*4.4. LINC01133 Knockdown*

The 12Z cells were seeded in complete culture cell medium 12 h prior to transfection on 6-well culture plates (Nunc, Thermo Fisher Scientific, Waltham, MA, USA) at a concentration of 1 <sup>×</sup> <sup>10</sup><sup>5</sup> cells/well and allowed to grow to approximately 40% confluency. The cells were then transfected with one of three different LINC0133 targeting siRNA oligos at a final concentration of 10 nM (Table S2A) or a non-targeting control siRNA oligo (Cat.4390846, Ambion, Austin, Texas, USA) using Lipofectamine RNAiMAX transfection reagent according to the manufacturer's protocol (Invitrogen by Life Technology, Waltham, MA, USA). Phenotypic analysis of *LINC01133* knockdown cells for changes in cellular proliferation and invasion/migration was conducted 48 h post-transfection. RNA-sequencing, qRT-PCR and Western blot analysis were conducted 72 h post-transfection. The 3 siRNAs had similar *LINC01133* knockdown efficiencies (Figure 2A). Therefore, for each experiment, we first checked *LINC01133* knockdown efficiency by qRT-PCR, and then proceeded with the 2 siRNAs that showed the greatest knockdown efficiency.
