2.1.3. Sarcosyl Treatment

Extraction of the crude cell membranes of *M. primoryensis* KMM 3633T with 1% solution of Sarcosyl [24] resulted in an insoluble fraction of OM proteins. The precipitate obtained after washing with HEPES was a pure sample of the protein oligomer, whose electrophoretic mobility corresponded to MW about 95 kDa (Figure 1B, lane 1). The electrophoretic mobility of the monomer of the target protein under denaturing conditions corresponded to a polypeptide with a molecular weight of about 35 kDa (MpOmp) (Figure 1B, lane 2).
