**5. Conclusions**

In this study, we investigated the supramolecular structuring of a HA/Ch complex using diverse analytical methods. The HA/Ch solution was characterized in terms of physicochemical properties. The supramolecular complex showed interesting rheological properties and promising preliminary evidence for biocompatibility—monodisperse molecular weight distribution, high viscosity, negative charge, degradation by specific enzyme, such as Hyaluronidase and Lysozyme. Due to the wide range of applications in biomedicine and biotechnology, the development of such polyelectrolyte complexes is of scientific and technological interest. Such an analytical approach would facilitate the development of new formulations, demonstrating the interactions with the different components.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/2218-273 X/11/3/389/s1, Figure S1: Schematic interaction between HA and Ch in Phosphate buffer saline.

**Author Contributions:** Conceptualization, S.B. and G.B.; investigation, R.L., I.T., C.A., and C.C.; writing—original draft preparation S.B. and G.B.; funding acquisition, G.B. All authors have read and agreed to the published version of the manuscript.

**Funding:** The research was partially funded by G. Ronzoni foundation.

**Data Availability Statement:** The data that support the findings of this study are available from the corresponding author upon reasonable request.

**Acknowledgments:** C.A. acknowledges partial support from the Italian Ministry for University and Research through the Rita Levi Montalcini fellowship for young researchers. C.A. and I.T. acknowledge technical support by Tiziano Catelani for SEM imaging. S.B., R.L., and C.C. acknowledge technical support by Regina Fratiello for HP-SEC-TDA analyses.

**Conflicts of Interest:** The authors declare no conflict of interest.
