2.2.4. Cell Transfection

HUVEC were transfected with siRNA against syndecans4 (S12639, Ambion, Carlsbad, CA, USA) using a nucleofector apparatus (Amaxa, Basel, Switzerland) and the Amaxa HUVEC Nucleofector kit (Lonza, Basel, Switzerland) following the manufacturer's instructions. In total, 5 × 100,000 cells were resuspended in 100 µL HUVEC Nucleofector solution

and transfected with 40 nM siRNA against syndecans4 and Silencer Negative Control siRNA #1 (AM4611, Ambion, Carlsbad, CA, USA). Syndecans4 silencing efficiency was determined by qRT-PCR. Silenced cells were treated 24 h after the transfection.
