*2.6. Statistical Correlation between ROS, NO, DBSs and S100B Results*

Correlations between extracellular and cellular S100B protein levels and DNA damage, ROS or NO levels, analyzed by using Pearson correlation coefficients, are shown in Table 2.

**Table 2.** Correlations between 100B protein levels and measured parameters: ROS, NO and DBSs in neuron-like cells after glucose or insulin treatment for 24 h.


Based on the calculated Pearson correlation coefficients in almost all glucose concentration tested after 24 h incubation, correlations between the results of intracellular and extracellular levels of S100B protein were negative compared to outcomes from ROS, NO and DBSs levels. The obtained correlation was strong only in the case of extracellular S100B levels vs. ROS levels, whereas after insulin administration, a strong correlation was observed only between intracellular S100B protein levels and DBSs or NO as well as among extracellular S100B protein levels and DBSs or NO. To reiterate, the correlation between extracellular and intracellular S100B levels and DNA damage or NO levels after insulin administration was strong. Such a consequent effect was not observed in a hyperglycemic environment.
