4.4.3. Parkinsonian Syndrome Rat Model

The rats were anesthetized intraperitoneally with 0.06 mL/10 g of Zoletil 50 (50 mg/mL) dilute 5X (1 mL Zoletil in 0.1 mL Rompun 2% and 3.9 mL normal saline). The rats were then fixed in a stereotactic position. The rats' hair was shaved off, they were disinfected with povidone-iodine, and the skull was exposed. We injected into the right substantia nigra 3 μL LPS (5 μg/μL) (from *Escherichia coli* 0111:B4, Sigma, St. Louis, MO, USA). To achieve the nigrostriatal system s unilateral lesion, a microsyringe was used with a flow rate of 1 μL/min. The needle was held in the head for 7 min to prevent reflux of the drug. The skin was sutured for observation.
