4.3.2. Extraction with N-Lauroylsarcosine (Sarcosyl)

Cell envelope pellet (6.4 mg) was resuspended in 5 mL 10 mM HEPES, pH 7.5 (buffer B), containing 1% (*w*/*v*) Sarcosyl (Sigma-Aldrich, St. Louis, MO, USA) and incubated at room temperature for 30 min with shaking. The pellet obtained by centrifugation at 25,000× *g* was washed with 10 mL of buffer B without suspending. According to SDS-PAGE data, this fraction was electrophoretically pure 35 kDa protein (MpOmp) in oligomeric form (approximately 100 kDa).
