*3.4. Comparative Analysis of PL7-Containing Loci*

The genomic regions containing PL7 genes were extracted from the GBK files of the *Zobellia* genomes using Geneious Pro software (version 4.8) [45]. Due to the lack of genome annotations for *Zobellia* spp. and *Z. amurskyensis* MAR 2009 138, their genomes were initially annotated by the RAST server [46]. Identifiers for PL7 and adjacent genes included in selected loci are listed in Table S3. Generated GBK files were modified by adding custom color feature qualifiers. Pairwise comparisons of each locus between twelve genomes were carried out using BLASTn (BLAST version 2.11.0+) run in EasyFig (version 2.2.5) [47]. Synteny plots were visualized by Easyfig with the minimum BLAST hit of 680 bp.
