*3.12. Proteolytic Digestion of Inclusion Bodies*

Purified PldA-GFP IBs were diluted to 1 OD at 350 nm in 980 mL of 50 mM Tris-HCl, 150 mM NaCl buffer of pH 8.0. Proteolytic digestion of IBs was initiated by adding 40 mL proteinase K (stock, 1 mg/mL) to the inclusion body solution (at 40 μg/mL final concentration). Proteolytic digestion was monitored for 120 min by measuring the changes in OD at 350 nm and in GFP fluorescence intensity (λexc. 488 nm/λexp. 530 nm).
