*4.4. mRNA and miRNA Expression in Tissues and PF-Treated Endometrial Cells*

RNA (which includes miRNA) isolated from the tissues and treated cells were used. cDNA synthesis from 1 µg of each sample was performed using iScript cDNA synthesis kit (Cat No: 1708890, Biorad, Hercules, CA, USA). mRNA expression was analyzed in the cDNA samples using SYBR Green (Cat No: 1725270, Biorad, Hercules, CA, USA) and the primers listed in Table S1. 18S was used for normalization of mRNA expression. For determining miRNA expression, cDNA synthesis from 2 µg of each sample was performed using miScript II RT Kit (Cat No: 218161, Qiagen, Gaithersburg, MD, USA). Following cDNA synthesis, the expression of miR-29a, miR-148a, and miR-155 in tissues and PF-treated cells were determined using the appropriate Qiagen Primer Assay Kit, as per the manufacturer's instructions. A primer assay for RNU6 was used as housekeeping for miRNA expression.
