2.2.6. Migration Assay

HUVECs were cultured until confluence in 6-well plates and serum-deprived (0.2% FBS) for 16–18 h. Three scratches per well were done with a 20 µL sterile pipette tip. Cells were washed to remove debris and incubated in fresh M200 with or without TNF-α. Images from three different scratch areas in each culture well were obtained using Olympus (Hamburg, Germany) IX51 microscope after 2, 4, 6, and 8 h.
