2.1.1. Cultivation Condition of Microbial Cells

Bacterial cells of *M. primoryensis* KMM 3633<sup>T</sup> were grown at a temperature of 0, 6–8, and 24 ◦C using the growth medium described in [23]. A comparative analysis of the protein profiles of cell lysates obtained by ultrasonic disintegration was performed using SDS-PAGE (data not shown). Significant differences in the polypeptide composition of cell lysates from bacteria grown at a reduced temperature (0 and 6–8 ◦C) was not revealed. However, cultivation at room temperature led to some decrease in the intensity of polypeptide bands in the region of high molecular weight proteins. In accordance with the results obtained, for further studies, the microbial cells of M. primoryensis KMM 3633T grown at a temperature of 6–8 ◦C were used.
