Univet (São Paulo, Brazil), respectively.
