*2.4. Detection of Heparin-Cy5 in Mosquitoes*

Thirty female *A. stephensi* mosquitoes per cage were allowed to feed on 400 µg/mL heparin-Cy5 (Nanocs Inc., New York, NY, USA) on either sugar for 6 h or MFA for 1 h. Some mosquitoes were taken from the cages at 6, 24, 48, and 72 h post-feeding to check Cy5 fluorescence (λex/λem: 650/670 nm) with an Eclipse 80i microscope (Nikon, Tokyo, Japan). Mosquitoes with fluorescent signal were dissected, and their organs were individually observed.
