*2.2. Determination of Physicochemical Parameters*

Plasma samples were used in the determination of physicochemical parameters, including total proteins, aspartate transaminase (AST), albumin, alkaline phosphatase (ALP), alanine transaminase (ALT), and creatinine. The determination of main physicochemical parameters in this study, such as total proteins, albumin, AST, ALT, ALP, and creatinine, was performed in a dry automatic analyzer by microsides VITROS (Ortho Clinical Diagnostics, NJ, USA) operating by reflectance spectrophotometry.
