*2.5. Degradation Method*

The degradation method of a single batch of the original sample HA-PLA DAC® from Novagenit is reported in the proprietary patent.

Degradation was obtained by 1.0 mL of NaOH 0.2 M solution heated at 60 ◦C, then 30 mg of sample is added stirring at constant temperature for 30 min. The aqueous phase is extracted from dichloromethane to eliminate free PLA produced by hydrolysis.
