TNF-*α* Assessment

We cultured BV-2 cells in 6-well plates. Cells were then treated with 125 μg/mL EGCGloaded liposomes or placebo and incubated for one hour. We induced the inflammation by treating the cells with 50 ng/mL LPS and incubating them for 24 h. The culture media were collected for TNF-α secreted assessment using TNF-α mouse ELISA assay kit (R&D Systems' biotechnology brand).
