*4.4. Cytokine Evaluations*

Concentrations of cytokines (IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, and TNF) and chemokines (CCL2, CCL5, CXCL8, and CXCL9) in peritoneal fluids and culture media were measured using the BD™ Cytometric Bead Array (CBA) Human Th1/Th2/Th17 Cytokine and Human Chemokine kits (BD Bioscience, USA), respectively. The samples were evaluated using a FACSVerse flow cytometry with BD Suite software (BD Bioscience) according to the protocol provided by the manufacturer. The results were analyzed with FCAP Array software (BD Bioscience). The advertised theoretical limit of detection defined as the corresponding concentration at two standard deviations above the median fluorescence of 20–30 replicates of the negative control (0 pg/mL) for IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, and TNF was 2.6, 4.9, 2.4, 4.5, 18.9, 3.7, and 3.8 pg/mL, respectively. The respective theoretical limit of detection for CCL2, CCL5, CXCL8, and CXCL9 was 2.7, 1.0, 0.2, and 2.5 pg/mL. The measurements were always within the respective standard curve. Raw data of standard curves for all assays are shown in a supplementary file.
