**4. Materials and Methods**

#### *4.1. Study Sites*

The fecal samples of pet dogs and cats were collected in Kunming city, Lijiang city and Chuxiong city in Yunnan province (Location: 21◦8 N to 29◦15 N and 97◦31 E to 106◦11 E), southwestern China, which covers more than 390,000 square kilometers and has a population of approximately 48 million.
