*3.5. Extraction*/*Purification Procedures*

On day 10 of incubation, the resin/mycelium layer was recovered from the surface of the plates and re-suspended three times in 500 mL of Ethyl Acetate. Traces of water were removed on anhydrous sodium sulfate, and the solvent evaporated under reduced pressure. 550 mg of dry extract was recovered and submitted for analytical and structural analysis. The extract was fractionated by flash chromatography using a Combiflash-companion chromatogram, and the compounds were further purified by preparative HPLC to offer 6 to 56 mg of pure compound.
