*2.8. Statistical Analysis*

Statistical analysis was performed using GraphPad Prism version 7. The Shapiro–Wilk test was used to determine the normality of data. *T*-tests and Pearson's test were used to compare and correlate histology and immunohistochemistry scores, respectively. A two-way ANOVA with multiple comparisons was used to analyse the growth kinetics of the cells treated with heparanase and a paired, one-sample *t*-test for the fold change in gene expression. A *p*-value ≤ 0.05 was considered statistically significant.
