*4.3. RNA-Scope*

To visualize the subcellular localization of *LINC01133* RNA in tissue samples of women with endometriosis we used the RNAscope® 2.5 HD Red assay on formalin-fixed paraffinembedded eutopic endometrial tissues of women with endometriosis (*n* = 5), according to the manufacturer's protocol (Advanced Cell Diagnostics (ACD), Hayward, CA, USA). This method uses a signal amplification method and double Z probe design provides high sensitivity and specificity suitable for detecting relatively lowly expressed RNAs, such as lncRNAs. The system visualizes target RNA as a single dot, where each dot is an amplified signal of an individual RNA molecule. We used the probe Hs-LINC01133 designed by ACD to specifically detect *LINC01133*, and probe dapB (bacterial dihydrodipicolinate reductase, PN310043) as a negative control.
