*4.2. Polymerase Chain Reaction*

In the transgenic animals, the presence of TDP-43G348C transgene was determined by polymerase chain reaction (PCR) as previously described [28]. GoTaq® G2 Green Master Mix (Promega Corporation, Madison, WI, USA) and the primers CTCTTTGTGGAGAG-GAC and TTATTACCCGATGGGCA (Metabion international AG, Planegg, Germany) were used for the reaction.
