3.16.2. UV-Spectra

Absorption spectra were recorded using a UV-Visible spectrophotometer UV-1601 PC (Shimadzu, Japan) in quartz cuvettes with an optical path length of 1 cm, 0.1 cm, 0.01 cm in the range from 190 to 400 nm. Protein concentration of GFP in the PBS and PldA and PldA-GFP in the urea and SDS solutions was determined (following centrifugation at 15,000× *g* for 20 min) from the UV spectra at absorption maximum of 280 nm assuming that the A0.1% 1cm value was 2.74—for *Y.pseudotuberculosis* phospholipase A1; 1. 36—for PldA-GFP and 1.34—for GFP.
