*4.6. Nucleotide Sequence Accession Numbers*

Here, the representative nucleotide sequences of this study were deposited in the Gen-Bank database under the following accession numbers: MW322754–MW322755, MW321613– MW321626.

#### **5. Conclusions**

This work presents the first report of *G. duodenalis* infections in coypus in China based on MLG analysis. Multi-locus genotyping yielded 10 novel MLGs, including one assemblage A MLG (AI–novel 1) and nine assemblage B MLGs (BIV–novel 1 to 7). Of note, the presence of zoonotic assemblages and sub-assemblages of *G. duodenalis* in coypus suggests the potential contribution of these animals to human giardiasis transmission.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/2076-081 7/10/2/179/s1, Table S1 Primer sequences and reaction conditions used in nested PCR amplifications. Figure S1. Genomic map of the positions of *tpi*, *gdh* and *bg* genes.

**Author Contributions:** Conceptualization, M.Q. and L.Z.; methodology, M.Q. and L.Z.; validation, M.Q. and L.Z.; formal analysis, Z.C. and D.W.; investigation, W.W., Y.Z., B.J. and C.X.; software, C.X. and Y.C.; resources, M.Q.; data curation, Z.C.; writing—original draft preparation, Z.C.; writing review and editing, M.Q. and Z.C.; visualization, M.Q. and L.Z.; supervision, M.Q. and L.Z.; project

administration, M.Q. and L.Z.; funding acquisition, M.Q. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the Program for Young and Middle-Aged Leading Science, Technology, and Innovation of Xinjiang Production & Construction Corps (2018CB034).

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Acknowledgments:** We are grateful to Robiul Karim from Bangabandhu Sheikh Mujibur Rahman Agricultural University for his cordial help in editing the English text of a draft of this manuscript.

**Conflicts of Interest:** The authors declare no conflict of interest.

#### **References**

