4.4.5. Animal Inflammatory Factor Analysis

Rats after the rotational test were anesthetized, sacrificed, and perfused. The brain's damaged nigrostriatal region was collected for RNA extraction with 1 mL TRIzol® (Invitrogen, Carlsbad, CA, USA) reagent according to the manufacturer's instruction. The RNA concentration was measured using a micro-volume spectrometer. Real-time reverse transcription-polymerase chain reaction (qRT-PCR) was performed by SuperScript III First-Strand Synthesis SuperMix and analyzed by 7300 Real-Time PCR System (Applied Biosystems, USA). cDNA was generated by reverse transcription of 1 μg RNA with reverse transcriptase (RT) and oligo(dT) primers (Table 3). The cDNA was diluted to 2.5 ng/μL, and cDNA, primers (Table 3), and FastStart Universal SYBR® Green Master (Rox) (Roche, Germany) were added to a qPCR 8-strip tube for the qPCR reaction.


