*3.6. Reactive Oxygen Species Assay*

2- ,7- -Dichlorodihydrofluorescein diacetate (H2DCFDA) was used to measure ROS generated in the reactions between oxyresveratrol in the presence or absence of copper (II) ions. The reaction was initiated by adding 50 μL of 200 μM Cu(II) or HEPES buffer into each reaction of 200 μL that contained oxyresveratrol or other derivatives. After the addition, the tubes were incubated at 37 ◦C for 15 min. Dichlorofluorescein (DCF), the fluorescence product of the reaction, was quantitated spectrofluorometric.
