*4.12. Statistical Analysis*

All data are presented as mean ± standard error of the mean (SEM). Data analysis were performed with a one-way analysis of variance (ANOVA) followed by a post hoc Bonferroni test. Analysis and graphical presentation were conducted with the GraphPad Prim Software version 8 (GraphPad Software Inc., San Diego, CA, USA). Statistical significance was achieved when *p* < 0.05.

**Author Contributions:** Conceptualization, P.A.D., J.D.A., C.A.M.A. and C.M.P.R.; methodology, S.R.O., P.A.D., M.M.G., M.B.T.F., C.A.B.R., R.G.P., M.S.E. and M.J.P.; investigation, S.R.O., P.A.D., M.M.G., M.B.T.F., C.A.B.R., R.G.P., M.S.E. and M.J.P.; data curation, S.R.O., P.A.D., J.D.A., R.M. and C.A.M.A.; writing—original draft preparation, S.R.O.; writing—review and editing, S.R.O., P.A.D., M.M.G., M.B.T.F., C.A.B.R., R.G.P., M.S.E., M.J.P., R.M., C.A.M.A., J.D.A. and C.M.P.R.; visualization, S.R.O., J.D.A. and C.A.M.A.; supervision, J.D.A., C.A.M.A. and C.M.P.R.; project administration, J.D.A. and C.M.P.R.; funding acquisition, C.M.P.R. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received funding from European Structural & Investment Funds through the COMPETE Program—Programa Operacional Regional de Lisboa—Program Grant LISBOA-01-0145- FEDER-016405, and from National Funds through the FCT—Fundação para a Ciência e a Tecnologia— Program Grant SAICTPAC/0019/2015. S.R.O. received a PhD fellowship (PD/BD/128332//2017) from the FCT.

**Institutional Review Board Statement:** The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the institutional Review Board and by the national authority Direção-Geral de Alimentação e Veterinária (DGAV), initiated on 1 August 2018 (Necroptosis in dopaminergic neurodegeneration in Parkinson's disease), in accordance with the EU Directive (2010/63/UE), Portuguese laws (DL 113/2013, 2880/2015, 260/2016 and 1/2019) and all relevant legislation.

**Informed Consent Statement:** Not applicable.

**Acknowledgments:** We thank all members of the laboratory for advice and technical assistance.

**Conflicts of Interest:** The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
