*3.2. Cryptosporidium Species Identified*

Species identification was successful for 95% (379/398) of the samples, and 12 different species/genotypes were identified (Table 2). In total, 370 of the isolates were identified by RFLP and/or sequencing of the SSU rRNA gene, and 4 by sequencing of the *hsp70* gene (3 *C. parvum* and 1 *C. meleagridis*). For five samples with negative SSU rRNA PCR, species determination was based on the positive *gp60* result (4 *C. parvum* and 1 *C. hominis*). Of 19 samples that were PCR-negative and therefore not typeable (Tables 1 and 2), 14 had been preserved in SAF. *Cryptosporidium parvum* was the species most commonly observed (79%; 299/379), followed in frequency by *C. hominis* (13%; 49/379), *C. meleagridis* (*n* = 8), *C. cuniculus* (*n* = 5), *Cryptosporidium* chipmunk genotype I (*n* = 5), *C. felis* (*n* = 4), *Cryptosporidium erinacei* (*n* = 2), *C. ubiquitum* (*n* = 2), and 1 each of *Cryptosporidium suis*, *C. viatorum*, *Cryptosporidium ditrichi*, and *Cryptosporidium* horse genotype. One patient was co-infected with *C. parvum* and *C. hominis*, as evidenced by RFLP analysis of the SSU RNA gene followed by Sanger sequencing of the same gene.

**Table 2.** Distribution of *Cryptosporidium* spp. according to area of origin of infection among 398 patients with cryptosporidiosis diagnosed in Sweden from 2013 to 2014.


<sup>1</sup> Negative in all PCRs.
