*4.3. Cell Culture*

JB6 Cl41, HT-29, MDA-MB-231, and SK-MEL-28 cells were cultured in complete MEM/5% FBS, McCoy's 5A/10% FBS, DMEM/10% FBS, and MEM/10% FBS medium, respectively, containing 1% penicillin-streptomycin solution. The cell cultures were maintained at 37 ◦C in humidified atmosphere containing 5% CO2. Every 3–4 days, cells were rinsed with PBS, detached from the tissue culture flask by 0.25% trypsin/0.5 mM EDTA, and 10–20% of the harvested cells were transferred to a new flask containing fresh culture media. The cells after 4–5 passages were used for the experiments. The cells were passaged for a month.
