*2.1. Genome Sequencing and Annotation of Streptomyces olivaceus SCSIO T05*

Whole genome sequence is important when analyzing the potential production of secondary metabolites [5,18]. *S. olivaceus* SCSIO T05, a marine-derived strain, was previously reported to be isolated from the Indian Ocean deep-sea-derived sediment [19]. Its draft genome sequence was first gained by Illumina sequencing technology, but with several gap regions. In order to estimate the biosynthetic potential of *S. olivaceus* SCSIO T05, the complete genome was re-sequenced and acquired by the single-molecule real-time (SMRT) sequencing technology (PacBio). A total of 67156 filtered reads with high-quality data of 432570025 bp were generated, and then they were assembled into a linear contig by the hierarchical genome assembly process (HGAP) [20]. The complete genome revealed that 8458055 base pairs constitute a linear chromosome without a plasmid, with 72.51% of GC content (Figure 1 and Table 1). Totally, 7700 protein-coding genes were predicted, along with 18 rRNA and 65 tRNA. The genome sequence of *S. olivaceus* SCSIO T05 was deposited in GenBank (CP043317).

**Figure 1.** The complete genome of *S. olivaceus* SCSIO T05. The three circles (inner to outer) represent forward GC content, GC skew, and the distribution of putative biosynthetic gene clusters (BGCs) (represented by the bars) generated by antiSMASH 5.0. Clusters 18, 17, and 11 were described as rishirilides, xiamycins, and mycemycins BGCs, respectively. The putative lobophorin BGC with red color was referred to as cluster 37.


**Table 1.** Genome features of *S. olivaceus* SCSIO T05.

AntiSMASH analysis by using antiSMASH 5.0 [21] suggested 37 BGCs within the *S. olivaceus* SCSIO T05 genome (Figure 1 and Table 2). The 37 BGCs totally occupy 1.59 Mb, 18.76% of the complete genome. Most of the BGCs distribute in the two subtelomeric regions of the genome of some *Streptomyces* strains [18] and so do the BGCs in *S. olivaceus* SCSIO T05 genome. It is predicted that several BGCs are responsible for the production of polyketide- and nonribosome-peptide-derived secondary metabolites, including four PKS (Type I, Type II and Type III) and six NRPS, and six hybrid BGCs possess genes encoding more than one type of scaffold-synthesizing enzyme. Twenty-one BGCs are predicted to produce terpene, bacteriocin, lanthipeptide, or other categories. This analysis indicates that *S. olivaceus* SCSIO T05 is capable of producing an array of secondary metabolites, serving as a target strain for further metabolic engineering and genome mining.


**Table 2.** AntiSMASH-predicted BGCs for *S. olivaceus* SCSIO T05.


**Table 2.** *Cont.*
