*2.2. In Vitro Cell Analysis*

#### 2.2.1. Cell Viability

The cell viability of cells treated with EGCG from 50 to 400 μM was significantly decreased compared to the control group (Figure 1A). In contrast, the viability of cells receiving 5 to 25 μM is similar to the control group cells' viability. The concentration of EGCG used in this study was determined to be 25 μM. Similarly, the concentration of LPS used for induction of cell inflammation was determined to be 50 ng/mL according to Figure 1B. In Figure 1C, the cell viability of cells co-cultured with placebo liposomes of all concentrations was not statistically significant compared to the control group. Therefore, placebo liposomes are not cytotoxic to microglia cells.

**Figure 1.** Cell viability of BV-2 cells treated by (**A**) EGCG, (**B**) lipopolysaccharide (LPS), and (**C**) placebo liposome (compared with control group (CTL), \* *p* < 0.05; \*\*\* *p* < 0.001, \*\*\*\* *p* < 0.0001, *n* = 3).
