*3.11. Solubility of PldA-GFP Ibs in Urea and SDS*

IBs were suspended in 50 mM Tris-HCl buffer, pH 8.0, containing SDS from 0.01 to 0.1% or urea from 1 to 8 M, and incubated for a specified time (from 5 min to 24 h). Turbidity of the samples was measured at 350 nm with a μQuant spectrophotometer (BioTEK Instruments, Inc., USA). Fluorescence intensity of the PldA-GFP IBs solutions in urea and SDS was recorded with a FL-600 Fluorescence/Absorbance Plate Reader (BioTEK Instruments, USA) with excitation wavelength at 495 nm and emission at 530 nm. The experiments were performed in three biological replicates. Results show the mean ± standard deviation (SD).
