*2.4. DNA Pyrosequencing*

Equal concentrations of the PCR products were pooled together and cleaned with Agencourt Amperes magnetic purification beads (Danvers, MA, USA). The quality and size of the pooled products were assessed on an Agilent Bioanalyzer 2100 (Palo Alto, CA, USA) using a DNA 7500 chip. The pooled products were used for emulsion PCR and then loaded onto Picotiter plates for high-throughput pyrosequencing. The sequencing was performed on the GS FLX Plus system (Roche, Branford, CT, USA) according to the manufacturer's instructions at ChunLab, Inc. (Seoul, Korea).
