*2.5. Enzymatic Degradation*

Chondroitin and chondbiuronan (500 µg) were treated by chondroitin AC lyase from *Flavobacterium heparinum* (0.9 mg) in 500 µL of 50 mM Tris-HCl buffer pH 8 at 25 ◦C. The reactions were followed by monitoring the formed unsaturated reducing ends at 235 nm.

Chondroitin and chondbiuronan (500 µg) were incubated at 37 ◦C with hyaluronidase from bovine testis (350 µg) in 250 µL of 0.1 M sodium acetate buffer pH 5 containing 0.1 M NaCl. At regular time intervals, the amount of reducing sugars was determined using the ferricyanide method [17].
