**3. Results and Discussion**

The present report describes the evolution of GAGs, sulfated GAGs, and, more specifically, the HS-type GAGs and GPC1 distribution in hair follicles at different phases of the hair growth cycle using spectral imaging. IRSI presents the advantage of being label-free, reagent-free, and rapid. The data obtained by this novel approach were compared to the immunohistochemistry and Western immunoblotting data.
