**Gene Expression of Transient Receptor Potential Channels in Peripheral Blood Mononuclear Cells of Inflammatory Bowel Disease Patients**

**Taku Morita 1, Keiichi Mitsuyama 1,2,\*, Hiroshi Yamasaki 1,2, Atsushi Mori 1,2, Tetsuhiro Yoshimura 1,2, Toshihiro Araki 1,2, Masaru Morita 1,2, Kozo Tsuruta 1,2, Sayo Yamasaki 1, Kotaro Kuwaki 1,2, Shinichiro Yoshioka 1,2, Hidetoshi Takedatsu 1,2 and Takuji Torimura 1**


Received: 11 July 2020; Accepted: 12 August 2020; Published: 14 August 2020

**Abstract:** We examined the expression profile of transient receptor potential (TRP) channels in peripheral blood mononuclear cells (PBMCs) from patients with inflammatory bowel disease (IBD). PBMCs were obtained from 41 ulcerative colitis (UC) patients, 34 Crohn's disease (CD) patients, and 30 normal subjects. mRNA levels of TRP channels were measured using the quantitative real-time polymerase chain reaction, and correlation tests with disease ranking, as well as laboratory parameters, were performed. Compared with controls, TRPV2 and TRPC1 mRNA expression was lower, while that of TRPM2, was higher in PBMCs of UC and CD patients. Moreover, TRPV3 mRNA expression was lower, while that of TRPV4 was higher in CD patients. TRPC6 mRNA expression was higher in patients with CD than in patients with UC. There was also a tendency for the expression of TRPV2 mRNA to be negatively correlated with disease activity in patients with UC and CD, while that of TRPM4 mRNA was negatively correlated with disease activity only in patients with UC. PBMCs from patients with IBD exhibited varying mRNA expression levels of TRP channel members, which may play an important role in the progression of IBD.

**Keywords:** crohn's disease; mononuclear cells; transient receptor potential channel; ulcerative colitis
