*2.10. Western Immunoblotting*

Samples were deposited onto polyacrylamide gels as previously described [35]. The GPC1 primary antibody used was 16700-1-AP (Proteintech, Rosemont, IL, USA). The appropriate peroxidase-coupled secondary antibody (1/10,000) was the anti-rabbit NA934V (GE Healthcare Life Sciences, Marlborough, MA, USA).
