*4.5. Strand-Specific RT-PCR*

To evaluate the replication of the detected viruses, strand-specific RT-PCRs were performed using specific primers, as previously described [47]. All cDNAs were amplified by PCR for the related viral target. The amplicons were detected on a 2% agarose gel, sequenced (BMR Genomics, Padua, Italy), and analyzed using BLAST [87]. Phylogenetic analysis was performed using the maximum likelihood method based on the Tamura–Nei model using MEGA software [88].
