*4.7. EpiTect Methylation Array*

An EpiTect Methyl II Complete PCR Array (Cat No: 335005, Qiagen, Gaithersburg, MD, USA) was used to examine the levels of methylation in genes involved in inflammation and autoimmunity, in PF treated Ishikawa endometrial cells. Cells were treated as previously described and collected. DNA was isolated from the cells and DNA quantity and quality of RNA were measured using the NanoDrop 2000 spectrophotometer (Cat No: ND2000, Thermo Scientific, Waltham, MA, USA). Protocol provided by the manufacturer was followed. Input DNA was obtained and aliquoted into four equal portions and subjected to mock, methylation sensitive, methylation-dependent, and double restriction endonuclease digestion. After digestion, the enzyme reactions were used for qPCR using Sybrgreen. Analysis was performed using algorithm provided by Qiagen/SA Biosciences (Gaithersburg, MA, USA). A heat map was created from the data provided using Prism software (Version 9.0.0) (GraphPad, Inc., La Jolla, CA, USA).
