4.3.3. Cell Morphology

BV-2 cells were cultured in a 6-well plate (105 cells/ cm2) for 24 h to stabilize cell attachments. We established various culture conditions to monitor cell morphology: (i) cells treated with 25 μM EGCG; (ii) cells treated with 50 ng/mL LPS; and (iii) cells pretreated with EGCG for 1 h, followed by 50 ng/mL LPS treatment. The inverted microscope (Nikon Eclipse 50i, Tokyo, Japan) was used.
