*Article* **The Effect of Different Cleaning Methods on Protein Deposition and Optical Characteristics of Orthokeratology Lenses**

**Chen-Ying Su 1,†, Lung-Kun Yeh 2,3,†, Yi-Fei Tsao <sup>1</sup> , Wen-Pin Lin 4,5, Chiun-Ho Hou 2,3, Hsueh-Fang Huang <sup>4</sup> , Chi-Chun Lai 2,3 and Hsu-Wei Fang 1,6,\***


**Abstract:** Orthokeratology lenses are commonly used for myopia control, especially in children. Tear lipids and proteins are immediately adsorbed when the lens is put on the cornea, and protein deposition may cause discomfort or infection. Therefore, we established an in vitro protein deposition analysis by mimicking the current cleaning methods for orthokeratology lens wearers for both shortterm and long-term period. The results showed that the amounts of tear proteins accumulated daily and achieved a balance after 14 days when the lens was rubbed to clean or not. Protein deposition also affected the optical characteristics of the lens regardless of cleaning methods. Our results provided an in vitro analysis for protein deposition on the lens, and they may provide a potential effective method for developing care solutions or methods that can more effectively remove tear components from orthokeratology lenses.

**Keywords:** orthokeratology lens; protein deposition; optical characteristics; rubbing
