*4.8. Statistical Analysis*

All assays were performed in at least three independent experiments. Results are expressed as the mean ±standard deviation (SD). Student's t test was used to evaluate the data with the following significance levels: \* *p* < 0.05, \*\* *p* < 0.01, \*\*\* *p* < 0.001.

**Supplementary Materials:** The following are available online, Figure S1: The effect of rHct-S3 on proliferation of human colorectal carcinoma HT-29 cell lines.

**Author Contributions:** E.L., A.K., and O.M. designed the experiment and wrote the manuscript; A.K. and A.P. obtained recombinant analog of actinoporin; O.M., S.D. provided experiments on anticancer activity; E.L., S.E. and G.v.A. critically reviewed the manuscript. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the Russian Scientific Foundation, grant number 18-74-10028.

**Acknowledgments:** The authors are grateful to Pavel Dmitrenok for determination of molecular mass. The authors are grateful to Jessica Hauschild (University Medical Center Hamburg-Eppendorf) for the technical assistance of biological experiments.

**Conflicts of Interest:** The authors declare no conflict of interest.
