*2.1. Chemicals and Molecular Biology Supplies and Services*

Chemicals, unless otherwise indicated, were purchased from Sigma-Aldrich Chimie (Saint-Quentin-Fallavier, France). Unsulfated chondroitin was from HTL Biotechnology (Javené, France). DNA polymerases, DNA modification, and restriction enzymes were purchased from Thermo-Fisher Scientific Inc. (Illkirch, France). Synthetic DNA was purchased from GenScript Biotech (Leiden, The Netherlands). Chondroitin AC lyase from *Flavobacterium heparinum* and hyaluronidase from bovine testis were purchased from Sigma-Aldrich. DNA sequencing was performed by Eurofins Genomics (Ebersberg, Germany GmbH). Recombinant DNA was isolated using miniprep columns (Qiagen Inc., Valencia, CA, USA). The *E. coli* strain Top10 was used for plasmid construction and strain K-12 for recombinant polysaccharide production in bioreactor.
