2.2.2. Hair Follicle Preparation for Infrared Analysis

For the different approaches described above, HFs were embedded individually in cryoprotective Tissue-Tek® O.C.T.™ Compound (Sakura, Alphen aan den Rijn, Netherlands) and snap-frozen at –80 ◦C. Seven micron-thick longitudinal sections of HFs were prepared using a cryostat (Leica Biosystems, Nanterre, France). The sections were then placed on an IR-transparent calcium fluoride (CaF2) window (Crystran Ltd., Dorset, UK) for IRSI analysis (2 to 4 sections per window). The sections were imaged directly without any staining.
