*2.2. Sugar Feed*

Heparin directly dissolved at 5 mg/mL or 50 mg/mL in 10% glucose in H2O, or 10% glucose in H2O for the control group, were administered to mosquitoes twice (once each 24 h) in a cotton pad on the top of a net-capped paper cup containing 40–50 *A. stephensi* females. Mosquitoes were allowed to feed for 48 h, and then infected by direct feeding on a CD1 mouse parasitized with *Plasmodium berghei* ANKA-GFP (259cl1; MRA-865 [14]) for 10 min. Non-fed mosquitoes were removed, and fed mosquitoes were placed in the insectary at 21 ◦C and 75% humidity to allow parasite development. After eight days, mosquitoes were dissected, and the number of GFP-expressing oocysts per midgut was counted manually using an Axioskop fluorescence microscope (Zeiss, Oberkochen, Germany). The total number of mosquitoes analyzed was 61 in the control group, 63 treated with 5 mg/mL heparin, and 46 treated with 50 mg/mL heparin, distributed in three independent experiments.
